Alternative haematotoxicological testing

Berger, Josef

doi:10.2478/v10136-009-0003-y

Cited by 8 publications

(4 citation statements)

References 41 publications

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“…New alternative models for toxicological safety tests of chemicals have been found to achieve faster, cheaper and more accurate preclinical prediction (Asami et al 2010, Benigni et al 2010, Berger 2010, Gadea and Garcia-Vazquez 2010. As toxicology leans towards the acquisition of knowledge of safety tests before the investigation of mechanisms, the evaluation of known drugs and poisons still predominates among recent studies (cf.…”

Section: Drug and Poison Researchmentioning

confidence: 99%

“…Computational toxicology supports physiologically based pharmacokinetic and pharmacodynamic modelling, quantitative structure-activity relationship methods, and dose models (Rusyn andDaston 2010, Valerio 2010). In silico models have been developed for drug and vaccine screening (Berger 2010, Naven et al 2010, Sung et al 2010, cancers (Edelman et al 2010), and studies of blood coagulation proteins (Villoutreix and Sperandio 2010).…”

Section: Computational Medicinementioning

confidence: 99%

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The age of biomedicine: current trends in traditional subjects

Berger¹

2011

J Appl Biomed

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The earliest scientific journals on biomedicine began publication in the 50s and their authors addressed the application of biology to medicine. More recently, biochemistry and biomedical engineering questions have figured more prominently. This trend is discussed in a survey of the topics appearing in the Journal of Applied Biomedicine. Pharmacological and toxicological articles have been popular over the long term and the neurosciences, chronomedicine, molecular and cell biomedicine have also been very important. The role of computational biomedicine and nanomedicine has received increasing attention as has the part which applied biomedicine can play in the enhancement of the general economy.

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Section: Drug and Poison Researchmentioning

confidence: 99%

Section: Computational Medicinementioning

confidence: 99%

The age of biomedicine: current trends in traditional subjects

Berger¹

2011

J Appl Biomed

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“…Large insect larvae can be easily injected into the midgut or subcutaneously with sample solution using a syringe. Silkworm hemolymph and tissue can be harvested separately and used in biochemical, haematological, and immunological analyses [7,8]. Thus, the silkworm is an invertebrate model that can relieve the issues related to the use of mammals and thus promote pharmaceutical studies [7,9-12].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of drug-induced tissue injury by measuring alanine aminotransferase (ALT) activity in silkworm hemolymph

Inagaki

Matsumoto

Kataoka

et al. 2012

BMC Pharmacol Toxicol

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BackgroundOur previous studies suggest silkworms can be used as model animals instead of mammals in pharmacologic studies to develop novel therapeutic medicines. We examined the usefulness of the silkworm larvae Bombyx mori as an animal model for evaluating tissue injury induced by various cytotoxic drugs. Drugs that induce hepatotoxic effects in mammals were injected into the silkworm hemocoel, and alanine aminotransferase (ALT) activity was measured in the hemolymph 1 day later.ResultsInjection of CCl4 into the hemocoel led to an increase in ALT activity. The increase in ALT activity was attenuated by pretreatment with N-acetyl-L-cysteine. Injection of benzoic acid derivatives, ferric sulfate, sodium valproate, tetracycline, amiodarone hydrochloride, methyldopa, ketoconazole, pemoline (Betanamin), N-nitroso-fenfluramine, and D-galactosamine also increased ALT activity.ConclusionsThese findings indicate that silkworms are useful for evaluating the effects of chemicals that induce tissue injury in mammals.

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“…Both linden bug (Pyrrhocoris apterus) adults and cotton leaf worm (Spodoptera littoralis) larvae are used as "laboratory" insects in physiology research, and their insectaria are standardized in ways similar to the more well-known rodent vivaria (Berger 2009a, Picmonova andBerger 2012). Their haemocyte morphology has been described and these species are suggested as applicable biomodels for toxicological screening elsewhere (Gelbič et al 2006, Berger and Slavíčková 2008, Berger 2009b, 2010. Nevertheless, the functional characteristics of haemocytes in these two insect species are unknown.…”

Section: Introductionmentioning

confidence: 99%

Phagocytosis of insect haemocytes as a new alternative model

Berger¹,

Jurčová²

2012

J Appl Biomed

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Phagocytosis is an important function of both insect haemocytes and mammalian blood cells. Linden bugs and cotton leaf worms have been suggested as new alternative models for ecological and drug toxicology but no data on their haemocyte physiology have been published. Our assays with particle ingestion of the NBT test were carried out on prohaemocytes, granulocytes, plasmatocytes and spherulocytes of adult linden bug and cotton leaf worm larvae. We found that phagocytic activity is on average 10% in the linden bug, and 50% in cotton leaf worm haemocytes: the phagocytic index is 3.5 in both species and nitroblue tetrazolium reduction is 0.5 in the linden bug and 3.2 in the cotton leaf worm. Phagocytic charactersitics of the prohaemocytes and granulocytes in the cotton leaf worm are closed to mammalian neutrophil physiology. Our data suggest that cotton leaf worm haemocytes may be a new potential alternative model for screening of phagocytosis.

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Alternative haematotoxicological testing

Cited by 8 publications

References 41 publications

The age of biomedicine: current trends in traditional subjects

The age of biomedicine: current trends in traditional subjects

Evaluation of drug-induced tissue injury by measuring alanine aminotransferase (ALT) activity in silkworm hemolymph

Phagocytosis of insect haemocytes as a new alternative model

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