Altered Cytoskeletal Structure of Smooth Muscle Cells in Ureteropelvic Junction Obstruction

Cutroneo, Giuseppina; Arena, Salvatore; Anastasi, Giuseppe; Cervellione, Raimondo M.; Grimaldi, Silvia; Mauro, Debora Di; Speciale, Francesco; Arena, Francesco; Benedetto, Vincenzo Di; Favaloro, Angelo; Magno, Carlo

doi:10.1016/j.juro.2011.02.045

Cited by 22 publications

(17 citation statements)

References 14 publications

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“…Similarly, it has been observed that muscular cells in congenital obstructed uretero-pelvic junction in humans are in an embryonic state as well. In particular, a higher expression of embryonic α7A and β1A isoforms and a lower expression of adult subunit α7B and β1D isoforms were documented in the muscular cells of congenital obstructed uretero-pelvic junction as compared to control specimens [10]. In our study, we tried to investigate the expression of embryonic α7A and β1A and mature α7B and β1D integrins in cremaster muscular cells of patients affected by a congenital undescended testis.…”

Section: Discussionmentioning

confidence: 94%

“…Previous findings reported a critical structural impairment of the cytoskeleton at the level of the congenital obstructed uretero-pelvic junction, linked to an abnormal arrangement of integrins [10].…”

Section: Introductionmentioning

confidence: 92%

“…The α7A isoform, in particular, has been reported to be highly expressed in immature myogenic cells and it is later replaced by α7B in mature muscle cells. Similarly, β1A is highly expressed in proliferating myogenic precursor cells and it is then substituted by β1D in mature muscle cells [10].…”

Section: Introductionmentioning

confidence: 99%

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Altered Integrins Expression of Patients Affected by Cryptorchidism

et al. 2018

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Objectives: The study aimed to investigate the expression of the integrin isoforms α7A and β1A, expressed by myogenic precursor cells, and α7B and β1D, expressed by mature muscle cells in the cremaster of patients affected by an undescended testis. Methods: Fifteen samples of cremaster were obtained from patients undergoing surgery for an undescended testis. Thirty control specimens of cremaster were harvested from patients with congenital hydrocele or inguinal hernia. Immunofluorescent analysis was carried out using anti-α7A, β1A, α7B, and β1D integrin antibodies. Sections were observed using confocal laser scanning microscopy. Results: As compared with controls, a significant loss of a α7B (p = 0.0355) and β1D (p = 0.0069) integrins and a higher expression of α7A (p = 0.0003) and β1A (p = 0.0150) was detected in the cremaster of patients affected by an undescended testis. Conclusions: Our data document a critical alteration of the cytoskeleton of cremasteric smooth muscle cells in patients with an undescended testis. This might explain the altered function in smooth muscle cells in cremaster implied during testicular descent. We therefore speculate that the postnatal splicing of α7A to α7B and of β1A to β1D integrins is delayed. This could account for the common clinical scenario of spontaneous descent of the testes in the first months of life.

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Section: Discussionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 92%

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Altered Integrins Expression of Patients Affected by Cryptorchidism

et al. 2018

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“…Öte yandan kronik fazda ise, hidronefroz, renal atrofi, böbrek ağırlığında azalma, intertisyel fibrozis ve böbrek fonksiyon bozukluğu gelişmektedir (24) . Deneysel ve klinik çalışmalar (1,3,6,10,16,27,30) ; nefropatiye neden olan başlıca faktörle-rin glomerüler dinamikleri ve tübüler fonksiyonları etkileyen çeşitli vazoaktif peptidler ve bunların neden olduğu apopitotik mekanizmalar olduğunu gös-termiştir.…”

Section: Discussionunclassified

The early and late period alterations on the contralateral renal parenchyma secondary to unilateral ureteropelvic junction obstruction: experimental study

Kaçar¹,

Çetin²,

Çalışkan³

et al. 2010

TCCD

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“…Kaya et al showed an overexpression of extracellular matrix proteins and decreased nerve supply in intrinsic UPJ obstruction (3). Cutroneo et al demonstrated an increasing activation of apoptosis and a critical alteration in UPJ obstruction (4).…”

mentioning

confidence: 99%

Analysis of expression of TNF-alpha and TGF-beta3 in intrinsic ureteropelvic junction obstruction

Koca¹,

Kaya²,

Mi³

et al. 2013

BLL

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Abstract:Objectives: Ureteropelvic junction (UPJ) obstruction is of critical importance to understand the histopathology of UPJ obstruction in terms of therapy planning and follow-up. For this purpose, our study was conducted with TNF-α and TGF-β markers to investigate possible underlying problems in intrinsic UPJ obstruction. Methods: Of the patients who had undergone surgery in our clinic, 36 UPJ segments of patients who had undergone dismembered pyeloplasty surgery due to UPJ obstruction and 14 UPJ segments of the patients who had undergone nephrectomy were collected to form 2 groups. All histological sections were examined by applying immunohistochemical transforming growth factor beta 3 (TGF-β3) and tumour necrosis factor alpha (TNF-α) monoclonal antibody dyes. Results: The mean staining values for TNF-α in mucosal tissue and mucosa were 0.53±0.84 and 0.58±0.84, respectively in the obstruction group, whereas the values observed in the control group were 0.86±0.36 and 0.93±0.47, respectively. While the mean staining values in the obstruction group in mucosal tissue and mucosa for TGF-β3 were 1.75±0.73 and 2.17±0.77, respectively, the values established in the control group were 1.14±0.66 and 1.43±0.93, respectively. The difference between the obstruction and control groups were statistically signifi cant for both values (p<0.05). Conclusion: Only a limited number of studies have been carried out on this particular issue. Data from the present study indicate that TGF-β3 and TNF-α may play a role in the histopathogenesis of UPJ obstruction (Tab. 1, Fig. 1

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Altered Cytoskeletal Structure of Smooth Muscle Cells in Ureteropelvic Junction Obstruction

Cited by 22 publications

References 14 publications

Altered Integrins Expression of Patients Affected by Cryptorchidism

Altered Integrins Expression of Patients Affected by Cryptorchidism

The early and late period alterations on the contralateral renal parenchyma secondary to unilateral ureteropelvic junction obstruction: experimental study

Analysis of expression of TNF-alpha and TGF-beta3 in intrinsic ureteropelvic junction obstruction

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