Somatic Genome Manipulation

Li, Xiuqing; Donnelly, Danielle J.; Jensen, Thomas G.

doi:10.1007/978-1-4939-2389-2

2015

DOI: 10.1007/978-1-4939-2389-2

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Somatic Genome Manipulation

Xiuqing Li¹,

Danielle J. Donnelly²,

Thomas G. Jensen³

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Cited by 5 publications

References 105 publications

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Generation of c-MycERTAM-transduced human late-adherent olfactory mucosa cells for potential regenerative applications

Santiago-Toledo

Georgiou

Reis

et al. 2019

Sci Rep

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Human olfactory mucosa cells (hOMCs) have been transplanted to the damaged spinal cord both pre-clinically and clinically. To date mainly autologous cells have been tested. However, inter-patient variability in cell recovery and quality, and the fact that the neuroprotective olfactory ensheathing cell (OEC) subset is difficult to isolate, means an allogeneic hOMC therapy would be an attractive “off-the-shelf” alternative. The aim of this study was to generate a candidate cell line from late-adherent hOMCs, thought to contain the OEC subset. Primary late-adherent hOMCs were transduced with a c-MycERTAM gene that enables cell proliferation in the presence of 4-hydroxytamoxifen (4-OHT). Two c-MycERTAM-derived polyclonal populations, PA5 and PA7, were generated and expanded. PA5 cells had a normal human karyotype (46, XY) and exhibited faster growth kinetics than PA7, and were therefore selected for further characterisation. PA5 hOMCs express glial markers (p75NTR, S100ß, GFAP and oligodendrocyte marker O4), neuronal markers (nestin and ß-III-tubulin) and fibroblast-associated markers (CD90/Thy1 and fibronectin). Co-culture of PA5 cells with a neuronal cell line (NG108-15) and with primary dorsal root ganglion (DRG) neurons resulted in significant neurite outgrowth after 5 days. Therefore, c-MycERTAM-derived PA5 hOMCs have potential as a regenerative therapy for neural cells.

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Generation of c-MycERTAM-transduced human late-adherent olfactory mucosa cells for potential regenerative applications

Santiago-Toledo

Georgiou

Reis

et al. 2019

Sci Rep

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The relation between apomictic seed production and morpho-physiological characteristics in a world collection of castor bean (Ricinus communis L.)

Setayeshnasab,

Sabzalian,

Rahimmalek

2024

Sci Rep

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Ricinus communis is one of the most important oilseed plants with many medicinal and industrial applications. Variation in 30 genotypes of castor bean collected from different regions of the world was evaluated for two consecutive years and the difference in seed production with two different reproductive modes (including apomixis and open-pollination) was compared based on yield components, agronomic traits, and phytochemical properties. Results of data analysis demonstrated that castor bean has the ability for a wide range of apomixis for seed production and the highest percentages of apomixis ability in the first and second years were 86.3% and 92.31%, respectively. Apomixis ability had a high positive correlation with yield components, seed oil content, and the amount of leaf rutin. Two genotypes from Brazil and Syria revealed the highest phenolic content in the first and second years, respectively. In addition, the Afghanistan genotype in two modes of apomixis and open-pollination in the first year and the Syria and Yazd genotypes in apomixis and open-pollination modes, respectively, in the second year showed the highest content of seed fatty acids. It is possible to maintain superior genotypes of castor bean in terms of phytochemical traits, yield, and oil quality through apomixis reproduction.

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Mechanism and Utilization of Ogura Cytoplasmic Male Sterility in Cruciferae Crops

Ren

Chen

et al. 2022

IJMS

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Hybrid production using lines with cytoplasmic male sterility (CMS) has become an important way to utilize heterosis in vegetables. Ogura CMS, with the advantages of complete pollen abortion, ease of transfer and a progeny sterility rate reaching 100%, is widely used in cruciferous crop breeding. The mapping, cloning, mechanism and application of Ogura CMS and fertility restorer genes in Brassica napus, Brassica rapa, Brassica oleracea and other cruciferous crops are reviewed herein, and the existing problems and future research directions in the application of Ogura CMS are discussed.

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Somatic Genome Manipulation

Cited by 5 publications

References 105 publications

Generation of c-MycERTAM-transduced human late-adherent olfactory mucosa cells for potential regenerative applications

Generation of c-MycERTAM-transduced human late-adherent olfactory mucosa cells for potential regenerative applications

The relation between apomictic seed production and morpho-physiological characteristics in a world collection of castor bean (Ricinus communis L.)

Mechanism and Utilization of Ogura Cytoplasmic Male Sterility in Cruciferae Crops

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