Alternative induction of de novo shoot organogenesis or somatic embryogenesis from in vitro cultures of mature zygotic embryos of passion fruit (Passiflora edulis Sims) is modulated by the ratio between auxin and cytokinin in the medium

Rocha, Diego Ismael; Monte-Bello, Carolina Cassano; Dornelas, Marcelo Carnier

doi:10.1007/s11240-014-0663-5

Cited by 40 publications

(25 citation statements)

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“…In Passiflora (Passifloraceae), which is a group of tropical plants with recognized economic importance, the occurrence of SE has already been described for some species (as reviewed by Otoni et al 2013), from cell suspensions (Otoni et al 1996), protoplast cultures (Anthony et al 1999), root explants (Reis et al 2007) and mature zygotic embryos (Silva et al 2009;Paim Pinto et al 2011;Rocha et al 2012;Rocha et al 2015;Rosa et al 2015). For Passiflora edulis Sims, which is the most widely commercially cultivated passion fruit species, the culture of zygotic embryo explants with a high auxin/cytokinin ratio yields a complex embryogenic callus from which somatic embryos arise after 25-30 days of culture (Paim Pinto et al 2011;Rocha et al 2015).…”

Section: Introductionmentioning

confidence: 99%

Cellular and molecular changes associated with competence acquisition during passion fruit somatic embryogenesis: ultrastructural characterization and analysis of SERK gene expression

et al. 2015

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The integration of cellular and molecular data is essential for understanding the mechanisms involved in the acquisition of competence by plant somatic cells and the cytological changes that underlie this process. In the present study, we investigated the dynamics and fate of Passiflora edulis Sims cotyledon explants that were committed to somatic embryogenesis by characterizing the associated ultrastructural events and analysing the expression of a putative P. edulis ortholog of the Somatic Embryogenesis Receptor-like Kinase (SERK) gene. Embryogenic calli were obtained from zygotic embryo explants cultured on Murashige and Skoog medium supplemented with 2,4-dichlorophenoxyacetic acid and 6-benzyladenine. Callus formation was initiated by the division of cells derived from the protodermal and subprotodermal cells on the abaxial side of the cotyledons. The isodiametric protodermal cells of the cotyledon explants adopted a columnar shape and became meristematic at the onset of PeSERK expression, which was not initially detected in explant cells. Therefore, we propose that these changes represent the first observable steps towards the acquisition of a competent state within this regeneration system. PeSERK expression was limited to the early stages of somatic embryogenesis; the expression of this gene was confined to proembryogenic zones and was absent in the embryos after the globular stage. Our data also demonstrated that the dynamics of the mobilization of reserve compounds correlated with the differentiation of the embryogenic callus.

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Section: Introductionmentioning

confidence: 99%

Cellular and molecular changes associated with competence acquisition during passion fruit somatic embryogenesis: ultrastructural characterization and analysis of SERK gene expression

et al. 2015

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“…Auxins and cytokinins are the main PGRs involved in the regulation of plant cell differentiation, and their ratio is very critical for the specification of cell identity during early stages of morphogenesis (Fehér et al 2003;Gaj 2004;Jiménez 2,4-D was the best treatment for somatic embryogenesis induction of saffron. Rocha et al (2015) mentioned that 2,4-D was very essential to induce the callus formation and somatic embryo induction as well as plant regeneration of P. edulis. Nodal segments cultured on the medium supplemented with BA formed green and compact calluses, and shoot development from these calluses occurred on the medium containing 13.2 uM BA after 60 days of culture with the highest regeneration efficiency (Pacheco et al 2012).…”

Section: Resultsmentioning

confidence: 99%

Effect of plant growth regulators and antioxidants on in vitro plant regeneration and callus induction from leaf explants of purple passion fruit (Passiflora edulis Sims)

2017

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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.Abstract Purple passion fruit (Passiflora edulis Sims) is one of the introduced tropical plants, an increasing interest has arisen due to its distinctive taste and attractive flavor. It is expected that passion fruit production and planted area will increase gradually in the years ahead because of high profitability and consumer's demands of healthful ingredients. So we tried to investigate the effect of plant growth regulators and antioxidants on in vitro plant regeneration and callus induction from leaf explants of passion fruit for an establishment of optimal mass propagation system. Young leaf explants of purple passion fruit were cultured in Murashige and Skoog (MS) medium containing different growth regulators and antioxidant additives to induce the shoot organogenesis. After 8 weeks, the highest embryogenic callus formation rate was obtained in MS medium supplemented with 1 mg･L -1 6-benzylaminopurine (BAP) and 2 mg･L -1 2,4-dichlorophenoxyacetic acid (2,4-D), furthermore, the shoot development via organogenesis was also observed. Silver nitrate (AgNO 3 ), which was added into the medium to minimize the adverse effects of leached phenolics, was effective for reduction of medium browning and sudden explant death. In the medium supplemented with 1 mg･L -1 BAP and 1 mg･L -1 gibberellic acid (GA 3 ), shoots were most vigorously regenerated and elongated. Most shoots rooted successfully in half strength medium with 1 mg･L -1 indol-3 acetic acid (IAA), and more than 90% of plantlets survived after 4-month acclimatization period.

