Altered Wound Arginine Metabolism by Corticosterone and Retinoic Acid

Ulland, Anders E.; Shearer, Jeffry D.; Coulter, Cynthia; Caldwell, Michael D.

doi:10.1006/jsre.1997.5099

Cited by 33 publications

(21 citation statements)

References 20 publications

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“…16,24,25,29 NOx determinations have been proven to be highly sensitive in documenting declines in NO production associated with impaired wound healing and enhanced NO delivery associated with improved chronic wound recovery. Impaired wound healing caused by diabetes, 24 proteincalorie malnutrition, 30 cutaneous irradiation, 31 steroid therapy, 32 and metabolic inhibition of NO synthesis 33 was associated with reductions in wound fluid NOx levels. Reduced wound fluid NOx during impaired wound closure was, in turn, associated with decreased collagen accumulation, 34 wound tensile strength, type I and III collagen gene expression, 35 VEGF expression, granulation tissue formation, and wound microvascular perfusion.…”

Section: Discussionmentioning

confidence: 99%

Hyperbaric Oxygen Therapy Mediates Increased Nitric Oxide Production Associated With Wound Healing

Boykin

Baylis

2007

Advances in Skin &Amp; Wound Care

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Objective-The objective of this preliminary study was to document general somatic and wound nitric oxide (NO) levels during and after hyperbaric oxygen therapy (HBOT).Design-The study evaluated 6 chronic wound patients that responded favorably to HBOT treatment (20 treatments; 2.0 atmosphere absolute [ATA] × 90 minutes). Successful HBOT was associated with increased wound granulation tissue formation and significantly improved wound closure. Wound fluid and fasting plasma samples were obtained for measurement of nitrate and nitrite (NOx), the stable oxidation products of NO; plasma L-arginine (L-Arg); and asymmetric dimethylarginine (ADMA). NOx measurements were obtained before treatment (baseline), after 10 and 20 treatments, and at 1 and 4 weeks after therapy.Results-Wound fluid NOx levels tended to increase during treatments, were significantly elevated at 1 and 4 weeks after therapy, and correlated with reductions in wound area. Plasma L-Arg and ADMA were unchanged during and after HBOT.Conclusion-This preliminary study documents a significant increase in local wound NO levels (by NOx measurements) after successful HBOT and suggests that this mechanism may be an important factor in promoting enhanced wound healing and wound closure associated with this therapy.Hyperbaric oxygen therapy (HBOT) accelerates the healing of chronic wounds and is now primarily used as an adjunctive therapy in managing selected problem wound healing. [1][2][3][4] Hyperoxia during HBOT is an important mediating factor for wound collagen deposition and cross-linking, 5 neutrophil-dependent microbial killing, 6 and neovascularization. 7 Other HBOT-mediated processes in wound healing include the inhibition of integrin-mediated intravascular leukocyte adhesions and platelet aggregation, 8 enhancement of cutaneous microvascular homeostasis after ischemia-reperfusion syndrome, 9 increased oxygen capacitance and survival of ischemic flaps, 10 and upregulation of platelet-derived growth factor (PDGF) receptor mRNA. 11Experimental normobaric oxygen activates gene expression in human dermal fibroblasts, supporting the concept of oxygen as a possible signal transducer. 12 An additional signaling system modulated by oxygen availability is provided by the naturally occurring oxygen-free radicals (oxidants), or reactive oxygen species (ROS), found in the wound environment. ROS have been observed to increase cellular vascular endothelial growth factor (VEGF) expression and signal transduction that is considered to promote an overall enhancement of the wound healing process. 13 Further, accelerated wound healing during HBOT may be mediated by the combined effects of hyperoxia and the increased local (wound) production of nitric oxide (NO), an important cellular signal for tissue repair. 14,15 Ongoing experimental and clinical wound healing studies have established NO as a critical mediator of normal tissue repair. 16 Angiogenesis, granulation tissue formation, epidermal migration, collagen deposition, and microvascular homeostasis are signi...

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Section: Discussionmentioning

confidence: 99%

Hyperbaric Oxygen Therapy Mediates Increased Nitric Oxide Production Associated With Wound Healing

Boykin

Baylis

2007

Advances in Skin &Amp; Wound Care

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“…12 It is claimed that corticosterone inhibits early NOS activity. 37 NO production plays an important role in wound healing and is involved as a regulator of collagen synthesis. 16 Efron et al reported that iNOS activity in wound healing is maximal on the first day, and decreases on the third and fifth days after anastomosis.…”

Section: Discussionmentioning

confidence: 99%

Effects of triazolopyrimidine on lipid peroxidation and nitric oxide levels in the corticosteroid-impaired healing of rat tracheal anastomoses

Polat

Talaş

Polat

et al. 2004

Cell Biochem. Funct.

