Altered Fatty Acid Profile in the Liver and Serum of Stroke-Prone Spontaneously Hypertensive Rats: Reduced Proportion of cis-Vaccenic Acid

Tanaka, Sayoko; Kojiguchi, Chiho; Yamazaki, Tohru; Mitsumoto, Atsushi; Kobayashi, Daisuke; Kudo, Naomi; Kawashima, Yasunaru

doi:10.5650/jos.62.933

Cited by 7 publications

(6 citation statements)

References 48 publications

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“…Similarly, several other studies demonstrate that 38:4 species are major phospholipids in the rat liver (Burdge et al, 1994; Wood and Harlow, 1969b). Moreover, the fatty acid composition of TAGs in our study was similar to earlier studies (Fukuchi et al, 2004; Tanaka et al, 2013; Wood and Harlow, 1969b), with linoleic acid, oleic acid and palmitic acid being major FA TAGs in the whole rat liver. These three fatty acids are also major FA TAGs in the mouse liver (Nault et al, 2017) and are abundant constituents of rodent chow (Angrish et al, 2012).…”

Section: Discussionsupporting

confidence: 91%

Identification of lipidomic markers of chronic 3,3′,4,4′,5-pentachlorobiphenyl (PCB 126) exposure in the male rat liver

Kania-Korwel

Wang

et al. 2017

Toxicology

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Exposure to PCB 126, an environmentally relevant aryl hydrocarbon receptor agonist, is an environmental factor causing hepatic steatosis in rodent models; however, the lipidome of PCB 126-exposed rats has not been investigated in-depth. The objective of the present study was therefore to characterize dose-dependent changes in the lipid profile in the liver of male Sprague-Dawley rats exposed to PCB 126. Rats were exposed for three month to intraperitoneal injections of 0.01, 0.05 and 0.2 μmol/kg bw PCB 126 in corn oil. Control animals were exposed in parallel and received corn oil alone. Lipids were extracted from whole liver homogenate and levels of polar lipids and fatty acids incorporated into triglycerides (FATAGs) were determined with tandem mass spectrometry using electrospray ionization. PCB 126 exposure increased the hepatic content of polar lipids and FATAGs. Protein adjusted levels of several polar lipid classes, in particular phosphatidylserine levels, decreased, whereas FATAGs levels typically increased with increasing PCB 126 dose. Sensitive, dose-dependent endpoints of PCB 126 exposure included an increase in levels of adrenic acid incorporated into triglycerides and changes in levels of certain ether-linked phospholipid and 1-alkyl/1-alkenyldiacylglycerol species, as determined using partial least square discriminant analysis (PLS-DA) and ANOVA. These changes in the composition of polar lipids and fatty acid in the liver of PCB 126 exposed rats identified several novel markers of PCB 126-mediated fatty liver disease that need to be validated in further studies.

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Section: Discussionsupporting

confidence: 91%

Identification of lipidomic markers of chronic 3,3′,4,4′,5-pentachlorobiphenyl (PCB 126) exposure in the male rat liver

Kania-Korwel

Wang

et al. 2017

Toxicology

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“…Recent studies have shown that this FA could represent a potent marker of various diseases such as chronic kidney disease [45], hypertension [46] or heart failure [47]. Tripathy et al also recently demonstrated that 18:1n-7 was inversely associated with hepatic enzymes involved in gluconeogenesis, glucose intolerance, and hyperglycemia [48].…”

Section: Discussionmentioning

confidence: 99%

Impact of a Standard Rodent Chow Diet on Tissue n‐6 Fatty Acids, Δ9‐Desaturation Index, and Plasmalogen Mass in Rats Fed for One Year