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“…Já o uso de embriões zigóticos é mais empregado na regeneração pela via embriogênica (SILVA et al, 2009;PINTO et al, 2011). Rocha et al (2015) utilizando-se de embriões zigóticos para a regeneração pelas vias organogênica e embriogênica relatam que este tipo de explante pode responder no desenvolvimento de brotações e/ou embriões somáticos. Além disso, as respostas são dependentes das concentrações dos reguladores de crescimento disponíveis no meio de cultivo.…”

Section: Figure 3 Direct and Indirect Organogenesis From Zygotic Embunclassified

“…As técnicas aplicadas para a regeneração de maracujazeiros por meio da cultura de tecidos aumentaram exponencialmente em todo o mundo, utilizando-se de diferentes tipos de explantes como, discos foliares em P. edulis (AMUGUNE et al, 1993), segmentos intermodais em P. edulis (BIASI et al, 2000), radiculares em P. cincinnata e P. edulis , foliares de P. alata (PACHECO et al, 2012), em P. suberosa a partir de segmentos nodais, intermodais e seguimentos foliares (GARCIA et al, 2011), explantes hipocotiledonares em P. setacea (VIEIRA et al, 2014), nodais em P. foetida (ANAND et al, 2012;SHEKHAWAT et al, 2015), hipocotiledonares e radiculares provenientes de P. edulis (ROCHA et al, 2016) e embrião zigótico (ROCHA et al, 2015).…”

Section: Introductionunclassified

Regeneração in vitro de Passiflora miniata Mast

Carvalho

Antoniazzi

Silva

et al. 2017

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RegeneRação in vitRo deRESUMO Passiflora miniata é uma espécie silvestre nativa da Amazônica Meridional, com potencial ornamental devido à beleza de suas flores de coloração vermelha intensa. Relatos na literatura sobre a espécie ainda são insipientes. O objetivo do presente estudo foi induzir a regeneração de novo pela via organogênica a partir de embriões zigóticos maduros de P. miniata. Os embriões zigóticos foram isolados e cultivados no meio de MS com a adição dos reguladores de crescimento 6-Benziladenine (BA), Thidiazuron (TDZ) e Cinetina (CIN). A regeneração de novo nos embriões zigóticos ocorreu de forma direta e/ou indireta. Um percentual de 80% dos explantes cultivados na presença de BA apresentaram organogênese pela via direta e 20% pela indireta, com TDZ 60% foram regenerados pela via direta e 40% pela indireta. Já nos tratamentos com CIN 58% do explantes apresentaram regeneração por organogênese direta e 42% pela indireta. O desenvolvimento dos primórdios das brotações iniciou-se com a formação das estruturas organogênicas que posteriormente se diferenciaram em multibrotações. As maiores médias no número de brotos produzidos foram na concentração de 0,75 mg L -1 de BA com 40,0 de brotos por explantes, sendo TDZ e CIN na concentração de 0,50 mg L -1 com média de 7,2 e 3,6, respectivamente. Palavras chaves: Passiflora miniata, Maracujá Amazônico, Embrião zigótico, Organogênese. ABSTRACT In vitro regeneration of Passiflora miniata MastPassiflora miniata is a wild species native to the Southern Amazon, with ornamental potential due to the beauty of its flowers of intense red coloration. Reports in the literature about the species are still insipid. The aim of the present study was to induce the regeneration of P. miniata by the de novo organogenesis from mature zygotic embryos. The zygotic embryos were isolated and cultivated into the MS medium with the addition of 6-Benzyladenine (BA), Thidiazuron (TDZ) and Kinetin (KIN) growth regulators. The de novo regeneration from the zygotic embryos occurred directly and indirectly. A percentage of 80% of the explants cultivated in the presence of BA had direct organogenesis and 20% by the indirect way, with TDZ 60% were regenerated by the direct and 40% by the indirect way. Regarding the treatments with KIN, 58% of the explants had regeneration by direct and 42% by the indirect organogenesis. The development of shoot primordia initiated with the formation of organogenic structures that later differentiated into multi-shoots. The highest mean number of shoots (40.0 shoots per explants) was obtained on 0.75 mg L -1 BA. Conversely, using 0.50 mg L -1 TDZ or KIN, the highest number of shoots were 7.2 and 3.6, respectively.

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Alternative induction of de novo shoot organogenesis or somatic embryogenesis from in vitro cultures of mature zygotic embryos of passion fruit (Passiflora edulis Sims) is modulated by the ratio between auxin and cytokinin in the medium

Cited by 40 publications

References 56 publications

Cellular and molecular changes associated with competence acquisition during passion fruit somatic embryogenesis: ultrastructural characterization and analysis of SERK gene expression

Cellular and molecular changes associated with competence acquisition during passion fruit somatic embryogenesis: ultrastructural characterization and analysis of SERK gene expression

Effect of plant growth regulators and antioxidants on in vitro plant regeneration and callus induction from leaf explants of purple passion fruit (Passiflora edulis Sims)

Regeneração in vitro de Passiflora miniata Mast

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