Alterations of the gut microbiome of largemouth bronze gudgeon (Coreius guichenoti) suffering from furunculosis

Abstract

High-throughput sequencing was applied to compare the intestinal microbiota in largemouth bronze gudgeon either healthy or affected by furunculosis. Proteobacteria, Actinobacteria, Tenericutes, Firmicutes and Bacteroidetes were detected as the predominant bacterial phyla in the gut of both diseased and healthy fish. The abundance of Proteobacteria differed significantly between the two groups of fish, mainly due to the overwhelming prevalence of Aeromonas in the diseased fish (81% ± 17%), while the genus was unevenly spread among the apparently healthy fish (33% ± 33%). The bacterial diversity in the intestine of diseased fish was markedly lower than in healthy fish. Analysis revealed the significant dissimilarity between the gut microbiota of diseased and healthy fish. The bacterial profiles in the gut were further characterized with the 28 phylotypes that were shared by the two groups. In diseased fish, two shared OTUs (OTU0001 and OTU0013) were closely related to Aeromonas salmonicida, their total proportion exceeding 70% of the sequences in diseased fish, while averaging 5.2% ± 4.6% in the healthy fish. This result suggested the presence of healthy carriers of pathogenic A. salmonicida among the farmed fish, and the gut appeared as a probable infection source for furunculosis in largemouth bronze gudgeon.

Effect of tributyrin supplementation in high‐soya bean meal diet on growth performance, body composition, intestine morphology and microbiota of juvenile yellow drum (Nibea albiflora)

“…In humans, reduced microbial diversity is associated with diseases such as obesity, type 2 diabetes (Brunak et al, ) and bowel disease (Shin, Whon, & Bae, ). Similarity, the bacterial diversity was markedly lower than in healthy fish (Li et al, ). The increase in microbial diversity in fish fed tributyrin supplementation of high‐SBM diet could be considered as reversing signs of intestine health.…”

“…PCoA showed that intestine content samples from fish that followed the same diet clustered together, although the SBM diet showed greater dispersion. As about the taxonomy community, no statistic difference in Proteobacteria relative abundance was observed among groups at phylum level, although imbalanced intestine microbiota often arises from a sustained increase in abundance of the phylum Proteobacteria (Li et al, ). However, within Proteobacteria, the SBM diet favoured the dominance of Acinetobacter over Brevundimonas , indicating the role of tributyrin in the regulation of intestine microbial composition.…”

Progress and perspectives of short‐chain fatty acids in aquaculture

“…) as well as in largemouth bronze gudgeon ( Coreius guichenoti ) and grass carp, which suffer from infections (Li et al . ; Tran et al . ), resulted in significant changes in gut microbiota.…”

“…A recent study revealed that gut dysbiosis in Tsumura Suzuki obese diabetes (TSOD) mice leads to a reduction in total plasma SCFA levels, where acetate was decreased, with an increase in propionate and butyrate, while valerate and hexanoate were absent in the TSOD mice compared with controls (Nishitsuji et al 2017). In aquatic animals such as Atlantic salmon (Salmo salar) and Atlantic cod (Gadus morhua), supplementation with soybean-derived protein (Ringø et al 2006;Green et al 2013) as well as in largemouth bronze gudgeon (Coreius guichenoti) and grass carp, which suffer from infections (Li et al 2016;Tran et al 2018), resulted in significant changes in gut microbiota. It is unclear whether the changes of microbiota structure in these studies directly affected the levels of SCFA in the host gut.…”

Using fish models to investigate the links between microbiome and social behaviour: The next step for translational microbiome research?

“…Disentangling the links between microbiome and fish health is a hot topic, particularly given the growth of the aquaculture industry. In this respect, the effects of pathogens, antibiotics and probiotics on microbiome composition have been subject of several studies showing that significant changes take place (e.g., Carlson, Leonard, Hyde, Petrosino, & Primm, 2017;Gonçalves & Gallardo-Escarate, 2017;Hennersdorf et al, 2016;Li et al, 2016;Llewellyn et al, 2017;Reid et al, 2017).…”