Alterations of organ histopathology and metallolhionein mRNA expression in silver barb, Puntius gonionotus during subchronic cadmium exposure

Wangsongsak, Alisa; Utarnpongsa, Suwimol; Kruatrachue, Maleeya; Ponglikitmongkol, Mathurose; Pokethitiyook, Prayad; Sumranwanich, Thitinun

doi:10.1016/s1001-0742(07)60219-8

Cited by 37 publications

(20 citation statements)

References 27 publications

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“…Gill damage has been reported in Puntius gonionotus, Sparus aurata and by [36,37] . In the same line, sodium and chloride levels as well as plasma osmolality were significantly reduced in C. carpio exposed to cadmium [28] .…”

Section: Discussionmentioning

confidence: 99%

Immunological, hematological and biochemical changes induced by short term exposure to cadmium in catfish (Clarias gariepinus)

2014

JCLM

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Section: Discussionmentioning

confidence: 99%

Immunological, hematological and biochemical changes induced by short term exposure to cadmium in catfish (Clarias gariepinus)

2014

JCLM

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“…Earlier laboratory studies by Kovarova et al (2009), in which the hepatic MT protein in carp reached its maximum level (>130 ng/g) in lower exposure to Cd (2.5, 5, 7.5 mg/L CdCl) yet hit the low MT levels (<50 ng/g) in conditions with the highest exposure to Cd (12.5 mg/L CdCl) and control groups (Cd-free treatment) in all time periods of exposure (24,48,72, and 96 h). Wangsongsak et al (2007) also demonstrated that during 56 days of exposure of silver barb to three series amounts of Cd, i.e., 0.012, 0.06, and 0.12 mg/L, consecutively, the hepatic and renal MT mRNA expression increased significantly upon the exposure and reached its highest expression at 28 days. However, MT expressions both in liver and kidney gradually dropped after 35 days.…”

Section: Discussionmentioning

confidence: 75%

“…Metallothionein is expected to be induced under conditions of elevated metal concentrations, allowing more binding sites to sequester the metal ions and preventing potential adverse effects (Langston et al, 2002). Some studies in a variety of fish such as killifish (Van Cleef-Toedt et al, 2001), silver barb (Wangsongsak et al, 2007) and grass carp (Tan et al, 2016) showed the elevation of MT expression level after being exposed to Cd. In the current study, the highest MT gene expression of the liver was found in site S3 of 5.92-fold followed by site S2 of 1.25-fold compared to site S1 (reference site).…”

Section: Discussionmentioning

confidence: 99%

“…The non-MT factors induced by metal might act on their own or in concert with MT to inhibit the activation potential (Koropatnick and Zalups, 1997). MT was found to be strongly induced by Cd in the laboratory (Wangsongsak et al, 2007) and field condition (Fernandes et al, 2008); however, not every species of fish is suitable for bio-monitoring (Kovarova et al, 2009). It was reported that MT was suitable as a bioindicator for monitoring heavy metal pollution in brown trout, but not in European eel (Linde et al, 2001).…”

Section: +mentioning

confidence: 99%

“…When the liver reaches saturation of Cd, Cd-MT complexes are then released into the blood stream and are delivered to the kidneys as the main storage site of Cd (Klaassen et al, 2009). MTs are strongly induced by heavy metals such as Cd; therefore, they are considered as a biomarker of metal exposure (Wangsongsak et al, 2007;Ivanina et al, 2008). The remarkable characteristic of all MTs in the protein level is the presence of Cys-Cys, Cys-X-Cys, and Cys-X-X-Cys structural motifs, where X is the amino acid other than Cys .…”

Section: Introductionmentioning

confidence: 99%

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Research Article Cadmium accumulation and metallothionein gene expression in the liver of swamp eel (Monopterus albus) collected from the Mae Sot District, Tak Province, Thailand.

Wahid¹,

Prasarnpun²,

Yimtragool³

2017

Genet. Mol. Res.

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ABSTRACT. Cadmium (Cd) is produced mainly as a by-product of zinc mining. In Thailand, the largest zinc mine is located in the Mae Sot district, Tak Province. Samples of Monopterus albus were collected from paddy fields in 4 sites, three downstream and one upstream from the zinc mine. The upstream site was considered to be uncontaminated while the three downstream sites were considered to be contaminated with Cd. Studies on the accumulation level of cadmium were conducted on the liver of the fish using the atomic absorption spectrophotometer technique. The metallothionein (MT) gene expression level in the liver, as a potential biomarker for long-term Cd exposure in their natural habitat, was also assessed. The level of hepatic MT gene expression was performed by quantitative real-time PCR. The result showed that Cd accumulation in the liver was much higher in swamp eels collected from the downstream sites when compared to those collected from the upstream site. The hepatic MT level in the upstream site was 0.75-fold, 2 M. Wahid et al. Genetics and Molecular Research 16 (3): gmr16039748 while the other three downstream sites were 0.36-, 4.44-and 0.94-fold. There is no parallel correlation between hepatic cadmium levels and hepatic MT gene expression. This study then suggests that MT gene expression biomarkers might be not suitable for swamp eels with prolonged exposure to Cd.

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Ultimobranchial gland of a freshwater teleost, Heteropneustes fossilis, in response to cadmium treatment

Rai

Mishra

Srivastav

et al. 2008

Environmental Toxicology

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Heteropneustes fossilis were subjected to 288 mg/L (0.8 of 96 h LC(50)) and 72 mg/L (0.2 of 96 h LC(50)) of cadmium chloride for short-term and long-term experiments, respectively. After sacrificing the fish, the blood was collected on 24, 48, 72, and 96 h in short-term and after 7, 14, 21, and 28 days in long-term experiment and analyzed for plasma calcium levels. Also, ultimobranchial glands were fixed on these intervals. The plasma calcium levels of short-term cadmium-exposed fish remain unchanged after 24 h. The levels exhibit a progressive decrease from 48 h onwards. The fish exposed to cadmium for 7 days exhibit a decrease in the plasma calcium level. Thereafter, the levels progressively decrease till the end of the experiment (28 days). Up to 72 h exposure of the fish to cadmium, the ultimobranchial gland exhibits no histological change. After 96 h, a decrease in the staining response of the cytoplasm of ultimobranchial cells has been noticed. The nuclear volume of these cells records a slight decrease. Up to 14 days of cadmium exposure, there is no change in the histological structure of ultimobranchial gland. After 21 days following the exposure, the ultimobranchial cells exhibit a slight decrease in the staining response of the cytoplasm and the nuclear volume of these cells records a decrease. Following 28 days cadmium exposure the nuclear volume exhibits a further decrease, and degeneration and vacuolization sets in.

show abstract

Alterations of organ histopathology and metallolhionein mRNA expression in silver barb, Puntius gonionotus during subchronic cadmium exposure

Cited by 37 publications

References 27 publications

Immunological, hematological and biochemical changes induced by short term exposure to cadmium in catfish (Clarias gariepinus)

Immunological, hematological and biochemical changes induced by short term exposure to cadmium in catfish (Clarias gariepinus)

Research Article Cadmium accumulation and metallothionein gene expression in the liver of swamp eel (Monopterus albus) collected from the Mae Sot District, Tak Province, Thailand.

Ultimobranchial gland of a freshwater teleost, Heteropneustes fossilis, in response to cadmium treatment

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