Alternative pathways of ovarian apoptosis: death for life

Amsterdam, Abraham; Sasson, Ravid; Keren-Tal, Iris; Aharoni, Dorit; Dantes, Ada; Rimon, Ephraim; Land, Allison M.; Cohen, T.; Dor, Yehoshua; Hirsh, Liron

doi:10.1016/s0006-2952(03)00485-4

Cited by 66 publications

(49 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…All of the graphs for apoptosis and necrosis were based on Q2, which indicates a combination of apoptosis and necrosis. Q1 was not reliable because the positive stain for early apoptosis may have been induced during cell preparation and therefore would not be a true indication of cell health status (Amsterdam et al 2003, Demchenko 2013). …”

Section: Apoptosismentioning

confidence: 99%

“…It is important to note that in the present study, we did not exclude any follicles on the basis of estrogen production or morphological indications of programmed DNA fragmentation, because previous findings demonstrated that the LHR binding assays revealed no differences between the follicles that were determined to be atretic and those that were deemed non-atretic in the same size class (McNatty et al 1986a). Furthermore, it has also been reported more recently that granulosa cell apoptosis (which is indicated by DNA fragmentation and cytoplasmic blebbing) can be present with functional steroidogenic mitochondria (Amsterdam et al 2003). It is, however, acknowledged that the levels of follicle apoptosis and cell DNA fragmentation are lower in antral follicles with greater estrogen production than androgen production (Yuan & Giudice 1997).…”

Section: Ovulation Rate and Apoptosismentioning

confidence: 99%

See 1 more Smart Citation

Flow cytometric analysis of FSHR, BMRR1B, LHR and apoptosis in granulosa cells and ovulation rate in merino sheep

Regan¹,

McFarlane²,

O’Shea³

et al. 2015

Reproduction

View full text Add to dashboard Cite

The aim of the present study was to determine the direct cause of the mutation-induced, increased ovulation rate in Booroola Merino (BB) sheep. Granulosa cells were removed from antral follicles before ovulation and post-ovulation from BB (nZ5) and WT (nZ12) Merino ewes. Direct immunofluorescence measurement of mature cell surface receptors using flow cytometry demonstrated a significant up-regulation of FSH receptor (FSHR), transforming growth factor beta type 1, bone morphogenetic protein receptor (BMPR1B), and LH receptor (LHR) in BB sheep. The increased density of FSHR and LHR provide novel evidence of a mechanism for increasing the number of follicles that are recruited during dominant follicle selection. The compounding increase in receptors with increasing follicle size maintained the multiple follicles and reduced the apoptosis, which contributed to a high ovulation rate in BB sheep. In addition, we report a mutation-independent mechanism of down-regulation to reduce receptor density of the leading dominant follicle in sheep. The suppression of receptor density coincides with the cessation of mitogenic growth and steroidogenic differentiation as part of the luteinization of the follicle. The BB mutation-induced attenuation of BMPR1B signaling led to an increased density of the FSHR and LHR and a concurrent reduction in apoptosis to increase the ovulation rate. The role of BMPs in receptor modulation is implicated in the development of multiple ovulations.Reproduction (2015) 150 151-163

show abstract

Section: Apoptosismentioning

confidence: 99%

Section: Ovulation Rate and Apoptosismentioning

confidence: 99%

Flow cytometric analysis of FSHR, BMRR1B, LHR and apoptosis in granulosa cells and ovulation rate in merino sheep

Regan¹,

McFarlane²,

O’Shea³

et al. 2015

Reproduction

View full text Add to dashboard Cite

show abstract

“…These morphological and biochemical changes that occur during apoptosis in ovarian follicles are similar to apoptotic changes occurring in other tissues and cells (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15).…”

mentioning

confidence: 71%

Confocal laser scanning microscopy of whole mouse ovaries: Excellent morphology, apoptosis detection, and spectroscopy

