Alterations of salivary microbial community associated with oropharyngeal and hypopharyngeal squamous cell carcinoma patients

Panda, Mukta; Kumar, Avdhesh; Rahman, Tashnin; Das, Ashok Kumar; Das, Rajjyoti; Sarma, Anupam; Kataki, Amal Ch; Chattopadhyay, Indranil

doi:10.1007/s00203-019-01790-1

Cited by 32 publications

(29 citation statements)

References 85 publications

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“…The overall objective of this study was to determine which oral sites may harbor S. noxia, including saliva and the gingival crevice, but also additional sites, such as the dorsum of the tongue, lingual surfaces of the mandibular incisor, and buccal surface of the maxillary molars. The results of this study were successful in demonstrating that this organism may be detectable most often from samples of unstimulated saliva, comparable to findings reported from other studies [25,26]. These results also support previous reports from this group regarding the oral prevalence of this organism, based upon molecular screening of salivary samples [20,21].…”

Section: Discussionsupporting

confidence: 92%

Microbial Screening Reveals Oral Site-Specific Locations of the Periodontal Pathogen Selenomonas noxia

McDaniel

Samiano

et al. 2021

CIMB

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Introduction: Selenomonas noxia (SN) is an important periodontal pathogen, associated with gingivitis and periodontitis. Many studies have found associations between SN and indicators of poor health outcomes, such as smoking, low socioeconomic status and obesity. However, less is known about the prevalence of this organism and more specifically about other oral site-specific locations that may harbor this organism. Methods: Using an existing patient repository (n = 47) of DNA isolated from saliva and other oral sites (n = 235), including the dorsum of the tongue, lower lingual incisor, upper buccal molar and gingival crevicular fluid (GCF), molecular screening for SN was performed. Screening results were analyzed for associations between demographic variables (age, sex, race/ethnicity) and clinical information (body mass index or BMI, presence of orthodontic brackets, primary/mixed/permanent dentition). Results: qPCR screening revealed a total of n = 62/235 sites or 26.3% harboring SN with saliva and GCF (either alone or in combination with one or more sites) most often observed (Saliva, n = 23/27 or 85.18%, GCF, n = 14/27 or 51%). Analysis of site-specific data revealed most positive results were found among saliva and GCF alone or in combination, with fewer positive results observed among the tongue (33.3%), lower lingual incisor (29.6%), and upper buccal molar (25.9%). No significant associations were found between demographic or clinical variables and presence of SN at any site. Conclusions: These results may be among the first to describe site-specific locations of S. noxia among various additional oral biofilm sites. These data may represent a significant advancement in our understanding of the sites and locations that harbor this organism, which may be important for our understanding of the prevalence and distribution of these organisms among patients of different ages undergoing different types of oral treatments, such as orthodontic treatment or therapy.

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Section: Discussionsupporting

confidence: 92%

Microbial Screening Reveals Oral Site-Specific Locations of the Periodontal Pathogen Selenomonas noxia

McDaniel

Samiano

et al. 2021

CIMB

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“…The characteristics of the selected studies are described in detail in Table 1 . Of the twenty-six studies, ten were from the United States of America [ 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 ], four were from China [ 22 , 23 , 24 , 25 ], three were from Taiwan [ 7 , 26 , 27 ], two were from India [ 28 , 29 ], and others were from Yemen [ 30 ], Malaysia [ 31 ], Australia [ 32 ], Japan [ 33 , 34 ], Sri Lanka [ 35 ], and New Zealand [ 36 ]. The study design for all the studies was cross-sectional, except for one which employed a prospective methodology [ 19 ].…”

Section: Resultsmentioning

confidence: 99%

“…The majority of the studies included both genders as the subjects except two studies which were restricted to male subjects only [ 12 , 35 ]. Other associated factors such as cigarette smoking, betel quid chewing, and alcohol use, HPV, etc., were investigated in fifteen of the selected studies [ 7 , 12 , 20 , 21 , 23 , 24 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 34 , 35 ]. The ability to compare or incorporate the results of individual studies was restricted by the extensive differences in various aspects of the studies.…”

Section: Resultsmentioning

confidence: 99%

Association of Microbiome with Oral Squamous Cell Carcinoma: A Systematic Review of the Metagenomic Studies

