Altered neurogenic and mechanical responses to acetylcholine, ATP and substance P in detrusor from rat with outlet obstruction

Pinna, Christian; Sanvito, Paola; Puglisi, L.

doi:10.1016/j.lfs.2006.03.035

Cited by 14 publications

(13 citation statements)

References 23 publications

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“…Interestingly, rats void with intermittent pulsatile activity of the rhabdosphincter, which is more prominent in male animals [50]. As this pulsatile activity is intermittently obstructive, the finding of a greater purinergic contractile contribution in males is consistent with findings of altered cholinergic/purinergic balance in chronic rat bladder obstruction models [51][52][53].…”

Section: P2x1 and Detrusor Contractionsupporting

confidence: 66%

“…Several studies have demonstrated impaired cholinergic responses and unchanged purinergic responsiveness in chronic outlet obstruction rat models, suggesting a relatively increased purinergic contribution to detrusor contraction [52][53][54]. In contrast, a decrease in purinergic agonist-induced contractile force was observed in hypertrophied rat bladder strips following outlet obstruction [55].…”

Section: P2x1 and Detrusor Contractionmentioning

confidence: 99%

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Purinoceptors and Bladder Dysfunction

Smith

2011

Curr Bladder Dysfunct Rep

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Purinergic signaling is increasingly appreciated as an important contributor to the normal bladder functions of urine storage and voiding. The translation of disease and injury to abnormal sensory and motor function, resulting in lower urinary tract symptoms, is in part mediated by the interaction of adenosine 5′-triphosphate and related purines with specific purinergic receptors (purinoceptors). Identification and clarification of purinoceptor-based processes are important research goals aimed at novel therapeutic targets for functional disorders inadequately served by standard therapies. This review presents an update on recent developments in the understanding of the purinoceptors and bladder dysfunction.

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Section: P2x1 and Detrusor Contractionsupporting

confidence: 66%

Section: P2x1 and Detrusor Contractionmentioning

confidence: 99%

Purinoceptors and Bladder Dysfunction

Smith

2011

Curr Bladder Dysfunct Rep

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“…Sensory neurons innervating the distal colon have previously been shown to respond to several of the algesic compounds examined in this study, including ATP (Wynn et al 2004), ACh (Darko et al 1997), and capsaicin (Su et al 1999). Although there have been numerous algesic response studies focused on other regions of the rat lower urinary tract (e.g., detrusor and urothelium of the bladder, proximal urethra) showing the effects of ATP (e.g., Brown et al 1979;Dang et al 2005;Pinna et al 2006;Yu and de Groat 2008), ACh (e.g., Hashitani and Suzuki 1996;Kanai et al 2007;Pinna et al 2006), protons (Dang et al 2005Sadananda et al 2009), andcapsaicin (e.g., Dang et al 2005;Masuda et al 2006;Shea et al 2000), few have examined algesic responses within the distal urethra and glans penis. However, in neurons innervating the colon and penis of the rat, some of the channels that mediate responses to algesic compounds have been identified immunohistochemically, including those for ATP-responsive purinergic P2X receptors (colon: Van Crombruggen et al 2007;Wynn et al 2003;and penis: Lee et al 2000), and proton-and capsaicin-sensitive TRPV1 (colon: Christianson et al 2006;Rau et al 2007;and penis: Stein et al 2004).…”

Section: Identification Of Cell Types Innervating the Colon And Penismentioning

confidence: 70%

Distinct subclassification of DRG neurons innervating the distal colon and glans penis/distal urethra based on the electrophysiological current signature

