Corn starch as an alternative gelling agent for plant tissue culture

Henderson, William Edwards; Kinnersley, Alan M.

doi:10.1007/bf00039885

Cited by 69 publications

(27 citation statements)

References 7 publications

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“…Starch did not improve the regeneration rate in this investigation, in contrast to other studies, where starch improved the regeneration rate in barley anther culture (Kuhlmann & ForoughiWehr 1989;Sorvari 1986a;Sorvari & Schieder 1987), of potato tuber discs (Sorvari 1986b), and of tobacco and carrot explants (Henderson & Kinnersley 1988).…”

Section: Discussioncontrasting

confidence: 54%

“…As demonstrated in this paper, this modification resulted in a higher embryo production in the medium with potato starch compared to the medium with Gelrite only, throughout the entire culture period. Starch has been compared to agar (Henderson & Kinnersley 1988;Sorvari 1986a,b;Sorvari & Schieder 1987) and its stimulating effect in tissue culture is often explained by the absence of inhibitory substances, which are present in agar (Johansson 1983;Kohlenbach & Wernicke 1978). In our investigation, we compared starch with Gelrite, an inert gel, which Johansson (1988) had shown to be superior to agar for shoot multiplication of potato.…”

Section: Discussionmentioning

confidence: 99%

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The effect of starch and ineubation temperature in anther culture of potato

Calleberg

Johansson

1993

Plant Cell Tiss Organ Cult

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Three Andean tetraploid potato genotypes (2n= 48) and 7 anther-derived dihaploids (2n=24) originating from two of the tetraploids were used in anther culture. Relative number of embryos/vial was significantly higher when the anther culture media was gelatinized with 3% potato starch than when Gelrite or wheat starch (3%) were used as gelatinizing agents. The degree of anther culture response varied between tetraploids but also within a group of related dihaploids. Additionally, the embryo production of individual genotypes, tetraploids as well as dihaploids, was dependent on the incubation temperature (10, 15, 20, 25, 30°C) of the anther culture. The incubation temperature of the anther culture was also important for the regeneration rate. Direct regeneration was mostly stimulated when the anther culture was incubated at 20°C.

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Section: Discussioncontrasting

confidence: 54%

Section: Discussionmentioning

confidence: 99%

The effect of starch and ineubation temperature in anther culture of potato

Calleberg

Johansson

1993

Plant Cell Tiss Organ Cult

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“…Agar is still one of the most used gelling agents to solidify media for plant tissue cultures, because it has several advantages (George & Sherrington 1984). The properties of agar like stability, clarity, non-toxic nature and resistance to metabolism during culture decide the choice of gelling agent (Henderson & Kinnersley 1988). In spite of wide application, the most important limitation of agar is its standard (Debergh 1983) and cost.…”

Section: Discussionmentioning

confidence: 99%

Assessment of factors on shoot proliferation potential of nodal explants of Phyllanthus fraternus and assessment of genetic fidelity of micropropagated plants using RAPD marker

et al. 2014

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Phyllanthus fraternus is an important medicinal plant, popularly known for its hepatoprotective and antiviral activities since ancient times. Various physiological factors like carbon sources, concentration of agar, pH of the media and effect of season of explants collection were optimized for high frequency regeneration of P. fraternus. The frequency of regeneration, average number and length of shoots were highly influenced by the type and concentration of carbon sources (monosaccharides and disaccharides, 1 to 4%), agar concentration (0.2 to 1%) and pH (4.5 to 6.8) of the media. Media containing 3% sucrose, 0.6% agar at pH 5.8 was best for regeneration. Seasonal variation of explants collection significantly affected the axillary shoots proliferation from explants and best proliferation was observed from explants collected during April to June. Genetic fidelity of regenerated plants was assessed by random amplified polymorphic DNA markers. No polymorphism was detected in micropropagated plants and the mother plant, revealing the genetic homogeneity of the in vitro raised plantlets. This is the first report regarding establishment of genetic fidelity of micropropagated P. fraternus plants, which could be successfully applied for the mass multiplication, germplasm conservation and further genetic transformation assays to meet the ever increasing demand of this medicinally potent plant for industrial and pharmaceutical uses.

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“…In a medium solidified with starch, cell dry weight increased more than three times with respect to cells grown in a medium gelled with agar [24].…”

Section: Alternative Gelling Agentsmentioning

confidence: 92%

Polyamines, Gelling Agents in Tissue Culture, Micropropagation of Medicinal Plants and Bioreactors

Lima¹,

Campos²,

Willadino³

et al. 2012

Recent Advances in Plant in Vitro Culture

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Currently, tissue cultures of species of agricultural importance have wide applicability in industrial production processes. Tissue culture is a name given to a set of techniques that allow the regeneration of cells, tissues and organs of plants, from segments of plant organs or tissues, using nutrient solutions in aseptic and controlled environment. This regeneration is based on the totipotency of plant cells. Totipotency is a capability indicating that plant cells, in different times, may express the potential to form a new multicellular individual. Tissue culture appears to be a good alternative to conventional propagation, requiring less physical space, with high multiplication rate, without incidence of pests and diseases during cultivation, and enabling higher control of the variables involved. Thus, in the in vitro environment, with the required stimuli and appropriate conditions, different cell types express different behaviors, possibly leading to cell multiplication and differentiation into a specific tissue, characterized by a form and a function, which may lead to the regeneration of a new individual

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Corn starch as an alternative gelling agent for plant tissue culture

Cited by 69 publications

References 7 publications

The effect of starch and ineubation temperature in anther culture of potato

The effect of starch and ineubation temperature in anther culture of potato

Assessment of factors on shoot proliferation potential of nodal explants of Phyllanthus fraternus and assessment of genetic fidelity of micropropagated plants using RAPD marker

Polyamines, Gelling Agents in Tissue Culture, Micropropagation of Medicinal Plants and Bioreactors

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