Altered intracellular purine nucleotides in gamma‐irradiated red blood cell concentrates

Leitner, Gerda; Neuhäuser, Markus; Weigel, Günter; Kurze, S.; Fischer, Michael B.; Höcker, P

doi:10.1046/j.1423-0410.2001.00082.x

Cited by 33 publications

(39 citation statements)

References 21 publications

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“…The decreased concentration of extracellular Na + can be associated with water influx to the cytosol. This process and the absence of a selective mechanism of functionality, as performed in the spleen, could explain the increase of MCV in vitro with consecutive morphological RBC changes [5,14,15,16,17]. …”

Section: Discussionmentioning

confidence: 99%

Biochemical and Cellular Changes in Leukocyte-Depleted Red Blood Cells Stored for Transfusion

Nogueira¹,

Rocha²,

Abreu³

et al. 2014

Transfus Med Hemother

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Background: To evaluate biochemical and cellular changes associated with the storage of leukocyte-depleted red blood cells (RBCs). Methods: We investigated 10 leukocyte-depleted RBC units, randomly chosen from volunteer donors. Every week an aliquot was collected for laboratorial evaluation, which included complete cell blood count, glucose-6-phosphate dehydrogenase (G6PD) activity, extracellular sodium, potassium and pH, membrane-bound hemoglobin (MBH), band 3 profile, and quantification of RBC membrane proteins composition. Results: We observed an increase in mean cell volume (from 91.86 ± 4.65 fl to 98.10 ± 5.80 fl, day 0 vs. day 21; p < 0.05), red cell distribution width, percentage of macrocytic RBCs, reticulocyte hemoglobin content and a decreased percentage of microcytic RBCs, mean cell volume concentration and G6PD activity. The extracellular concentration of sodium decreased, and that of potassium increased significantly over time. RBC membrane composition revealed an increase in spectrin/ankyrin ratio after 21 days (from 4.84 ± 0.99 to 5.27 ± 0.94, day 0 vs. day 21; p < 0.05). At day 35, a decrease in ankyrin (from 6.44 ± 1.70% to 5.49 ± 1.96%, day 0 vs. day 35; p < 0.05), in protein 4.1/band 3, protein 4.2/band 3, and ankyrin/band 3 ratios and in band 5 was observed. Conclusions: Our data show that leukocyte-depleted RBCs present changes in the RBC morphology, membrane protein composition, enzymatic activity, and extracellular electrolyte concentration and pH.

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Section: Discussionmentioning

confidence: 99%

Biochemical and Cellular Changes in Leukocyte-Depleted Red Blood Cells Stored for Transfusion

Nogueira¹,

Rocha²,

Abreu³

et al. 2014

Transfus Med Hemother

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“…The leakage of LDH to RBC medium can occur through direct membrane disruption (RBC lysis with concomitant release of LDH and Hb). An excessive LDH release at the end of the storage period could also be associated with depletion of intracellular energy-rich nucleosides (ATP, ADP, ITP (inositol triphosphate)) [37]. The enhanced energy loss that arises at the 2-to 4-week storage can lead to insufficient phosphorylation of membrane proteins and a mismatch of membrane skeleton assembly resulting in a serious reduction of the membrane's mechanical stability and in an increased permeability.…”

Section: Discussionmentioning

confidence: 99%

Vitamin E Analogue Protects Red Blood Cells against Storage-Induced Oxidative Damage

Antosik

Czubak

Cichoń

et al. 2018

Transfus Med Hemother

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Background: To investigate i) the effects of Trolox® or mannitol, which represent two different classes of antioxidants, on oxidative changes generated in manually isolated red blood cells (RBCs) from citrate-phosphate-dextrose (CPD) preserved whole blood, followed by up to 20 days refrigerated storage, and ii) whether Trolox supplemented to the blood bank-manufactured saline-adenine-glucose-mannitol (SAGM) preserved RBC units would offer better storage conditions compared with SAGM alone. Methods: The percentage of hemolysis and extracellular activity of lactate dehydrogenase (LDH) was measured to assess RBC membrane integrity. Lipid peroxidation, reduced glutathione (GSH) levels and total antioxidant capacity (TAC) were quantified by thiobarbituric acid-reactive substances (TBARS), Ellman's reagent and 2, 2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate) (ABTS.+) based assay, respectively. Results: Trolox was little more effective than mannitol in protecting against progressive RBC hemolysis. Trolox (0.125-3.125 mmol/l) inhibited storage-induced leakage of LDH, lipid peroxidation, and to a lesser extent GSH depletion. Mannitol at these concentrations neither inhibited TBARS formation nor prevented GSH depletion. RBC units stored in SAGM-Trolox had significantly lower hemolysis, LDH leakage, and lipid peroxidation level compared to RBCs stored in SAGM. Conclusion: There is evidence of the beneficial effects of supplementing RBC-additive solutions with membrane-interacting antioxidants such as vitamin E analogues.