Zucker

Jeffay

2006

Cytometry Pt A

View full text Add to dashboard Cite

Background:Ovaries consist of numerous follicles, oocytes, and granulosa cells in different stages of development. Many of these follicles will undergo an apoptotic process during the lifetime of the animal. By using proper tissue preparation methods, the events within the whole ovary can be observed by using 3D confocal microscopy.Methods:Whole ovaries were stained with LysoTracker Red (LT), fixed with 4% paraformaldehyde (PF) and 1% glutaraldehyde (Glut), dehydrated with methanol (MEOH), and cleared with benzyl alcohol and benzyl benzoate (BABB). Using this tissue preparation technique, the ovary becomes relatively transparent, allowing its morphology to be observed with confocal microscopes. A spectral imaging system (PARISS) located on a conventional microscope was used to interpret the LT dye spectra and fixation products in the tissues with different excitation wavelengths.Results:Apoptosis in the follicle was detected as clusters of intensely stained granulosa cells located in close proximity to the oocytes. The fixation with Glut and PF preserved morphological details, increased tissue fluorescence, thus increased the signal to noise of the background image.Conclusions:Thick tissues can be imaged after they are properly stained, aldehyde fixed, and BABB cleared. LT intensely stained single cells or clusters of apoptotic cells in the follicles and the nucleolus. Spectral differences between LT as an indicator of apoptosis and Glut‐PF fixation was used to visualize ovarian morphology and apoptosis. The PARISS spectrophotometer revealed spectral peaks for LT at 609.6 nm and for Glut‐PF at 471.3 nm. The proper use of the spectra from these fluorescence molecules is the foundation for high quality morphological images of apoptosis. By sequentially imaging the two probes with a 488 nm laser and a 543/568 nm laser, there was a reduction in fluorescent cross talk and an increase in image quality. © 2006 International Society for Analytical Cytology

show abstract

“…33 Microarray analysis using mRNA from apoptotic cells showed that protease Granzyme B is upregulated to allow apoptotic signals to bypass mitochondrial signals, suggesting an apoptotic pathway alternative to p53. 34 Specifically, activation of serine proteases may occur downstream from the activation of caspases during apoptosis. 35 However, the link between p53 and serine proteases may not be direct, considering the data showing that DNA damage upregulates serine proteases even in p53-null HL60 cells, human leukemia cells.…”

Section: Discussionmentioning

confidence: 99%

P53-dependent induction of serine proteases in irradiated mouse colon

Lin

Cook²,

Finnberg³

et al. 2004

Cancer Biology & Therapy

View full text Add to dashboard Cite

Tumor suppressor p53 induces apoptosis through the transactivation of target genes. Previous work has shown that p53-dependent gene expression changes in response to ionizing radiation are tissue specific. To determine critical p53 target genes in the colon, we irradiated wild-type and p53-null mice and examined the global p53 gene expression patterns in response to ionizing radiation. Microarray analysis using the Affymetrix MOE430A genechip showed that many of the genes that increased in a p53-dependent manner are serine proteases (e.g., elastase, trypsin) and other proteases (e.g., carboxypeptidases). Reverse transcription polymerase chain reaction (RT-PCR), immunohistochemistry, and Western blots were used to validate microarray results on trypsin 4, carboxypeptidase A1, and elastase-2, three genes that have potential p53-binding elements. Further study of tissue specific mediators of p53-dependent responses such as serine proteases will greatly increase understanding of in vivo p53-dependent pathways within the colon triggered by ionizing radiation.

show abstract

Alternative pathways of ovarian apoptosis: death for life

Cited by 66 publications

References 44 publications

Flow cytometric analysis of FSHR, BMRR1B, LHR and apoptosis in granulosa cells and ovulation rate in merino sheep

Flow cytometric analysis of FSHR, BMRR1B, LHR and apoptosis in granulosa cells and ovulation rate in merino sheep

Confocal laser scanning microscopy of whole mouse ovaries: Excellent morphology, apoptosis detection, and spectroscopy

P53-dependent induction of serine proteases in irradiated mouse colon

Contact Info

Product

Resources

About