Altered contractile properties of rat cardiac muscle during experimental thiamine deficiency and food deprivation

Cappelli, V.; Bottinelli, Roberto; Polla, Biagio; Reggiani, Carlo

doi:10.1016/0022-2828(90)90073-b

Cited by 35 publications

(22 citation statements)

References 32 publications

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“…Figure 2 illustrates the gain of body weight during the 2 wk of thiamine deprivation in the three rat strains. No changes in the body weight were observed as a result of thiamine-deficient treatment in the WKY/ NCrj and SHR/Izm rats, a finding that is consistent with previous studies using rodent models (11,27). By contrast, throughout the 2-wk treatment, a thiaminedeficient chow diet resulted in an increased body weight in the SHR/NCrj rats, compared with those with normal rat chow.…”

Section: Resultssupporting

confidence: 76%

“…The intake of chow was significantly decreased in thiamine-deprived rats 3 days after the commencement of treatment, a finding that was consistent with previous studies that used rodent models (11,27). Nevertheless, the body weight increased significantly in thiamine-deficient rats compared with normal rats.…”

Section: Effects Of Thiamine Deficiency and Resupplementation On Bodysupporting

confidence: 81%

“…Thiamine deprivation for 2 wk, which was rather shorter than the past studies, e.g., 5 wk in past studies (11,27), brought about distinct results in CD36-defect rats (SHR/NCrj) compared with CD36-nondefect rats (WKY/NCrj and SHR/Izm). The experimental period of 2 wk was selected because a state of severe thiamine depletion emerged on a strict thiamine-deficient diet for 18 days (31).…”

Section: Discussionmentioning

confidence: 93%

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Gene-environment interactions in wet beriberi: effects of thiamine depletion in CD36-defect rats

Tanaka

Kono

Terasaki

et al. 2003

American Journal of Physiology-Heart and Circulatory Physiology

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sushi Kitaura. Gene-environment interactions in wet beriberi: effects of thiamine depletion in CD36-defect rats. Am J Physiol Heart Circ Physiol 285: H1546-H1553, 2003; 10.1152/ajpheart.00182.2003.-Selective vulnerability to thiamine deficiency is known to occur between individuals and within different tissues. However, no comprehensive explanation for this has been found, and there are no reports that reproduce the cardiovascular manifestations of human wet beriberi in animals. We hypothesized that the distinction of substrate reliance, namely, the primary dependency on glucose as substrate, could be an underlying factor in the selective vulnerability of thiamine deficiency. In the setting of impaired fatty acid entry, which occurs in CD36-defect rats, substrate reliance shifts from fatty acid to glucose, which would be expected to lead to a susceptibility to thiamine deficiency. Genomic DNA was analyzed for CD36 defects in three cognate strains of rats [spontaneously hypertensive rats (SHR)/NCrj, SHR/Izm, and Wistar-Kyoto (WKY)/NCrj], which identified the presence of a CD36 defect in SHR/NCrj rats but not in SHR/Izm and WKY/NCrj rats. Treatment with 2 wk of thiamine-depleted chow on 4-wk-old rats of each of these strains resulted in increased body and lung weight in the SHR/NCrj rats but not in the SHR/Izm and WKY/NCrj rats. The increased lung weight in the SHR/NCrj rats was accompanied with histological changes of congestive vasculopathy, which were not observed in either the SHR/Izm or the WKY/NCrj rats. Thiamine-deficient 12-wkold SHR/NCrj rats demonstrated increased body weight (305.6 Ϯ 6.2 g in thiamine-deficient rats vs. 280.8 Ϯ 9.1 g in control; P Ͻ 0.0001), lactic acidemia (pH, 7.322 Ϯ 0.026 in thiamine-deficient rats vs. 7.443 Ϯ 0.016 in control; P Ͻ 0.0001; lactate, 2.42 Ϯ 0.28 mM in thiamine-deficient rats vs.

