Alternative carbohydrates promote differentiation of plant cells

Kinnersley, Alan M.; Henderson, William Edwards

doi:10.1007/bf00039884

Cited by 25 publications

(13 citation statements)

References 18 publications

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“…Sucrose at 60 g l -1 gave the longest embryo (0.99 cm), compared to the other treatments followed by date syrup "Dibs" at 2 and 6% with significantly different. These results were in agreement with those obtained by some investigators [12,14] . Regarding fresh weight, data presented in Fig.…”

Section: Resultssupporting

confidence: 94%

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Comparison Effects of Sucrose and Date Palm Syrup on Somatic Embryogenesis of Date Palm (Phoenix dactylifera L)

Al-Khateeb¹

2008

American J. of Biochemistry and Biotechnology

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Abstract:The effect of different concentration of date palm syrup (2, 4, 6, 8 and 10%) and sucrose at concentration of 30 and 60 g/l in addition to the control (without carbon source) on the micro propagation of date palm "cv. Suckary" were investigated. The results indicated that date syrup was taken up from the media as shown by the increase in total dry weight of culture. Addition of sucrose at 60 g/l produced the highest number of somatic embryos and longest shoot equal to that produced by date syrup at concentration of 6% without significant differences. On the other side, sucrose at 30 g/l improved the germination of somatic embryos followed by date syrup at 6%. Regarding fresh and dry weights, adding 6% date palm syrup was superior in this respect. Tissues grown on medium supplement with 10% of date syrup caused severe reduction in number of somatic embryo possibly due to the osmotic stress. In addition, tissues grown on medium containing 2% of date syrup had the lowest number of embryos. Date palm tissues are capable of utilizing date syrup as the sole carbon source for vegetative growth. It can be concluded that, adding date syrup locally known as 'Dibs' to the culture medium of date palm at concentration of 6% can be used totally as a replacement of sucrose which was the normally sugar used in most of plant tissue culture medium. This is the first report on the use of date syrup on somatic embryogenesis of date palm cv. Suckary.

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Section: Resultssupporting

confidence: 94%

“…Kinnersley and Henderson [12] found that addition of corn (maize) syrup to basic culture media improves embryogenesis of wild carrot. In a comparison of the effects of adding coconut water, banana extract, pineapple juice or tomato juice to the Nitsch medium.…”

Section: Introductionmentioning

confidence: 99%

Comparison Effects of Sucrose and Date Palm Syrup on Somatic Embryogenesis of Date Palm (Phoenix dactylifera L)

Al-Khateeb¹

2008

American J. of Biochemistry and Biotechnology

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“…From the previous studies of Asemota et al (2007) they found that callus of date palm explants was initiated at 5 mg -l NAA from leaf explant when cultured in the Eeuwen's medium supplemented with NAA from 5-20 mg -l . Data presented here showed that, conversion of original somatic embryos enhanced strongly by added pollen palm extract without addition of NAA and BA (T4 and T3 treatments) this data insures the previous data obtained by Chen et al (2005) and Amo-Marco and Picazo (1994) who proved that adding plant extracts juice of coconut, tomato, potato, onion, banana, orange, apple, pineapple and yeast to the culture medium enhanced the growth of tissues, furthermore addition of corn (maize) syrup to basic culture media improves embryogenesis of wild carrot (Kinnersley and Henderson 1988). Use of other natural supplements like tomato juice, orange juice, malt extract, yeast extract, casein hydrolysate in the media for promoting multiplication and development of in vitro cultures have been demonstrated by many authors (Carimi et al, 1998on Citrus, Rahman et al, 2004and Aktar et al, 2007.…”

Section: Embryo Lengthsupporting

confidence: 89%

Inducing Date Palm Somatic Embryogenesis and Plantlets Growth Using Date Palm Pollen Grains Extract

Darwesh¹,

El-Din²,

Zayed³

et al. 2015

NIDOC-ASRT

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HIS STUDY aimed to evaluate the effect of date palm pollen grains extract contents on date palm (Phoenix dactylifera) cv. Sewi growing in vitro. This experiment was done during somatic embryos germination stage and the elongation of complete plantlets. The somatic embryos and complete plantlets were cultured on M urashige and Skoog basal medium. The treatments were, T0: M S + 0.1 mg l -1 NAA and 0.5 mg l -1 BA, T1: T0 + 200 ppm date palm pollen grains extract, T2: T0 + 400 ppm date palm pollen grains extract. T3: M S + 200 ppm date palm pollen grains extract and T4: M S +400 ppm date palm pollen grains extract. The obtained results showed that, treatment with date palm pollen grains extract at 400 ppm (T4) significantly increased germination percentage up to 70%, followed by T3 treatment (50%). While, the secondary embryos formation percentage was enhanced withT2 treatment. Concerning the average number of leaves and roots it was significantly increased with T1. The highest value of shoot length was recorded with T4. Also, there was recurrent secondary embryos formation during the elongation stage, T2 medium gave significant increases in number of secondary embryos. Chemical analyses of samples that were taken from tissues during this study refer that, newly formed secondary somatic embryos contained the highest level of protein when originally somatic embryos were cultured on T2 treatment. Complete plantlets cultured on T2 treatment contained the highest levels of total indoles, chlorophyll a, b. While the carotenoids concentrations were increased within plantlets cultured on T4 treatment. These results cleared the stimulants effects of palm pollen grains extract on the morphological and chemical estimations of date palm growing in vitro.

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“…The origin of cultures of wild carrot (Daucus carota L.), cell line WC 63-2, and the culture media used to study anthocyanin accumulation and embryogenesis has been described by Kinnersly & Henderson [8]. The same reference describes the origin and culture conditions for suspension cultures of Nicotiana tabacum cv.…”

Section: Culturesmentioning

confidence: 99%

Corn starch as an alternative gelling agent for plant tissue culture

Henderson¹,

Kinnersley²

1988

Plant Cell Tiss Organ Cult

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Growth and differentiation of plant cell cultures was increased when media were gelled with corn starch instead of agar. Dry weight of tobacco and wild carrot cell cultures on media gelled with starch was more than three times that of cultures on media gelled with agar. Higher yield of anthocyanin and dry weight of embryos were found in wild carrot cultures grown on media gelled with corn starch. The starch-mediated increase in growth and differentiation of wild carrot ceils was accompanied by an increase in density of the cultures shown by higher dry weight/fresh weight ratios.

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Alternative carbohydrates promote differentiation of plant cells

Cited by 25 publications

References 18 publications

Comparison Effects of Sucrose and Date Palm Syrup on Somatic Embryogenesis of Date Palm (Phoenix dactylifera L)

Comparison Effects of Sucrose and Date Palm Syrup on Somatic Embryogenesis of Date Palm (Phoenix dactylifera L)

Inducing Date Palm Somatic Embryogenesis and Plantlets Growth Using Date Palm Pollen Grains Extract

Corn starch as an alternative gelling agent for plant tissue culture

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