Alterations of Primary Metabolites in Root Exudates of Intercropped Cajanus cajan–Zea mays Modulate the Adaptation and Proteome of Ensifer (Sinorhizobium) fredii NGR234

Vora, Siddhi M.; Ankati, Sravani; Patole, Chhaya; Podile, Appa Rao; Archana, G.

doi:10.1007/s00248-021-01818-4

Cited by 13 publications

(8 citation statements)

References 88 publications

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“…Guo [10] and other mechanisms play a vital role in the regulation of plant growth, health, yield and other aspects [1] [11]. Root exudates are important intermediates in the interaction between rhizosphere microorganisms and plants [12] [13]. To study the interaction between rhizosphere microorganisms and root exudates is useful for exploring the interaction between rhizosphere microorganisms and plants.…”

Section: Introductionmentioning

confidence: 99%

Exogenous Inoculation of Microorganisms Effect on Root Exudates and Rhizosphere Microorganism of Tobaccos

Guo¹,

Yu²,

Sun³

et al. 2021

AiM

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Studying the relationship between rhizosphere microorganisms and root exudates is of great significance for the interaction between rhizosphere microorganisms and plants, and the prevention and control of soil-borne diseases. This article analyzed the effects of different microorganisms on tobacco root exudates and rhizosphere microorganisms. It was found that the bacterial wilt pathogen can greatly increase acids and amines, while the probiotic B. amyloliquefaciens ZM9 can eliminate some acids and amines. The results of the study show that the root exudates of pathogenic bacteria may contain a variety of allelochemicals that cause soil-borne diseases.

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Section: Introductionmentioning

confidence: 99%

Exogenous Inoculation of Microorganisms Effect on Root Exudates and Rhizosphere Microorganism of Tobaccos

Guo¹,

Yu²,

Sun³

et al. 2021

AiM

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“…They also play a role in the root-nodulating plant symbionts (116). In a recent proteomic study by (117), the root exudates of intercropped maize were reported to upregulate the expression of the bacterioferritin comigratory protein in nodule-forming rhizobacterium Ensifer fredii .…”

Section: Discussionmentioning

confidence: 99%

“…In P. aeruginosa, the catabolism of BCAA was shown to contribute to the pathogenesis of the bacterium in Caenorhabditis elegans through manipulation of host energy status and mitochondrial stress signaling potential (129). As oppose to the situation described for P482 and P. aeruginosa, in S. typhimurium and E. fredii exposed to the root exudates of lettuce and intercropped maize, respectively, upregulation of BCAA synthesis rather that their catabolism has been reported (117,125). For some pathogenic bacteria it was shown that a balance between complying to the BCAA nutritional requirement and BCAA starvation supports not only proliferation during infection, but also the evasion of host defenses (130).…”

Section: P482 Cells Treated With Tomato Exudates Switched To Nitric O...mentioning

confidence: 93%

Shared and host-specific transcriptomic response of Pseudomonas donghuensis P482 to the exudates of tomato (Dicot) and maize (Monocot) shed light on the host-adaptive traits in the promiscuous root colonizing bacteria

Krzyżanowska¹,

Jablonska²,

Kaczyński

et al. 2022

Preprint

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Plants of different genotypes and physiological states recruit different populations of root microbiota. The selection is driven by the immune response of the plant and the composition of root exudates. Some bacteria, including Pseudomonas spp., are promiscuous root colonizers. It is yet unclear what particular changes in lifestyle enable them to thrive in the company of different plant hosts. In this study, we used RNAseq to identify genes of the differential (host-specific) and shared (host-independent) transcriptomic responses of a biocontrol strain Pseudomonas donghuensis P482 to the root exudates of two phylogenetically distinct plant species, tomato (Dicot) and maize (Monocot), both of which can be colonized by the bacterium. The host-independent response of P482 to exudates involved upregulated expression of arsenic resistance genes and bacterioferritin synthesis. Contrary, we observed downregulation of pathways related to sulfur assimilation, sensing of ferric citrate and/or other iron carriers, the acquisition of heme, the assembly of the type VI secretion system, and amino acid transport. Pathways upregulated in P482 specifically by tomato exudates included nitric oxide detoxification, repair of iron-sulfur clusters, respiration through the cyanide-insensitive cytochrome bd, and catabolism of amino acids and/or fatty acids. The maize-specific response included upregulation of genes associated with motility, the activity of MexE and two other RND efflux pumps, and copper tolerance. To provide more context to the study, we determined the chemical composition of exudates by GC-MS, NMR, and LC-SRM. Our results bring new insight into the host-driven metabolic adaptations of promiscuous root colonizing bacteria.

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“…phaseoli RBL 1283 was also induced by maize kernel exudates (Hungria et al, 1997). A recent proteomic analysis by Vora et al (2021) showed the expression of NodU protein from Ensifer (Sinorhizobium) fredii NGR234 in response to exudates from maize grown in monoculture and in association with Cajanus cajan. The mechanism by which maize activates the expression of some nod genes is still unknown, but it would be interesting to explore whether, as by legumes, rhizobial gene induction by maize differs among cultivars.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic Responses of Rhizobium phaseoli to Root Exudates Reflect Its Capacity to Colonize Maize and Common Bean in an Intercropping System

et al. 2021

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Corn and common bean have been cultivated together in Mesoamerica for thousands of years in an intercropping system called “milpa,” where the roots are intermingled, favoring the exchange of their microbiota, including symbionts such as rhizobia. In this work, we studied the genomic expression of Rhizobium phaseoli Ch24-10 (by RNA-seq) after a 2-h treatment in the presence of root exudates of maize and bean grown in monoculture and milpa system under hydroponic conditions. In bean exudates, rhizobial genes for nodulation and degradation of aromatic compounds were induced; while in maize, a response of genes for degradation of mucilage and ferulic acid was observed, as well as those for the transport of sugars, dicarboxylic acids and iron. Ch24-10 transcriptomes in milpa resembled those of beans because they both showed high expression of nodulation genes; some genes that were expressed in corn exudates were also induced by the intercropping system, especially those for the degradation of ferulic acid and pectin. Beans grown in milpa system formed nitrogen-fixing nodules similar to monocultured beans; therefore, the presence of maize did not interfere with Rhizobium–bean symbiosis. Genes for the metabolism of sugars and amino acids, flavonoid and phytoalexin tolerance, and a T3SS were expressed in both monocultures and milpa system, which reveals the adaptive capacity of rhizobia to colonize both legumes and cereals. Transcriptional fusions of the putA gene, which participates in proline metabolism, and of a gene encoding a polygalacturonase were used to validate their participation in plant–microbe interactions. We determined the enzymatic activity of carbonic anhydrase whose gene was also overexpressed in response to root exudates.

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Alterations of Primary Metabolites in Root Exudates of Intercropped Cajanus cajan–Zea mays Modulate the Adaptation and Proteome of Ensifer (Sinorhizobium) fredii NGR234

Cited by 13 publications

References 88 publications

Exogenous Inoculation of Microorganisms Effect on Root Exudates and Rhizosphere Microorganism of Tobaccos

Exogenous Inoculation of Microorganisms Effect on Root Exudates and Rhizosphere Microorganism of Tobaccos

Shared and host-specific transcriptomic response of Pseudomonas donghuensis P482 to the exudates of tomato (Dicot) and maize (Monocot) shed light on the host-adaptive traits in the promiscuous root colonizing bacteria

Transcriptomic Responses of Rhizobium phaseoli to Root Exudates Reflect Its Capacity to Colonize Maize and Common Bean in an Intercropping System

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