Alternative formulations of E. scaber and S. androgynous extracts as immunomodulatory agents for suppression of bacterial infection in pregnant mice: A case of traditional herbal formulas in reproduction

Djati, Muhammad Sasmito

doi:10.1063/1.5061908

Cited by 1 publication

(1 citation statement)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…typhi infection. The combination of both plant extract lead to synergistic effects in stimulating the immune system by modulating IL-2 secretion [ 47 , 48 ].…”

Section: Discussionmentioning

confidence: 99%

Hormone-balancing and protective effect of combined extract of Sauropus androgynus and Elephantopus scaber against Escherichia coli-induced renal and hepatic necrosis in pregnant mice

Christina

Diana

Fuzianingsih

et al. 2021

Journal of Ayurveda and Integrative Medicine

Self Cite

View full text Add to dashboard Cite

Background Elephantopus scaber (ES) and Sauropus androgynous (SA) have been frequently reported to possess antibacterial activity through in vitro, but in vivo studies about the protective effect of combined ES and SA have acquired less attention. Objectives To evaluate protective effect of combined ethanol extract of ES and SA on hormone imbalance and renal and hepatic necrosis formation in Escherichia coli -infected pregnant mice. Materials and methods A total of 28 pregnant Balb/c mice were divided into seven groups (n = 4): control, E. coli -infected pregnant mice, infected pregnant mice received 200 mg/kg ES, infected pregnant mice received combined 150 mg/kg ES and 37.5 mg/kg SA (75:25), 100 mg/kg ES and 75 mg/kg SA (50:50) , 50 mg/kg ES and 112.5 mg/kg SA (25:75), and only 150 mg/kg SA. Pregnant mice were orally treated with combined ES and SA on day 1–4th of pregnancy. On the 4th day, mice were infected with 10 7 CFU/mL of E. coli and continuously treated with ES and SA until the 16th day of pregnancy. After treatment, the kidney and liver were prepared for histological examination using H&E staining. The blood serum was collected in each stage of pregnancy and measured by ELISA assays. Results Combined ES and SA gave an impact on altering the prolactin level. Combined ES and SA at ratio dose 75:25 was able to restore progesterone to normal levels (P < 0.05). The level of estradiol (E2) was relatively stable in the presence of E. coli and treatment. Treatment with 200 mg/kg ES, combined 50 mg/kg ES and 112.5 mg/kg SA (25:75) and 100 mg/kg ES and 75 mg/kg SA (50:50) demonstrated an immunomodulatory effect on the Gr1 + cell of E. coli treated-pregnant mice. E. coli infection significantly increased renal tubules and hepatic necrosis in pregnant mice compared to control (P < 0.05). Combined SA and ES at ratio dose 75:25 significantly demonstrated remarkable renal and hepatic protection activity in infected pregnant mice. Conclusion The present study provided the establishment of combined ES and SA could be used to invent potent hormonal balancing agent and hepato-renal protective agent in infected pregnant mice.

show abstract

“…typhi infection. The combination of both plant extract lead to synergistic effects in stimulating the immune system by modulating IL-2 secretion [ 47 , 48 ].…”

Section: Discussionmentioning

confidence: 99%