The effect of starch and ineubation temperature in anther culture of potato

Abstract

Three Andean tetraploid potato genotypes (2n= 48) and 7 anther-derived dihaploids (2n=24) originating from two of the tetraploids were used in anther culture. Relative number of embryos/vial was significantly higher when the anther culture media was gelatinized with 3% potato starch than when Gelrite or wheat starch (3%) were used as gelatinizing agents. The degree of anther culture response varied between tetraploids but also within a group of related dihaploids. Additionally, the embryo production of individual genotypes, tetraploids as well as dihaploids, was dependent on the incubation temperature (10, 15, 20, 25, 30°C) of the anther culture. The incubation temperature of the anther culture was also important for the regeneration rate. Direct regeneration was mostly stimulated when the anther culture was incubated at 20°C.

Somatic Embryogenesis for Potato (Solanum tuberosum L.) Improvement

“…Parthenogenesis can occur when a paternal nucleus fertilizes the polar nuclei but the egg cell is not fertilized. Parental haploids are obtained using male gametes via anther or microspore culture (Uhrig and Salamini 1987;Calleberg and Johansson 1993;Rokka et al 1998). De Maine (1995) examined effects of a second haploid generation on inbreeding of somatically chromosome-doubled (homozygous dihaploid) potato from crosses with S. phureja clones.…”