Altered profiles of nuclear matrix proteins during the differentiation of human gastric mucous adenocarcinoma MGc80-3 cells

Zhao, Chen; Li, Qifu

doi:10.3748/wjg.v11.i30.4628

Cited by 12 publications

(8 citation statements)

References 15 publications

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“…The electrophoresis identified 41 NMPs that were differentially expressed during the differentiation process. Previous studies in other cell lines showed that the composition of the nuclear matrix varies during the different stages of cell differentiation [Zhao and Li, 2005]. Similarly, our own studies confirmed that the HMBAinduced differentiation of the human osteosarcoma cell line MG-63 was accompanied by changes of NMPs [Zhao et al, 2006a].…”

Section: Discussionsupporting

confidence: 77%

Differential expression of nuclear matrix proteins during the differentiation of human neuroblastoma SK‐N‐SH cells induced by retinoic acid

Liang¹,

Li²,

Zhang³

et al. 2009

J of Cellular Biochemistry

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To investigate the alteration of nuclear matrix proteins (NMPs) during the differentiation of neuroblastoma SK-N-SH cells induced by retinoic acid (RA), differentiation markers were detected by immunocytochemistry and NMPs were selectively extracted and subjected to two-dimensional gel electrophoresis analysis. Immunocytochemical observation demonstrated that the expression of neuronal markers was up-regulated in SK-N-SH cells following RA treatment. Meanwhile, 52 NMPs (41 of which were identified) changed significantly during SK-N-SH differentiation; four of these NMPs were further confirmed by immunoblotting. This study suggests that the differentiation of neuroblastoma cells was accompanied by the altered expression of neuronal markers and NMPs. The presence of some differentially expressed NMPs was related to the proliferation and differentiation of neuroblastomas. Our results may help to reveal the relationship between NMPs and neuroblastoma carcinogenesis and reversion, as well as elucidate the regulatory principals driving neural cell proliferation and differentiation.

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Section: Discussionsupporting

confidence: 77%

Differential expression of nuclear matrix proteins during the differentiation of human neuroblastoma SK‐N‐SH cells induced by retinoic acid

Liang¹,

Li²,

Zhang³

et al. 2009

J of Cellular Biochemistry

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“…In a previous study, it was found that the expression of PHB is down-regulated during induced differentiation of MG-63, MGc80-3, and SMMC-7721 cells, indicating that PHB may be a common target protein for inducers in the cells (Li et al 2008a, b;Shi et al 2009;Zhao and Li 2005). More importantly, PHB is closely related to oncogenes and tumor suppressor genes and regulates cell proliferation and differentiation.…”

Section: Discussionmentioning

confidence: 92%

Localization of Prohibitin in the Nuclear Matrix and Alteration of Its Expression During Differentiation of Human Neuroblastoma SK-N-SH Cells Induced by Retinoic Acid

Liang

Shi

et al. 2010

Cell Mol Neurobiol

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The nuclear matrix-intermediate filament system of human neuroblastoma SK-N-SH cells before and after retinoic acid (RA) treatment was selectively extracted and the distribution of prohibitin (PHB) in the nuclear matrix, as well as its colocalization with related genes, was observed. Results of two-dimensional gel electrophoresis (2-DE), mass spectrometry (MS) identification, and protein immunoblotting all confirm that PHB was present in the components of SK-N-SH nuclear matrix proteins and was down-regulated after RA treatment. Immunofluorescence microscopy observations show that PHB was localized in the nuclear matrix and its distribution was altered due to RA treatment. Laser confocal microscopy results reveal that PHB colocalized with the expression products of c-myc, c-fos, p53, and Rb, but the colocalization region was altered after RA treatment. Our results prove that PHB is a nuclear matrix protein and is localized in nuclear matrix fibers. The distribution of PHB in SK-N-SH cells and its colocalization with related proto-oncogenes and tumor suppressor genes suggest that PHB plays pivotal roles in the differentiation of SK-N-SH cells and deserves further study.

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“…So far, the mechanism of its subcellular localization, nuclear transportation and regulation of cell proliferation and differentiation are not well understood. Our previous studies revealed that PHB existed in nuclear matrix extractions of human adenocarcinoma MGc80-3 cells, and its expression level was altered during the differentiation induced by hexamethylene bisacetamide (HMBA) [5] . Furthermore, in the differentiation of human osteosarcoma MG-63 cells, we observed similar results [6] .…”

Section: Introductionmentioning

confidence: 99%

Positional and expressive alteration of prohibitin during the induced differentiation of human hepatocarcinoma SMMC-7721 cells

Xu¹,

Tang²,

Li³

et al. 2008

WJG

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products of oncogenes or tumor repression genes including c-fos , c-myc , p53 and Rb and its alteration of distributive area in the cells treated by HMBA. CONCLUSION: These data confirm that PHB is a nuclear matrix protein, which is located in the nuclear matrix, and the distribution and expression of PHB and its relation with associated genes may play significant roles during the differentiation of SMMC-7721 cells. INTRODUCTIONProhibitin (PHB) is a tumor suppressor protein that is expressed in a variety of cell lines. It is not only localized to the inner membrane of mitochondria, where it acts as a chaperone protein, but is also present in the nucleus where it negatively regulates transcription. PHB plays important roles in the regulation of cell growth, proliferation, differentiation, aging and apoptosis. It is also involved in the genesis of tumor and some degenerative diseases [1][2][3] . In cell differentiation, some studies have found that the expression level of PHB was very low in rapidly proliferating cells, whereas it was much higher in cells undergoing differentiation. This indicates that PHB may promote cell differentiation and suppress cell proliferation [4] . Heretofore, overexpression of PHB has been found in various tumor cells. This seems to be conflictive with its tumor suppressive function. So far, the mechanism of its subcellular localization, nuclear transportation and regulation of cell proliferation and differentiation are Abstract AIM: To explore the existence and distribution of prohibitin (PHB) in nuclear matrix and its co-localization with products of some related genes during the differentiation of human hepatocarcinoma SMMC-7721 cells. METHODS:The nuclear matrix of the SMMC-7721 cells cultured with or without 5 × 10 -3 mmol/L hexamethylene bisacetamide (HMBA) was selectively extracted.Western blot was used to analyze the expression of PHB in nuclear matrix; immunofluorescence microscope observation was used to analyze the distribution of PHB in cell. LCSM was used to observe the co-localization of PHB with products of oncogenes and tumor suppressor genes. RESULTS: Western blot analysis showed that PHB existed in the composition of nuclear matrix proteins a n d wa s d o w n -re g u l a t e d b y H M B A t re a t m e n t . Immunofluorescence observation revealed that PHB existed in the nuclear matrix, and its distribution regions and expression levels were altered after HMBA treatment. Laser scanning confocal microscopy revealed the co-localization between PHB and the

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Altered profiles of nuclear matrix proteins during the differentiation of human gastric mucous adenocarcinoma MGc80-3 cells

Cited by 12 publications

References 15 publications

Differential expression of nuclear matrix proteins during the differentiation of human neuroblastoma SK‐N‐SH cells induced by retinoic acid

Differential expression of nuclear matrix proteins during the differentiation of human neuroblastoma SK‐N‐SH cells induced by retinoic acid

Localization of Prohibitin in the Nuclear Matrix and Alteration of Its Expression During Differentiation of Human Neuroblastoma SK-N-SH Cells Induced by Retinoic Acid

Positional and expressive alteration of prohibitin during the induced differentiation of human hepatocarcinoma SMMC-7721 cells

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