Alterations of maternal plasma HTRA1 level in preeclampsia complicated by IUGR

Marzioni, Daniela; Lorenzi, Teresa; Altobelli, Emma; Giannubilo, Stefano Raffaele; Paolinelli, Francesca; Tersigni, Chiara; Crescimanno, Caterina; Monsurró, Vladia; Tranquilli, Andrea Luigi; Simone, Nicoletta Di; Castellucci, Mario

doi:10.1016/j.placenta.2012.09.011

Cited by 24 publications

(16 citation statements)

References 17 publications

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“…While previous studies reported the detection of HtrA1 in pregnant sera [31,32,35], the reported levels were very low (<10 ng/ml) with a commercial kit, which in our test did not work with serum samples. Our newly established and thoroughly validated ELISA detected average levels of 30, 80 and 200 ng/ml of HtrA1 in sera from 1st, 2nd and 3rd trimesters respectively.…”

Section: Discussioncontrasting

confidence: 61%

“…HtrA1 protein has also been reported to be elevated in sera of PE patients [31,32]. One of these studies used a commercial enzymelinked immunosorbent assay (ELISA) kit to determine serum HtrA1 [31].…”

Section: Introductionmentioning

confidence: 99%

“…One of these studies used a commercial enzymelinked immunosorbent assay (ELISA) kit to determine serum HtrA1 [31]. However, the levels reported were very low, and the authors noted that the study was preliminary.…”

Section: Introductionmentioning

confidence: 99%

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Serum HtrA1 is differentially regulated between early-onset and late-onset preeclampsia

et al. 2015

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This is the first report to show a clear increase of HtrA1 in the maternal circulation during normal pregnancy, consistent with HtrA1 being highly expressed in the placenta. Importantly, this study identified that serum HtrA1 was altered differently in early-onset and late-onset PE pregnancies, highlighting the complex regulation of HtrA1 in the different subtypes. The significant increase of serum HtrA1 in early-onset PE suggests that it may be a potential biomarker for the diagnosis of early-onset PE at disease presentation.

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Section: Discussioncontrasting

confidence: 61%

Section: Introductionmentioning

confidence: 99%

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Serum HtrA1 is differentially regulated between early-onset and late-onset preeclampsia

et al. 2015

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“…CD may be abundantly expressed in placenta, such that circulating levels are lower, as was the case in our study. A previous study on high-temperature requirement A1 (HtrA1), a serine protease secreted from the placenta, showed that its concentration in maternal plasma is inversely correlated with its placental expression [26].…”

Section: Discussionmentioning

confidence: 99%

Elevated serum cathepsin B concentration in pregnant women is associated with preeclampsia

Kim

2016

Arch Gynecol Obstet

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“…18 Preeclamptic placentas display alterations in the global redox balance, and the levels of antioxidant molecules (TRX and glutaredoxin) are elevated in response to increased oxidative insults, 43,44 thereby providing a possible explanation for the enhanced HtrA1 autolysis. In general, plasma levels of all HtrA family members are regulated during gestation, 45 and an autolytic form of HtrA3 (contains an N-domain similar to HtrA1) can be observed in plasma, suggesting that autolysis goes beyond the intracellular environment. 46,47 For HtrA1, it still remains to be established whether direct correlations exist between autolysis and intra- or extracellular location and how functional differences of the forms play a role.…”

Section: Discussionmentioning

confidence: 99%

The Autolysis of Human HtrA1 Is Governed by the Redox State of Its N-Terminal Domain

et al. 2014

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Human HtrA1 (high-temperature requirement protein A1) belongs to a conserved family of serine proteases involved in protein quality control and cell fate. The homotrimeric ubiquitously expressed protease has chymotrypsin-like specificity and primarily targets hydrophobic stretches in selected or misfolded substrate proteins. In addition, the enzyme is capable of exerting autolytic activity by removing the N-terminal insulin-like growth factor binding protein (IGFBP)/Kazal-like tandem motif without affecting the protease activity. In this study, we have addressed the mechanism governing the autolytic activity and find that it depends on the integrity of the disulfide bonds in the N-terminal IGFBP/Kazal-like domain. The specificity of the autolytic cleavage reveals a strong preference for cysteine in the P1 position of HtrA1, explaining the lack of autolysis prior to disulfide reduction. Significantly, the disulfides were reduced by thioredoxin, suggesting that autolysis of HtrA1 in vivo is linked to the endogenous redox balance and that the N-terminal domain acts as a redox-sensing switch.

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Alterations of maternal plasma HTRA1 level in preeclampsia complicated by IUGR

Cited by 24 publications

References 17 publications

Serum HtrA1 is differentially regulated between early-onset and late-onset preeclampsia

Serum HtrA1 is differentially regulated between early-onset and late-onset preeclampsia

Elevated serum cathepsin B concentration in pregnant women is associated with preeclampsia

The Autolysis of Human HtrA1 Is Governed by the Redox State of Its N-Terminal Domain

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