Altered anxiety and social behaviors in a mouse model of Fragile X syndrome treated with hyperbaric oxygen therapy

Luo, Chao-Wen; Deng, Xiaoyan; Cheng, Jin-Ling; Xiao, Dan-Xian; Zhang, Chuyu; Feng, Jiaxin; Chen, Shengqiang; Hu, Nan

doi:10.1016/j.jocn.2020.02.004

Cited by 4 publications

(10 citation statements)

References 27 publications

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“…However, there is limited research published on how to evaluate this kind of behaviour of the Fmr1 KO mouse. Only 1 research has conducted on this topic and the result of this article showed that the Fmr1 KO mouse demonstrated a longer time and increased frequency with the familiar mouse than the sphere [22], which is consistent with our result. This nding may allude that there is a pre-existing preference in the Fmr1 KO mouse to prefer social behaviour rather than non-social behaviour.…”

Section: Discussionsupporting

confidence: 91%

“…In the Elevated Plus-Maze test, the behaviour presenting more entrances into the open area with prolonged staying time and more distances travelled would be normally considered as lower in anxiety [22]. When comparing to the normal WT mouse, Fmr1 KO mouse demonstrated increasing activities at the open area proven by more staying time, the number of entrances and distances travelled [26].…”

Section: Discussionmentioning

confidence: 99%

“…The data collection period would last for 5 minutes each time. The experiment method was drawn from related literature listed in the References section [17,21,22,23].…”

Section: Open Field Testmentioning

confidence: 99%

“…A camera would record the trajectory of the subjects and the app SMART was used to record the frequency, distance travelled and duration of staying time for 5 minutes. The experiment method was drawn from related literature listed in the References section [21,22,23].…”

Section: Elevated Plus-maze Testmentioning

confidence: 99%

“…At each phase, the camera would record the trajectory of the subject mouse and the app SMART would be used to record the frequency and duration of time spent at each cage (the contacting area would be 4.0 cm around the cage) for 10 minutes. The experiment method was drawn from related literature listed in the References section [17,22,23,24].…”

Section: Three-box Social Interaction Testmentioning

confidence: 99%

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Altered Behaviour Associated With Autism in a Mouse Model of Fragile X Syndrome Treated With Bacteroides Fragilis BF839

Lin

Zeng

Lin

et al. 2020

Preprint

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Background: Fragile X syndrome (FXS), tightly related to the morbidity of Autism spectrum disorder (ASD), is a common hereditary syndrome often associated with retardation of intelligence. Some key symptoms of ASD such as anxiety, cognitive impairment and social anxiety disorder are also the predominant features in FXS. Children with ASD are often performed with gastrointestinal symptoms. According to the existing research, with the treatment with Bacteroides Fragilis BF839, mice with ASD will have better performance in communication and social behaviours with less anxiety and perceptual disorder. In this article, we have observed the impact of Bacteroides Fragilis BF839, a well-established Chinese bacteria strain with the human intestine origin, on mice with FXS and their behavioural disorders accordingly. Result: Based on the Open Field test, compared to the Fmr1KO group, mice treated with BF839 showed prolonged staying time in the center of the container. This finding suggests that BF839 can improve Fmr1KO mice's self-exploration behaviour and dented their anxiety. The Elevated Plus Maze test indicated BF839 treated mice presented more activities in entering open arms, prolonged time of staying and significantly less distance travelled at the plus-maze, along with less entering behaviours in the closed arms with less time of staying and more distance travelled. This result proved that with the treatment of BF839, Fmr1KO mice have improved ability in recognizing the surrounding environment and greater senses at detecting danger. Three-box Social Interaction test confirmed that BF839 strengthens the social novelty preference of the Fmr1KO mice, proven by their increasing duration and frequency in social interacting with the stranger mouse. The final experiment named the Pool Maze test presented the result that on the fourth day, BF839 treated mice have shown significantly shortened escape latency. Meanwhile, on Day 5, BF839 treated group performed increasing frequency in passing through the platform, which, along with the shortened escape latency, demonstrated BF839 has the function of improving Fmr1KO mice's cognitive capacity and their ability to extract information from the surrounding environment.Conclusion: Based on the outcome of each test performed, Bacteroides Fragilis BF839 can successfully improve Autism related abnormal behaviours in mice with FXS. Bacteroides Fragilis BF839 can be a potential intervention strategy in treating FXS and ASD safely and effectively.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

“…The data collection period would last for 5 minutes each time. The experiment method was drawn from related literature listed in the References section [17,21,22,23].…”

Section: Open Field Testmentioning

confidence: 99%

Section: Elevated Plus-maze Testmentioning

confidence: 99%

Section: Three-box Social Interaction Testmentioning

confidence: 99%

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Altered Behaviour Associated With Autism in a Mouse Model of Fragile X Syndrome Treated With Bacteroides Fragilis BF839

Lin

Zeng

Lin

et al. 2020

Preprint

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The interplay of hypoxic and mental stress: Implications for anxiety and depressive disorders

Burtscher¹,

Niedermeier²,

Hüfner³

et al. 2022

Neuroscience & Biobehavioral Reviews

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Effects of hyperbaric oxygen therapy on autistic behaviors and GRIN2B gene expression in valproic acid-exposed rats

Mohtaj Khorassani,

Moghimi,

Khakzad

et al. 2024

Front. Neurosci.

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IntroductionAutism is a complex neurodevelopmental condition characterized by deficits in social interaction, communication, and restricted repetitive behaviors. Hyperbaric oxygen therapy (HBOT) has emerged as a potential treatment for autism, although its effects on behavior and gene expression are not well understood. The GRIN2B gene, known for its involvement in encoding a glutamate receptor subunit crucial for neuron communication and associated with autism, was a focus of this study.MethodsUsing a rat model induced by prenatal exposure to valproic acid, we examined the impact of HBOT on autism-like behaviors and GRIN2B gene expression. Male Wistar rats were categorized into four groups: control, VPA (valproic acid-exposed), VPA+HBOT [2 atmosphere absolute (ATA)], and VPA+HBOT (2.5 ATA). The rats underwent several behavioral tests to assess social behavior, anxiety, stereotype and exploratory behaviors, and learning. Following the behavioral tests, the HBOT groups received 15 sessions of HBOT at pressures of 2 and 2.5 (ATA), and their behaviors were re-evaluated. Subsequently, real-time PCR was employed to measure GRIN2B gene expression in the frontal lobe.ResultsOur results indicated that HBOT significantly increased social interaction and exploratory behaviors in VPA-exposed rats, alongside elevated GRIN2B gene expression in their frontal lobe.DiscussionOur findings imply that HBOT might have a potential role in ameliorating autism-related behaviors in the VPA rat model of autism through potential modulation of GRIN2B gene expression. However, additional research is essential to fully comprehend the underlying mechanisms and refine the HBOT protocol for optimizing its effectiveness in improving autism-related symptoms.

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Altered anxiety and social behaviors in a mouse model of Fragile X syndrome treated with hyperbaric oxygen therapy

Cited by 4 publications

References 27 publications

Altered Behaviour Associated With Autism in a Mouse Model of Fragile X Syndrome Treated With Bacteroides Fragilis BF839

Altered Behaviour Associated With Autism in a Mouse Model of Fragile X Syndrome Treated With Bacteroides Fragilis BF839

The interplay of hypoxic and mental stress: Implications for anxiety and depressive disorders

Effects of hyperbaric oxygen therapy on autistic behaviors and GRIN2B gene expression in valproic acid-exposed rats

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