Altered transport properties in Escherichia coli mutants selected for pH-conditional growth on 3-deoxy-2-oxo-D-gluconate.

Mandrand‐Berthelot, Marie‐Andrée; Lagarde, A.

doi:10.1016/s0021-9258(18)34202-9

Cited by 2 publications

(4 citation statements)

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“…Our cloning experiments indicate that, besides the kdgT gene we were interested in, it also clones the entire rhaDABC operon and so at least five genes of the chromosomal DNA. According to previously published data (18), rhaD cotrahsduces with the kdgT gene at a frequency of 45%, a result suggesting that the map distance between these two genes was not greater than 0.48 min (1). By cloning this segrnent we demonstrated that the five genes are actually confined to a 10-kilobase fragmnent of bacterial DNA corresponding to 0.22 min, assumhing that a 1-min length of DNA corresponds to 45 kilobases (1).…”

Section: Discussionsupporting

confidence: 67%

“…Complementation for the unselected marker was checked by replica plating; 93% of the colicin-resistant strains of CHR2 were able to grow on rhamnose as the sole source of carbon. Therefore ColEl-kdgT appears to bear the rhaD gene and consequently includes the whole rhaDABC operon, since the order of the genes in this region was shown to be rhaD-rhaA-rhaB-rhaC-kdgT-pJkA-glpK-metB (18,30). The presence of rhaD on the ColEl-kdgT plasmid was confirmed by the transformation of strain CRH2 with the hybrid DNA preparation.…”

Section: Methodsmentioning

confidence: 88%

“…Enzymes assays. For KDG uptake assays, the cells were grown to the early stationary phase, centrifuged, and sus- (18). KDG kinase and KDPG aldolase were assayed as reported elsewhere (27,28).…”

Section: Methodsmentioning

confidence: 99%

“…Previous studies have demonstrated that KDG and Dglucuronate are concentrated into the cells via a sugar-H+ symport mechanism driven by the total proton motive force (11,12). Recently mutants of E. coli exhibiting pH-conditional growth on KDG were isolated to better understand the role played by protons in carrier-mediated translocation of substrates (18). The responsible mutations (kdgT phs) were also mapped into kdgT.…”

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confidence: 99%

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Construction and expression of hybrid plasmids containing the structural gene of the Escherichia coli K-12 3-deoxy-2-oxo-D-gluconate transport system

Mandrand‐Berthelot¹,

Ritzenthaler²,

Mata-Gilsinger³

1984

J Bacteriol

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The kdgT gene of Escherichia coli, which encodes for the 3-deoxy-2-oxo-D-gluconate transport system, was isolated as a ColE1-kdgT hybrid plasmid from the Clarke and Carbon bank. A restriction and genetic map of the min 88 region of the chromosome was established; by subcloning the restriction fragments into the plasmid vector pBR322, the kdgT gene was localized on a 1.4-megadalton PstI DNA fragment, and the direction of transcription of the gene was determined by making use of an in vitro gene fusion between kdgT and lacZ genes. Amplification of the gene product of kdgT was up to 14-fold the level found in a haploid strain. When plasmids bearing kdgT were expressed in an in vivo maxicell system, a specific polypeptide of 28,000 daltons appeared that was found to be associated with the membrane fraction.

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Section: Discussionsupporting

confidence: 67%

Section: Methodsmentioning

confidence: 88%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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Construction and expression of hybrid plasmids containing the structural gene of the Escherichia coli K-12 3-deoxy-2-oxo-D-gluconate transport system

Mandrand‐Berthelot¹,

Ritzenthaler²,

Mata-Gilsinger³

1984

J Bacteriol

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Catabolism of Hexuronides, Hexuronates, Aldonates, and Aldarates

Mandrand‐Berthelot

Condemine

Hugouvieux‐Cotte‐Pattat

2004

EcoSal Plus

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Following elucidation of the regulation of the lactose operon in Escherichia coli, studies on the metabolism of many sugars were initiated in the early 1960s. The catabolic pathways of D-gluconate and of the two hexuronates, D-glucuronate and D-galacturonate, were investigated. The post genomic era has renewed interest in the study of these sugar acids and allowed the complete characterization of the D-gluconate pathway and the discovery of the catabolic pathways for L-idonate, D-glucarate, galactarate, and ketogluconates. Among the various sugar acids that are utilized as sole carbon and energy sources to support growth of E. coli, galacturonate, glucuronate, and gluconate were shown to play an important role in the colonization of the mammalian large intestine. In the case of sugar acid degradation, the regulators often mediate negative control and are inactivated by interaction with a specific inducer, which is either the substrate or an intermediate of the catabolism. These regulators coordinate the synthesis of all the proteins involved in the same pathway and, in some cases, exert crosspathway control between related catabolic pathways. This is particularly well illustrated in the case of hexuronide and hexuronate catabolism. The structural genes encoding the different steps of hexuronate catabolism were identified by analysis of numerous mutants affected for growth with galacturonate or glucuronate. E. coli is able to use the diacid sugars D-glucarate and galactarate (an achiral compound) as sole carbon source for growth. Pyruvate and 2-phosphoglycerate are the final products of the D-glucarate/galactarate catabolism.

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Altered transport properties in Escherichia coli mutants selected for pH-conditional growth on 3-deoxy-2-oxo-D-gluconate.

Cited by 2 publications

References 51 publications

Construction and expression of hybrid plasmids containing the structural gene of the Escherichia coli K-12 3-deoxy-2-oxo-D-gluconate transport system

Construction and expression of hybrid plasmids containing the structural gene of the Escherichia coli K-12 3-deoxy-2-oxo-D-gluconate transport system

Catabolism of Hexuronides, Hexuronates, Aldonates, and Aldarates

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