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“…Before each measurement, the instrument was calibrated to a pH-value between 4AE0 and 7AE4 using standard controls. Intracellular nucleotides (ATP, ADP and AMP) were determined by highperformance liquid chromatography (HPLC) as described previously [13]. For sterility testing in all fractions, we used the BacT ⁄ ALERT Ò FA system for aerobic and anaerobic culture (Biomerieux, INC., Durham, Brasil S.A.) Statistics Statistical analyses were performed by Friedman's analysis of variance (ANOVA) test.…”

Section: Laboratory Analysesmentioning

confidence: 99%

“…Special attention was paid to intracellular purine content by measuring ATP, ADP, AMP, as a surrogate marker for RBC function [11][12][13].…”

Section: Introductionmentioning

confidence: 99%

“…When paediatric BM donors were investigated and BM-RBCs were separated by density gradient centrifugation using Ficoll-Hypaque, similar results were In a prospective analysis, we assessed the extent of cell lesions occurring during manipulation by measuring the release of lactate dehydrogenase (LDH), free haemoglobin (fHb), potassium (K+) and lactate at each manipulation step. Special attention was paid to intracellular purine content by measuring ATP, ADP, AMP, as a surrogate marker for RBC function [11][12][13]. …”

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confidence: 99%

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Red blood units collected from bone marrow harvests after mononuclear cell selection qualify for autologous use

et al. 2010

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Background After large volume bone marrow (BM) harvest, donors and patients can develop severe anaemia, because collected BM can contain up to 20% of their red cell mass. In a prospective analysis, we investigated the feasibility to recover red blood cells (RBCs) from the harvested BM and investigated whether these RBC units meet the quality requirements of the European Council.Patients and Methods From 19 patients (median age 51 yrs, range 31-77) with acute myocardial infarction, who participated in the MYSTAR study, a median volume of 1299 ml (range, 700-1870 ml) BM was collected. During BM processing, mononuclear cells (MNC) were separated using the Cobe SpectraÔ apheresis system and the residual RBCs were collected in a separate bag. The quality of the collected RBCs was assessed by measuring LDH, free haemoglobin, potassium and lactate. Haemolysis was calculated and the intracellular concentration of ATP, ADP, AMP was determined by HPLC.Results RBC units recovered from BM after MNC separation had a mean volume of 312 ± 95 ml with a haematocrit of 47 ± 8AE9%, a haemoglobin content of 51 ± 15 g per unit, a haemolysis of 0AE15 ± 0AE005%, a pH of 6AE8 ± 0AE007 and an intracellular ATP concentration of 135 pmol ⁄ 10 6 RBC ± 41, which is comparable with freshly collected packed red blood cells (PRBCs).Conclusion RBCs, collected from bone marrow harvests, can be used for autologous blood support to minimize allogeneic blood transfusions in donors and patients after large volume BM donation.Key words: blood sparing measurements, bone marrow harvest, bone marrow processing, salvage of red blood cells. IntroductionLarge volume bone marrow (BM) harvest is an alternative to G-CSF-induced peripheral blood stem cell collection in patients or donors who are poor mobilizers, in the paediatric setting or in the regenerative medicine, when patients with myocardial infarction are intended to receive stem cells intracardially [1][2][3][4]. Donors and patients are at risk to develop a severe anaemia because collected BM can contain up to 20% of their red cell mass. To reduce the risk of anaemia after BM donation, at least one unit of autologous blood should be collected prior to the harvest procedure [5]. Alternatively, certain clinicians place the donors on supplemental iron plus rhEPO, transfuse their donors with allogeneic RBC units or tolerate anaemia when not severe [6]. Previous studies have shown that a recovery of approximately 90% of the harvested BM-RBCs decreased the homologous transfusion requirement of healthy BM donors significantly [7]. When paediatric BM donors were investigated and BM-RBCs were separated by density gradient centrifugation using Ficoll-Hypaque, similar results were In a prospective analysis, we assessed the extent of cell lesions occurring during manipulation by measuring the release of lactate dehydrogenase (LDH), free haemoglobin (fHb), potassium (K+) and lactate at each manipulation step. Special attention was paid to intracellular purine content by measuring ATP, ADP, AMP, as a surrogate mar...

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Altered intracellular purine nucleotides in gamma‐irradiated red blood cell concentrates

Abstract: Gamma irradiation of SAGM-preserved RCCs leads to serious deterioration of the purine nucleotide metabolism of erythrocytes during storage, which can reduce the in vivo recovery of the transfused red cells.

Cited by 33 publications

References 21 publications

Biochemical and Cellular Changes in Leukocyte-Depleted Red Blood Cells Stored for Transfusion

Biochemical and Cellular Changes in Leukocyte-Depleted Red Blood Cells Stored for Transfusion

Vitamin E Analogue Protects Red Blood Cells against Storage-Induced Oxidative Damage

Red blood units collected from bone marrow harvests after mononuclear cell selection qualify for autologous use

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