Altered expression of phosphatase of regenerating liver gene family in non-small cell lung cancer

Lee, Seung‐Hyo

doi:10.3892/or.2011.1495

Cited by 2 publications

(2 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…26 Many studies have reported that PTP4A2 expression is upregulated in breast 27 and lung cancer. 28 SNH significantly decreased PTP4A2 expression in H1299 cells (Fig. 5a, b, Table S1), which agreed with the observations of SNHinduced G0/G1 arrest in H1299 cells (Fig.…”

Section: Snh Reduces the Expression Of Cell Cycle-related Proteinssupporting

confidence: 90%

Quantitative Proteomics Reveals the Antitumor Effects of Sodium New Houttuyfonate on Non-small Cell Lung Cancer

Yang¹,

Wang²,

Le³

et al. 2021

Journal of Exploratory Research in Pharmacology

View full text Add to dashboard Cite

Background and objectives: Houttuynia cordata Thunb, which is a traditional Chinese herbal medicine, is commonly used as an anti-inflammatory, antiviral, and antibacterial agent in China. Emerging evidence shows that extracts of H. cordata Thunb have anticancer activity in human colorectal, leukemic, lung, and liver cancer cells; however, the specific active ingredients or compounds that responsible for these anticancer activities and their mechanism of action remain unknown. Sodium new houttuyfonate (SNH) is an additional product of the active ingredient houttuynin from H. cordata Thunb, which possesses anticancer activity; however, the molecular mechanisms that underlie its action have not been clarified. This study aims to explore the antitumor effect and related molecular mechanism of SNH on human non-small cell lung cancer (NSCLC). Methods:The cytotoxicity of SNH against human lung cancer cells H1299 was investigated using WST-1 and apoptotic assays, and its antitumor molecular mechanism was explored using quantitative proteomics combined with various cellular and biochemical assays. Results:The results showed that SNH reduced the viability and enhanced the apoptosis of H1299 cells in a dosedependent manner. Quantitative proteomics and ingenuity pathway analysis revealed that SNH downregulated the expression of cell cycle-related proteins, which included cyclin-dependent kinase 1 (CDK1), protein tyrosine phosphatase type IVA 2 (PTP4A2), and cyclin-dependent kinase 6 (CDK6), and upregulated the expression of Nrf2 (nuclear factor erythroid 2-related factor 2)-mediated oxidative stress response-related proteins in H1299 cells.Conclusions: SNH-induced G0/G1 arrest and apoptosis in H1299 cells by the inhibition of cell cycle-related proteins that included CDK1, PTP4A2, CDK6, and activated the expression of Nrf2-mediated oxidative stress response-related proteins. These findings might provide new molecular mechanisms that underlie the antitumor activity of SNH against NSCLC and could implicate SNH as a novel therapeutic drug for NSCLC in the future.

show abstract

Section: Snh Reduces the Expression Of Cell Cycle-related Proteinssupporting

confidence: 90%

Quantitative Proteomics Reveals the Antitumor Effects of Sodium New Houttuyfonate on Non-small Cell Lung Cancer

Yang¹,

Wang²,

Le³

et al. 2021

Journal of Exploratory Research in Pharmacology

View full text Add to dashboard Cite

show abstract

“…Nevertheless, there are few conflicting studies that have described the correlation between PRL-3 expression and lung cancer progression. Two reports showed no PRL-3 increase in lung cancer tissues compared with normal ones [15, 28], whereas another showed that PRL-3 is overexpressed in NSCLC and correlated with clinical stage [29]. However, our data confirmed the role of PRL-3 in lung cancer and indicated that PRL-3 expression negatively correlates with lung cancer cell motility and growth (Figures 3 and 4).…”

Section: Discussionsupporting

confidence: 49%

Phosphatase of regenerating liver-3 inhibits invasiveness and proliferation in non-small cell lung cancer by regulating the epithelial-mesenchymal transition

Lin

Lee

et al. 2016

Oncotarget

View full text Add to dashboard Cite

Phosphatase of regenerating liver-3 (PRL-3) has been reported to be associated with colon and gastric cancer metastasis. However, the role and function of PRL-3 in human non-small cell lung cancer cells is unknown. Our studies showed that the expression of PRL-3mRNA and protein are higher in less invasive human lung adenocarcinoma cells than in highly invasive cell lines. Ectopic expression of PRL-3 reduced cell capacity for anchorage-dependent growth, anchorage-independent growth, migration, and invasion in vitro, as well as tumorigenesis in vivo. Conversely, catalytic (C104S) and prenylation-site (C170S) mutants enhanced cell invasion. Microarray profiling of PRL-3 transfectants revealed the pathways potentially involving PRL-3, including the epithelial-mesenchymal transition (EMT), extracellular matrix remodeling, and the WNT signaling pathway. Furthermore, we demonstrated that increased PRL-3 reduced Slug and enhanced E-cadherin gene expression through the AKT/GSK3β/β-catenin pathway. In conclusion, our data suggest that PRL-3 might play a tumor suppressor role in lung cancer, distinct from other cancers, by inhibiting EMT-related pathways.

show abstract

Altered expression of phosphatase of regenerating liver gene family in non-small cell lung cancer

Cited by 2 publications

References 23 publications

Quantitative Proteomics Reveals the Antitumor Effects of Sodium New Houttuyfonate on Non-small Cell Lung Cancer

Quantitative Proteomics Reveals the Antitumor Effects of Sodium New Houttuyfonate on Non-small Cell Lung Cancer

Phosphatase of regenerating liver-3 inhibits invasiveness and proliferation in non-small cell lung cancer by regulating the epithelial-mesenchymal transition

Contact Info

Product

Resources

About