Abstract. We have recently succeeded in immortalizing rat granulosa cells by co-transfection with SV-40 DNA and the Ha-ms oncogene. These cells lost their response to gonadotropins, but expressed the cytochrome P450~ mitochondrial system enzymes and produced progesterone and 20ot-hydroxy-4-pregnan-3-one (20ol-OH-P) upon cAMP stimulation (Suh, B. S., and A.
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