Pédrono

Boulier-Monthéan

Catheline

et al. 2015

Lipids

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Although many studies focus on senescence mechanisms, few habitually consider age as a biological parameter. Considering the effect of interactions between food and age on metabolism, here we depict the lipid framework of 12 tissues isolated from Sprague-Dawley rats fed standard rodent chow over 1 year, an age below which animals are commonly studied. The aim is to define relevant markers of lipid metabolism influenced by age in performing a fatty acid (FA) and dimethylacetal profile from total lipids. First, our results confirm impregnation of adipose and muscular tissues with medium-chain FA derived from maternal milk during early infancy. Secondly, when animals were switched to standard croquettes, tissues were remarkably enriched in n-6 FA and especially 18:2n-6. This impregnation over time was coupled with a decrease of the desaturation index and correlated with lower activities of hepatic Δ5- and Δ6-desaturases. In parallel, we emphasize the singular status of testis, where 22:5n-6, 24:4n-6, and 24:5n-6 were exceptionally accumulated with growth. Thirdly, 18:1n-7, usually found as a discrete FA, greatly accrued over the course of time, mostly in liver and coupled with Δ9-desaturase expression. Fourthly, skeletal muscle was characterized by a surprising enrichment of 22:6n-3 in adults, which tended to decline in older rats. Finally, plasmalogen-derived dimethylacetals were specifically abundant in brain, erythrocytes, lung, and heart. Most notably, a shift in the fatty aldehyde moiety was observed, especially in brain and erythrocytes, implying that red blood cell analysis could be a good indicator of brain plasmalogens.

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“…An interesting fi nding indicates an unfavorable association of reduced levels of VA in plasma and liver of hypertensive rats with the incidence of stroke-like metabolic features [27]. The inverse association of cis-VA has been demonstrated in human studies investigating the correlation of its content in red blood cells and plasma with the incidence of myocardial infarction and heart failure with antecedent coronary heart disease, respectively [28,29].…”

Section: Discussionmentioning

confidence: 93%

Effects of Aronia melanocarpa juice on plasma and liver phospholipid fatty acid composition in Wistar rats

et al. 2017

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A nutritional placebo-controlled study was performed in Wistar rats in order to investigate the effects of 5-weeks aronia juice consumption towards fatty acid (FA) composition of phospholipids in the plasma and liver, as well as plasma glucose (Glu) and cholesterol levels. The animals were divided into 3 groups of 8 animals each, and randomized to receive either the full polyphenol dose of Aronia melanocarpa juice (AMJ), 4 times less polyphenol dose (¼-AMJ) or polyphenol-lacking placebo beverage (PLB). Each group of 8 male adult Wistar rats received the liquid ad libitum. AMJ decreased the levels of low-density lipoprotein (LDL) (P < 0.05) vs. PLB. AMJ increased dihomo-γ-linoleic acid (DGLA, 20:3n-6) (P < 0.05) and decreased arachidonic acid content (AA, 20:4n-6) (P < 0.05) vs. PLB in liver phospholipids. AMJ signifi cantly increased monounsaturated fatty acids (MUFA) levels both in the liver (P < 0.05) and plasma (P < 0.05). Both aronia juice doses elevated the levels of benefi cial n-3 polyunsaturated fatty acids (PUFA) in the plasma and liver. There was a dose-dependent, signifi cant increase (P < 0.001) in cis-vaccenic acid (VA, in phospholipids in the plasma and liver. Our results indicate favorable effects of aronia juice intake on lipid parameters in Wistar rats. These fi ndings suggest the potential of aronia dietary intake in cardiometabolic diseases primary prevention strategies in the human population.

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Altered Fatty Acid Profile in the Liver and Serum of Stroke-Prone Spontaneously Hypertensive Rats: Reduced Proportion of cis-Vaccenic Acid

Cited by 7 publications

References 48 publications

Identification of lipidomic markers of chronic 3,3′,4,4′,5-pentachlorobiphenyl (PCB 126) exposure in the male rat liver

Identification of lipidomic markers of chronic 3,3′,4,4′,5-pentachlorobiphenyl (PCB 126) exposure in the male rat liver

Impact of a Standard Rodent Chow Diet on Tissue n‐6 Fatty Acids, Δ9‐Desaturation Index, and Plasmalogen Mass in Rats Fed for One Year

Effects of Aronia melanocarpa juice on plasma and liver phospholipid fatty acid composition in Wistar rats

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