Rau

Petruska

Cooper

et al. 2014

Journal of Neurophysiology

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Rau KK, Petruska JC, Cooper BY, Johnson RD. Distinct subclassification of DRG neurons innervating the distal colon and glans penis/distal urethra based on the electrophysiological current signature. J Neurophysiol 112: 1392-1408, 2014. First published May 28, 2014 doi:10.1152/jn.00560.2013.-Spinal sensory neurons innervating visceral and mucocutaneous tissues have unique microanatomic distribution, peripheral modality, and physiological, pharmacological, and biophysical characteristics compared with those neurons that innervate muscle and cutaneous tissues. In previous patch-clamp electrophysiological studies, we have demonstrated that small-and medium-diameter dorsal root ganglion (DRG) neurons can be subclassified on the basis of their patterns of voltage-activated currents (VAC). These VAC-based subclasses were highly consistent in their action potential characteristics, responses to algesic compounds, immunocytochemical expression patterns, and responses to thermal stimuli. For this study, we examined the VAC of neurons retrogradely traced from the distal colon and the glans penis/distal urethra in the adult male rat. The afferent population from the distal colon contained at least two previously characterized cell types observed in somatic tissues (types 5 and 8), as well as four novel cell types (types 15, 16, 17, and 18). In the glans penis/distal urethra, two previously described cell types (types 6 and 8) and three novel cell types (types 7, 14, and 15) were identified. Other characteristics, including action potential profiles, responses to algesic compounds (acetylcholine, capsaicin, ATP, and pH 5.0 solution), and neurochemistry (expression of substance P, CGRP, neurofilament, TRPV1, TRPV2, and isolectin B4 binding) were consistent for each VAC-defined subgroup. With identification of distinct DRG cell types that innervate the distal colon and glans penis/distal urethra, future in vitro studies related to the gastrointestinal and urogenital sensory function in normal as well as abnormal/pathological conditions may be benefitted. viscera; sensory neuron; nociception; dorsal root ganglion; classification THE DISTAL COLON AND GLANS PENIS/DISTAL URETHRA are innervated by a complex network of sensory neurons that encode and transmit information to the central nervous system regarding the state of the gastrointestinal and urogenital tracts, respectively. Although they share many aspects of cellular anatomy, physiology, and function similar to those of neurons found in the somatic regions of skin and muscle, the ultrastructure and environment of visceral (colon) and mucocutaneous (glans penis/distal urethra) tissues present unique stimuli and conditions to their embedded sensory terminals.Sensory neurons innervating these tissues conduct nociceptive and nonnociceptive information to the spinal cord, including responses to chemical and mechanical stimuli in both tissues and thermal stimuli in the glans penis. In both the colon and penis, sensory neurons integrate with various interneurons, pseudomotor neuro...

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“…Suramin, a P2 receptor antagonist, enhanced the inhibitory action of atropine in detrusor from rats with outlet obstruction [562]. The authors suggest that this might be of interest in the therapy of patients with bladder incontinence caused by detrusor overactivity, who fail to respond to even the maximum dosage of antimuscarinic drugs.…”

Section: Bladder Outflow Obstructionmentioning

confidence: 95%

Purinergic signalling in the urinary tract in health and disease

Burnstock

2013

Purinergic Signalling

143

149

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Purinergic signalling is involved in a number of physiological and pathophysiological activities in the lower urinary tract. In the bladder of laboratory animals there is parasympathetic excitatory cotransmission with the purinergic and cholinergic components being approximately equal, acting via P2X1 and muscarinic receptors, respectively. Purinergic mechanosensory transduction occurs where ATP, released from urothelial cells during distension of bladder and ureter, acts on P2X3 and P2X2/3 receptors on suburothelial sensory nerves to initiate the voiding reflex, via low threshold fibres, and nociception, via high threshold fibres. In human bladder t h e p u r i n e rg i c c o m p o n e n t o f p a r a s y m p a t h e t i c cotransmission is less than 3 %, but in pathological conditions, such as interstitial cystitis, obstructed and neuropathic bladder, the purinergic component is increased to 40 %. Other pathological conditions of the bladder have been shown to involve purinoceptor-mediated activities, including multiple sclerosis, ischaemia, diabetes, cancer and bacterial infections. In the ureter, P2X7 receptors have been implicated in inflammation and fibrosis. Purinergic therapeutic strategies are being explored that hopefully will be developed and bring benefit and relief to many patients with urinary tract disorders.

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Altered neurogenic and mechanical responses to acetylcholine, ATP and substance P in detrusor from rat with outlet obstruction

Cited by 14 publications

References 23 publications

Purinoceptors and Bladder Dysfunction

Purinoceptors and Bladder Dysfunction

Distinct subclassification of DRG neurons innervating the distal colon and glans penis/distal urethra based on the electrophysiological current signature

Purinergic signalling in the urinary tract in health and disease

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