Mun

Lum

Sze

et al. 2021

IJERPH

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The past decade has witnessed a surge in epidemiological studies that have explored the relationship between the oral microbiome and oral cancer. Owing to the diversity of the published data, a comprehensive systematic overview of the currently available evidence is critical. This review summarises the current evidence on the metagenomic studies on the oral microbiome in oral cancer. A systematic search was conducted in Medline and Embase databases to identify original studies examining the differences in the oral microbiome of oral cancer cases and controls. A total of twenty-six studies were identified that reported differences in microbial abundance between oral squamous cell carcinoma (OSCC) and controls. Although almost all the studies identified microbial dysbiosis to be associated with oral cancer, the detailed qualitative analysis did not reveal the presence/abundance of any individual bacteria or a consortium to be consistently enriched in OSCC samples across the studies. Interestingly, few studies reported a surge of periodontopathogenic taxa, especially Fusobacteria, whereas others demonstrated a depletion of commensal taxa Streptococci. Considerable heterogeneity could be identified in the parameters used for designing the studies as well as reporting the microbial data. If microbiome data needs to be translated in the future, to complement the clinical parameters for diagnosis and prognosis of oral cancer, further studies with the integration of clinical variables, adequate statistical power, reproducible methods, and models are required.

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“…Saliva is a biological fluid with features such as ease of sampling, cost-effectiveness, and non-invasiveness that make it a perfect diagnostic specimen, highlighting the importance of developing a method for early detection of OSCC based on validated salivary biomarkers [ 14 , 15 , 16 , 17 , 18 ]. Several well-designed studies recently reported on potential detection methods for oral cancer, suggesting analysis of salivary RNA and proteins [ 19 , 20 ], microRNAs [ 21 , 22 ], metabolites [ 23 , 24 ], glycoprotein [ 25 , 26 , 27 , 28 , 29 ], and the microbiome [ 30 , 31 , 32 , 33 , 34 , 35 , 36 ]. However, as yet, no suitable biomarkers are regularly used in clinical practice for the management of oral cancer.…”

Section: Introductionmentioning

confidence: 99%

Verification of Saliva Matrix Metalloproteinase-1 as a Strong Diagnostic Marker of Oral Cavity Cancer

Chang

Chu

Liu

et al. 2020

Cancers

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Oral squamous cell carcinoma (OSCC) accounts for >90% of cases of oral cancer, including cancer at the lip and oral cavity and cancer at the oropharynx. Most OSCCs develop from oral potentially malignant disorders (OPMDs), which consist of heterogeneous lesions with different malignant transformation potentials that make early detection of OSCC a challenge. Using a targeted mass spectrometry-based assay to compare multiple candidate proteins, we previously identified matrix metalloproteinase-1 (MMP-1) as one of the most promising salivary OSCC biomarkers. To explore the clinical utility of MMP-1 in OSCC detection, we developed an in-house, sensitive enzyme-linked immunosorbent assay (ELISA) for measuring MMP-1 content, and tested it on saliva samples from 1160 subjects (313 healthy controls, and 578 OPMD and 269 OSCC patients) collected at two medical centers. Salivary MMP-1 levels measured by our in-house ELISA significantly discriminated OSCC patients from non-cancerous groups. A receiver operating characteristic curve analysis showed that MMP-1 was effective in separating non-cancer groups from patients with OSCCs at the oral cavity. Additionally, salivary MMP-1 levels in oral cavity cancer patients were highly correlated with tumor progression (tumor size, lymph node metastasis, and overall stage). Collectively, our results indicate that salivary MMP-1 is an effective biomarker for OSCC that can be sensitively detected using our newly developed ELISA. The newly developed MMP-1 ELISA may be used as a new adjunctive tool to aid in detecting and monitoring OSCC.

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Alterations of salivary microbial community associated with oropharyngeal and hypopharyngeal squamous cell carcinoma patients

Cited by 32 publications

References 85 publications

Microbial Screening Reveals Oral Site-Specific Locations of the Periodontal Pathogen Selenomonas noxia

Microbial Screening Reveals Oral Site-Specific Locations of the Periodontal Pathogen Selenomonas noxia

Association of Microbiome with Oral Squamous Cell Carcinoma: A Systematic Review of the Metagenomic Studies

Verification of Saliva Matrix Metalloproteinase-1 as a Strong Diagnostic Marker of Oral Cavity Cancer

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