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Section: Resultssupporting

confidence: 76%

Section: Effects Of Thiamine Deficiency and Resupplementation On Bodysupporting

confidence: 81%

Section: Discussionmentioning

confidence: 93%

See 1 more Smart Citation

Gene-environment interactions in wet beriberi: effects of thiamine depletion in CD36-defect rats

Tanaka

Kono

Terasaki

et al. 2003

American Journal of Physiology-Heart and Circulatory Physiology

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“…However, very little is known about the effects of thiamine deficiency on the myocardium (5). Cappelli et al (5) have shown evidence that supports the view that cardiac contractility is deeply affected in the rat by thiamine deficiency. Thiamine is also considered a clinically important factor in heart function, and its deficiency has been reported to cause heart failure (28,33,35).…”

mentioning

confidence: 75%

Abolition of reperfusion-induced arrhythmias in hearts from thiamine-deficient rats

Oliveira¹,

Guatimosim²,

Castro³

et al. 2007

American Journal of Physiology-Heart and Circulatory Physiology

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tensive work has been done regarding the impact of thiamine deprivation on the nervous system. In cardiac tissue, chronic thiamine deficiency is described to cause changes in the myocardium that can be associated with arrhythmias. However, compared with the brain, very little is known about the effects of thiamine deficiency on the heart. Thus this study was undertaken to explore whether thiamine deprivation has a role in cardiac arrhythmogenesis. We examined hearts isolated from thiamine-deprived and control rats. We measured heart rate, diastolic and systolic tension, and contraction and relaxation rates. Whole cell voltage clamp was performed in rat isolated cardiac myocytes to measure L-type Ca 2ϩ current. In addition, we investigated the global intracellular calcium transients by using confocal microscopy in the line-scan mode. The hearts from thiaminedeficient rats did not degenerate into ventricular fibrillation during 30 min of reperfusion after 15 min of coronary occlusion. The antiarrhythmogenic effects were characterized by the arrhythmia severity index. Our results suggest that hearts from thiamine-deficient rats did not experience irreversible arrhythmias. There was no change in L-type Ca 2ϩ current density. Inactivation kinetics of this current in Ca 2ϩ -buffered cells was retarded in thiamine-deficient cardiac myocytes. The global Ca 2ϩ release was significantly reduced in thiaminedeficient cardiac myocytes. The amplitude of caffeine-releasable Ca 2ϩ was lower in thiamine-deficient myocytes. In summary, we have found that thiamine deprivation attenuates the incidence and severity of postischemic arrhythmias, possibly through a mechanism involving a decrease in global Ca 2ϩ release.arrhythmia; calcium current; confocal microscopy THIAMINE DEFICIENCY CAUSES cardiovascular and neurological damage that presents clinically as beriberi. Thiamine pyrophosphate is an important cofactor in several vital enzymatic reactions involved in metabolism and energy production (34, 39). Following its uptake by the cell, thiamine rapidly undergoes pyrophosphorylation, yielding thiamine pyrophosphate, which is the active substance. Extensive work has been done regarding the impact of thiamine deprivation on the nervous system. However, very little is known about the effects of thiamine deficiency on the myocardium (5). Cappelli et al. (5) have shown evidence that supports the view that cardiac contractility is deeply affected in the rat by thiamine deficiency. Thiamine is also considered a clinically important factor in heart function, and its deficiency has been reported to cause heart failure (28, 33, 35). The general view that reperfusion of the ischemic myocardium exerts a beneficial effect by preventing the ischemiainduced changes in cardiac performance has been supported by a number of studies; however, reperfusion after a certain critical time has been shown to be deleterious to cardiac function (16). Intracellular Ca 2ϩ has an important role in heart function, and studies have been conducted to investigate pos...

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“…From 21 days to 10 wk old, the rats were housed in individual wire cages with raised wire floors to avoid coprophagy (4,7). Dietary restriction was maintained by limiting the amount of chow (Purina Labina, Sã o Paulo, Brazil) provided to the UG rats to 50% of that consumed by the age-matched control rats.…”

Section: Pcm Two-phase Induction Protocolmentioning

confidence: 99%

Ventricular remodeling and diastolic myocardial dysfunction in rats submitted to protein-calorie malnutrition

Fioretto

Querioz

Padovani

et al. 2002

American Journal of Physiology-Heart and Circulatory Physiology

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Altered contractile properties of rat cardiac muscle during experimental thiamine deficiency and food deprivation

Cited by 35 publications

References 32 publications

Gene-environment interactions in wet beriberi: effects of thiamine depletion in CD36-defect rats

Gene-environment interactions in wet beriberi: effects of thiamine depletion in CD36-defect rats

Abolition of reperfusion-induced arrhythmias in hearts from thiamine-deficient rats

Ventricular remodeling and diastolic myocardial dysfunction in rats submitted to protein-calorie malnutrition

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