Alternate Use of Different Rica Probiotic Bacteria on Survival Rate and Production of Cultured Tiger Shrimp in Semi-Intensive Ponds

Abstract

Tiger shrimp diseases have occured in Indonesian shrimp ponds for more than two decades. In order to overcome this problem, five types of the RICA probiotic bacteria were tested in nine 250 m2 semi-intensive ponds of the Research and Development Institute for Coastal Aquaculture Marana Station. In the present study three different alternate use of the RICA probiotics were tested for tiger shrimp culture incompletely randomized design experiment. There were three treatments here namely: A) alternate use of probiotic bacteria RICA-1, RICA-2, RICA-3; B) alternate use of probiotic bacteria RICA-4, RICA-5, RICA-3, and C) control (without probiotic bacteria); each treatment were applied in three replications. The results showed that survival rate and production of tiger shrimp in treatment A (55.8% and 14.9 kg/pond) and B (52.7% and 16.7 kg/pond) were significantly better (P<0.05) than those of in control ponds (37.4% and 10.9 kg/pond). However, survival rate and production of tiger shrimp between treatment A and B were not significantly different (P>0.05). The average total bacteria population in the cultured water media of treatment A (4.32 x 104 cfu/mL) and B (5.18 x 104 cfu/mL) were relatively higher than control (1.46 x 104 cfu/mL). However the percentage ratio of Vibrio spp. and total bacteria population in the cultured water media of treatment A and B were relatively lower than control. The lower survival rate and production of tiger shrimp in control (C) compared to probiotic treatments (A and B) were not just affected by the increase of Vibrio spp. ratio, but also affected by the increase of total organic matter and nitrite concentrations in the control ponds that were relatively higher than treatment A and B.

PERFORMANCE OF CULTURED White-leg Shrimp IN RICA Probiotic Application METHOD in PONDS AERATED WITH SUPERCHARGE BLOWER

“…When TBV/TPC ratio is increase more than 10%, some pathogenic Vibrio spp. might be harmful to the shrimp (Atmomarsono & Nurbaya, 2014).…”

“…Actually TBV in the control shrimp pond water did not increase much, but TBV/TPC ratio increased to 17.48% since TPC in the control shrimp pond water decreased. Atmomarsono & Nurbaya (2014) also reported that percentage of TBV/TPC ratio in the shrimp pond water over than 10% was dangerous for the cultured shrimp.…”

“…However this number could be minimized to less than 10 3 cfu/mL by applying certain appropriate RICA probiotics especially RICA1 (Brevibacillus laterosporus) or RICA3 (Pseudoalteromonas sp. Edeep-1) (Atmomarsono & Nurbaya, 2014). This RICA3 probiotic could protect the growth of Vibrio spp.…”

Use of Different Probiotics for Prevention of Vibriosis Disease on Tiger Shrimp Larvae Reared in Fiberglass Tanksuse of Different Probiotics for Prevention of Vibriosis Disease on Tiger Shrimp Larvae Reared in Fiberglass Tanks

“…One way to solve this problem is using probiotic to prevent shrimp diseases (Poernomo, 2004;Waston et al, 2008). The main culprit of shrimp diseases is vibriosis caused by Vibrio harveyi which could wipe out 100% of the cultured shrimp (Flegel, 2012;Atmomarsono & Nurbaya, 2014). As an opportunistic pathogen, Vibrio harveyi will only become pathogenic bacteria causing shrimp diseases when the pond water quality is poor (Atmomarsono & Susianingsih, 2015).…”

“…Probiotic bacteria are non-pathogenic bacteria usually used to protect the shrimp from pathogenic microorganisms, inhibit the growth of pathogenic bacteria, neutralize poor pond water quality such as organic matter, H 2 S, NH 3 -N, and NO 2 -N, and function as food (Poernomo, 2004;Wang et al, 2008). Probiotic bacteria can be isolated from sea sediment, pond sediment, mangrove leaves, and macroalgae (Atmomarsono et al, 2009;Susianingsih et al, 2012;Tampangallo et al, 2013;Atmomarsono & Nurbaya, 2014). There are various types of domestically produced and imported probiotics available in the market.…”