Altered expression of microRNA-451 in eutopic endometrium of baboons (<i>Papio anubis</i>) with endometriosis

Joshi, Niraj; Su, Ren Wei; Chandramouli, Gadisetti V.R.; Khoo, Sok Kean; Jeong, Jaewook; Young, Steven L.; Lessey, Bruce A.; Fazleabas, A. T.

doi:10.1093/humrep/dev229

Cited by 34 publications

(49 citation statements)

References 87 publications

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“…According to the widely accepted theory of retrograde menstruation, the endometrium is the source of ectopic endometriotic foci. Previous studies showed that the eutopic endometrium contributed to the pathogenesis of endometriosis due to the increase of proliferation, migration and invasion of ectopic endometrium [29][30][31][32][33]. In this study, we did not detect a significant difference in patients' uric acid level in serum.…”

Section: Discussioncontrasting

confidence: 81%

Endometrium metabolomic profiling reveals potential biomarkers for diagnosis of endometriosis at minimal-mild stages

Liang

2018

Fertility and Sterility

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Background: The sensitivity and specificity of non-invasive diagnostic methods for endometriosis, especially at early stages, are not optimal. The clinical diagnostic indicator cancer antigen 125 (CA125) performs poorly in the diagnosis of minimal endometriosis, with a sensitivity of 24%. Therefore, it is urgent to explore novel diagnostic biomarkers. We evaluated the metabolomic profile variation of the eutopic endometrium between minimal-mild endometriosis patients and healthy women by ultra-high-performance liquid chromatography coupled with electrospray ionization high-resolution mass spectrometry (UHPLC-ESI-HRMS). Methods: Our study comprised 29 patients with laparoscopically confirmed endometriosis at stages I-II and 37 infertile women who underwent diagnostic laparoscopy combined with hysteroscopy from January 2014 to January 2015. Eutopic endometrium samples were collected by pipelle endometrial biopsy. The metabolites were quantified by UHPLC-ESI-HRMS. The best combination of biomarkers was then selected by performing step-wise logistic regression analysis with backward elimination. Results: Twelve metabolites were identified as endometriosis-associated biomarkers. The eutopic endometrium metabolomic profile of the endometriosis patients was characterized by a significant increase in the concentration of hypoxanthine, L-arginine, L-tyrosine, leucine, lysine, inosine, omega-3 arachidonic acid, guanosine, xanthosine, lysophosphatidylethanolamine and asparagine. In contrast, the concentration of uric acid was decreased. Metabolites were filtered by step-wise logistic regression with backward elimination, and a model containing uric acid, hypoxanthine, and lysophosphatidylethanolamine was constructed. Receiver-operating characteristic (ROC) analysis confirmed the prognostic value of these parameters for the diagnosis of minimal/mild endometriosis with a sensitivity of 66.7% and a specificity of 90.0%. Conclusions: Metabolomics analysis of the eutopic endometrium in endometriosis was effectively characterized by UHPLC-ESI-HRMS-based metabolomics. Our study supports the importance of purine and amino acid metabolites in the pathophysiology of endometriosis and provides potential biomarkers for semi-invasive diagnosis of early-stage endometriosis.

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Section: Discussioncontrasting

confidence: 81%

Endometrium metabolomic profiling reveals potential biomarkers for diagnosis of endometriosis at minimal-mild stages

Liang

2018

Fertility and Sterility

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“…Lastly, overexpression of miR-451 in an immortalized human endometriotic epithelial cell line (12Z) led to the decreased expression of its target YWHAZ which was correlated with decreased cell proliferation. Collectively, both our study 46 and that of Joshi and colleagues 47 demonstrated that miR-451 is capable of modulating cell proliferation and this is associated with alterations in the expression of targets MIF and YWHAZ.…”