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Corticosteroids are used to reduce the oedema and prevent scar tissue formation of the upper airways by their ability to inhibit influx of inflammatory cells, limit capillary permeability and block collagen synthesis in the early stages of wound healing. Triazolopyrimidine (Trapidil) is an antiplatelet agent that acts in part as a phosphodiesterase inhibitor and as a competitive inhibitor of the platelet-derived growth factor (PDGF) receptor. Trapidil, with its vasodilator and NO releasing effect may have some potential to diminish the tissue injury. This study was carried out to evaluate the effects of trapidil (triazolopyrimidine) on lipid peroxidation and nitric oxide in the corticosteroid-impaired healing of tracheal anastomoses. Thirty-four adult Wistar rats were divided into five groups. The animals underwent tracheal transection and primary anastomoses. The groups were assigned as follows: group I, control, (GI, n = 6); group II, sham, (GII, n = 6); group III, dexamethasone, 0.1 mg kg(-1) twice daily intramuscularly, (GIII, n = 8); group IV, trapidil, 6 mg kg(-1) twice daily intraperitoneally (GIV, n = 7); group V, dexamethasone, 0.1 mg kg(-1) plus trapidil, 6 mg kg(-1) twice daily (GV, n = 7), for 1 week. After 1 week, anastomotic healing was assessed by measurement of bursting pressure, evaluation of histopathology, measurement of MDA and nitrite/nitrate levels. In GIII, GIV and GV bursting pressures resulted in significantly reduced anastomotic strength compared to the controls (p < 0.001 for all groups). The difference between bursting pressures of GIII and GIV was not found to be statistically significant (p = 0.966). In regard to fibroblast proliferation and collagen content, a significant difference was found between GIII and GI (p < 0.01), A significant difference was also found when GIV and GV were compared to GIII (p < 0.01). MDA and nitrite/nitrate levels were found to be higher in GIII when compared to all other groups. MDA levels of GIV and GV rats were found to be lower than GIII (p < 0.001, for both groups). The nitrite/nitrate levels of GIV and GV rats were found to be lower than GIII (p < 0.05), and higher than GI (p < 0.001). Trapidil may be useful for its preventive effects on lipid peroxidation and possible increases in NO in cases with corticosteroid-impaired healing of trachea anastomoses.

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“…As the bending wavenumbers are not much different from the expected range, the linear distortion in this case is very much greater than the angular distortion. The band due to the C-O(H) stretching vibration is generally strong and it occurs in the region 1190-1075 cm 1 . The C-O(H) stretching mode is observed as a medium-intensity band at 1191 cm 1 in the Raman spectrum and as a shoulder peak at 1181 cm 1 in the IR spectrum.…”

Section: Vibrations Of the Oh Groupmentioning

confidence: 99%

“…Owing to the in-plane vibration of the O-C O group, these bands are not usually well resolved in the region 665-610 cm 1 . In addition, a strong band is found at 555-520 cm 1 , which is attributed to the in-plane vibration of the C-C O group.…”

Section: Vibrations Of the Oc O And CC O Groupsmentioning

confidence: 99%

“…L-Arginine and L-ornithine have the physiological role of stimulating the pituitary gland to release natural growth hormone, a supplement of which may speed up wound healing. 1,2 In burn, trauma and surgical patients, muscle wasting is reduced, new muscle growth is increased and wound healing is promoted by ornithine˛-ketoglutarate therapy. 3,4 The inhibition of human ornithine decarboxylase activity by enantiomers of difluoromethylornithine has already been studied.…”

Section: Introductionmentioning

confidence: 99%

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Vibrational spectra of bis(L-ornithinium) chloride nitrate sulfate

Ramaswamy

Rajaram

Ramakrishnan

2005

J. Raman Spectrosc.

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The Raman and infrared absorption spectra of 2(C 5 H 14 N 2 O 2 2+ )·Cl − ·NO 3 − ·SO 4 2− crystal containing three anions were recorded at room temperature and were interpreted in the light of crystal structure data. The presence of a carbonyl group was identified. The carboxylic group was found to exist as COOH. The formation of O -H· · ·O, N -H· · ·O and N -H· · ·Cl asymmetric hydrogen bonds contributes considerably to the crystal cohesion and is responsible for the changes in the position and intensity of several bands. The vibrational spectra show that the anions were found to coordinate through hydrogen bonding interactions to other ligands in the crystal. The lattice wavenumbers of the halide radical (chlorine anion) were also assigned in terms of hydrogen bond vibrations.

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Altered Wound Arginine Metabolism by Corticosterone and Retinoic Acid

Cited by 33 publications

References 20 publications

Hyperbaric Oxygen Therapy Mediates Increased Nitric Oxide Production Associated With Wound Healing

Hyperbaric Oxygen Therapy Mediates Increased Nitric Oxide Production Associated With Wound Healing

Effects of triazolopyrimidine on lipid peroxidation and nitric oxide levels in the corticosteroid-impaired healing of rat tracheal anastomoses

Vibrational spectra of bis(L-ornithinium) chloride nitrate sulfate

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