Altered expression of Krüppel-like factor 4 and β-catenin in human gastric cancer

Zhang, Neng; Zhang, Jun; Wang, Ziwei; Zha, Lang; Huang, Zhen

doi:10.3892/ol.2012.619

Cited by 17 publications

(14 citation statements)

References 39 publications

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“…The KLF4 protein contains both activator and repressor domains, and can function as a positive or negative regulator of gene expression [27-29]. Oncogenic effects of KLF4 have been reported in breast, skin, and lung cancers [14,15,30].…”

Section: Discussionmentioning

confidence: 99%

A role for low-abundance miRNAs in colon cancer: the miR-206/Krüppel-like factor 4 (KLF4) axis

et al. 2012

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BackgroundMicroRNAs (miRNAs or miRs) are short non-coding RNAs that affect the expression of genes involved in normal physiology, but that also become dysregulated in cancer development. In the latter context, studies to date have focused on high-abundance miRNAs and their targets. We hypothesized that among the pool of low-abundance miRNAs are some with the potential to impact crucial oncogenic signaling networks in colon cancer.ResultsUnbiased screening of over 650 miRNAs identified miR-206, a low-abundance miRNA, as the most significantly altered miRNA in carcinogen-induced rat colon tumors. Computational modeling highlighted the stem-cell marker Krüppel-like factor 4 (KLF4) as a potential target of miR-206. In a panel of primary human colon cancers, target validation at the mRNA and protein level confirmed a significant inverse relationship between miR-206 and KLF4, which was further supported by miR-206 knockdown and ectopic upregulation in human colon cancer cells. Forced expression of miR-206 resulted in significantly increased cell proliferation kinetics, as revealed by real-time monitoring using HCT116 cells.ConclusionsEvolutionarily conserved high-abundance miRNAs are becoming established as key players in the etiology of human cancers. However, low-abundance miRNAs, such as miR-206, are often among the most significantly upregulated miRNAs relative to their expression in normal non-transformed tissues. Low-abundance miRNAs are worthy of further investigation, because their targets include KLF4 and other pluripotency and cancer stem-cell factors.

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Section: Discussionmentioning

confidence: 99%

A role for low-abundance miRNAs in colon cancer: the miR-206/Krüppel-like factor 4 (KLF4) axis

et al. 2012

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“…Therefore, it is necessary to screen more new biomarkers. Our recent studies have reported several molecules, such as KLF4 (Krüppel-like factor 4) (Zhang et al, 2012) and HMGA2 (high mobility group protein A2) (Zha et al, 2013), correlate with GC progression and prognosis. In present study, our data suggested that AMFR was a new candidate prognostic marker for GCs.…”

Section: Discussionmentioning

confidence: 99%

Aberrant Expression of the Autocrine Motility Factor Receptor Correlates with Poor Prognosis and Promotes Metastasis in Gastric Carcinoma

Huang

Zhang²,

Zha³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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AMFR, autocrine motility factor receptor, also called gp78, is a cell surface cytokine receptor which has a dual role as an E3 ubiquitin ligase in endoplasmic reticulum-associated degradation. AMFR expression is associated with tumor malignancy. We here investigated the clinical significance of AMFR and its role in metastasis and prognosis in gastric cancer. Expression of AMFR, E-cadherin and N-cadherin in cancer tissues and matched adjacent normal tissues from 122 gastric cancer (GC) patients undergoing surgical resection was assessed by immunohistochemistry. Levels of these molecules in 17 cases selected randomly were also analysed by Western blotting. AMFR expression was significantly increased in gastric cancer tissues, and associated with invasion depth and lymph node metastasis. Kaplan-Meier analysis showed AMFR expression correlated with poor overall survival and an increased risk of recurrence in the GC cases. Cox regression analysis suggested AMFR to be an independent predictor for overall and recurrence-free survival. E-cadherin expression was decreased in gastric cancer tissues; conversely, N-cadherin was increased. Expression of AMFR negatively correlated with E-cadherin expression, whereas N-cadherin expression showed a significant positive correlation with AMFR expression. AMFR might be involved in the regulation of epithelial-mesenchymal transition, with aberrant expression correlating with a poor prognosis and promoting invasion and metastasis in GCs.

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“…KLF-4 is highly expressed in adult guts (Zheng et al, 2009) and act by regulating intestinal differentiation (Li et al, 2011). Recently, the tumor suppressive role of KLF-4 has been identified in various cancers, including gastric cancer (Zhang et al, 2012), prostate cancer (Shin et al, 2014), esophageal cancer (Zhang et al, 2009), and lung cancer (Zhou et al, 2010). KLF-4 acts by deregulating the malignant transformation and cellular hyperproliferation.…”

Section: Introductionmentioning

confidence: 99%

Tumor-suppressive role of Kruppel-like factor 4 (KLF-4) in colorectal cancer

et al. 2017

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ABSTRACT. Kruppel-like factors (KLFs) are a group of transcriptional regulators that have recently been identified to exhibit tumorsuppressive function against various gastrointestinal cancers. The present study aims to investigate the expression patterns and prognostic value of KLF-4 in colorectal cancers (CRCs). KLF-4 levels in CRC tissues were examined via immunohistochemistry analysis, real-time quantitative polymerase chain reaction, and western blotting. The chisquare test was performed to evaluate the correlation between KLF-4 expression and the clinicopathological characteristics. Kaplan-Meier analysis was performed to assess the prognostic value of KLF-4 in CRC patients. In addition, we evaluated the effect of KLF-4 knockdown on the proliferation of CRC HT-29 cells. Our results showed significant downregulation of KLF-4 in 31 CRC samples, collected from CRC patients showing more malignant characteristics such as lymphatic metastasis, low tumor cell differentiation, and tumor recurrence. CRC patients in the low KLF-4 group were found to have reduced overall

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Altered expression of Krüppel-like factor 4 and β-catenin in human gastric cancer

Cited by 17 publications

References 39 publications

A role for low-abundance miRNAs in colon cancer: the miR-206/Krüppel-like factor 4 (KLF4) axis

A role for low-abundance miRNAs in colon cancer: the miR-206/Krüppel-like factor 4 (KLF4) axis

Aberrant Expression of the Autocrine Motility Factor Receptor Correlates with Poor Prognosis and Promotes Metastasis in Gastric Carcinoma

Tumor-suppressive role of Kruppel-like factor 4 (KLF-4) in colorectal cancer

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