Section: Putative Targets Of and Functions For Specific Mirnas In Esupporting

confidence: 63%

“…An additional study by Joshi and colleagues 47 identified miR-451 mis-expression in women with endometriosis as well as in baboons with induced disease. Induction of endometriosis in baboons results in altered expression of several miRNAs including miR-451 .…”

Section: Putative Targets Of and Functions For Specific Mirnas In Ementioning

confidence: 97%

MicroRNAs and Endometriosis: Distinguishing Drivers from Passengers in Disease Pathogenesis

Nothnick

2017

Semin Reprod Med

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Endometriosis is a disease common in women of reproductive age, characterized by pelvic pain and infertility. Despite its prevalence, the factors and mechanisms which contribute to the development and survival of ectopic lesions remain uncertain. MicroRNAs (miRNAs) are small RNA molecules that regulate posttranscriptional gene regulation which have been proposed to contribute to the pathogenesis of many diseases including that of endometriosis. This review summarizes the results of initial studies describing differentially expressed miRNAs between endometriotic lesion tissue and eutopic endometrium. Focus then moves toward discussion of studies on examining function of differentially expressed miRNAs to determine if they play a permissive role (driver of the disease) in events conducive to endometriosis progression/survival. Included in this discussion are the potential targets of these miRNAs and how their mis-expression may contribute to the disease. Limitations and challenges faced in studying miRNAs and endometriosis pathogenesis and recommendations to overcome these hurdles are presented at the end.

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“…Eutopic endometrium contributed to pathogenesis of endometriosis due to the increase of proliferation, migration, and invasion of ectopic endometrium (Joshi et al, 2015 ; Laudanski et al, 2015 ). We detected PS (20:3/23:1) decreased significantly in endometriosis group in this study.…”

Section: Discussionmentioning

confidence: 99%

Discovery of Phosphatidic Acid, Phosphatidylcholine, and Phosphatidylserine as Biomarkers for Early Diagnosis of Endometriosis

Gao

Guan

et al. 2018

Front. Physiol.

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The sensitivity and specificity of clinical diagnostic indicators and non-invasive diagnostic methods for endometriosis at early stage is not optimal. Previous studies demonstrated that abnormal lipid metabolism was involved in the pathological development of endometriosis. Our cross-sectional study included 21 patients with laparoscopically confirmed endometriosis at stage I–II and 20 infertile women who underwent diagnostic laparoscopy combined with hysteroscopy from January 2014 to January 2015. Eutopic endometrium was collected by pipelle endometrial biopsy. Lipid metabolites were quantified by ultra-high performance liquid chromatography coupled with electrospray ionization high-resolution mass spectrometry (UHPLC-ESI-HRMS). Lipid profiles of endometriosis patients at early stage (I–II) was characterized by a decreased concentration of phosphatidylcholine (18:1/22:6), (20:1/14:1), (20:3/20:4), and phosphatidylserine (20:3/23:1) and an increased concentration of phosphatidic acid (25:5/22:6) compared with control. The synthesized predicting strategy with 5 biomarkers has a specificity of 75.0% and a sensitivity of 90.5%. Lipid profile of eutopic endometrium in endometriosis was effectively characterized by UHPLC-ESI-HRMS-based metabolomics. Our study demonstrated the alteration of phosphatidic acid, phosphatidylcholine, phosphatidylserine metabolites in endometriosis and provided potential biomarkers for semi-invasive diagnose of endometriosis at early stage.

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Altered expression of microRNA-451 in eutopic endometrium of baboons (Papio anubis) with endometriosis

Cited by 34 publications

References 87 publications

Endometrium metabolomic profiling reveals potential biomarkers for diagnosis of endometriosis at minimal-mild stages

Endometrium metabolomic profiling reveals potential biomarkers for diagnosis of endometriosis at minimal-mild stages

MicroRNAs and Endometriosis: Distinguishing Drivers from Passengers in Disease Pathogenesis

Discovery of Phosphatidic Acid, Phosphatidylcholine, and Phosphatidylserine as Biomarkers for Early Diagnosis of Endometriosis

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