Alternative splicing expression of U1 snRNP 70K gene is evolutionary conserved between different plant species

Abstract

A U1-snRNP--specific 70K (U1-70K) protein is intricately involved in both constitutive and alternative splicing of pre-mRNAs. Here, we report cDNA and cognate genomic sequences of the U1-70K gene of maize and rice. The maize and rice U1-70K genes bear strong similarity to the Arabidopsis gene and each encode three transcripts in roots and shoots. Alternative splicing produces two transcripts from each gene in addition to the mRNA encoding the wild type protein. In both cases, selective inclusion of intron 6 or utilization of a cryptic donor site within intron 6 sequence generates the two alternatively spliced transcripts. This evolutionary conservation of splicing patterns between different plant species suggests an important biological function for alternative splicing in the expression of U1-70K gene.

Cross-species EST alignments reveal novel and conserved alternative splicing events in legumes

“…The sixth intron of U1-70K can be retained in At [ 22 ], an event conserved between At and Os [ 1 ]. Recently, the IntronR event was experimentally confirmed in Os and maize [ 23 ].…”

Dek42 encodes an RNA‐binding protein that affects alternative pre‐mRNA splicing and maize kernel development

“…DEK42 interacts with spliceosome components to participate in AS The interaction of spliceosome and splicing factors has been widely reported (Lopato et al 2002), especially between the components of U1/U2 subunits and the SR protein (Lopato et al 2002). U1-70K, an important component of U1 snRNP, is evolutionarily conserved between different plant species, such as Arabidopsis and maize (Gupta et al 2006). It has been reported to interact with at least five SR proteins, including SC35 (Barta et al 2008;Yan et al 2017), SCL33 (Golovkin and Reddy 1999;Yan et al 2017), SR45 (Day et al 2012), SRZ21 and SRZ22 (Golovkin and Reddy 1998), for binding to the 5 0 SS at the earliest stages in plants (Golovkin and Reddy 1999).…”

Heterogeneous Nuclear Ribonucleoprotein H1 Coordinates with Phytochrome and the U1 snRNP Complex to Regulate Alternative Splicing in Physcomitrella patens

“…Several U1 snRNP proteins, such as U1-70K, U1A, U1C, LUC7a/b, PRE-MRNA-PROCESSING FACTOR40a/b (PRP40a/b), LUC7-rl, PRP39-1 and PRP39-2, might be present in Arabidopsis (Arabidopsis thaliana), based on comparative sequence analysis and proteomics (Wang and Brendel, 2004;Koncz et al, 2012). The functions of several of these factors have been elucidated (Simpson et al, 1995;Golovkin and Reddy, 1996;Gupta et al, 2006;Wang et al, 2007). For example, PRP39, which was first identified in yeast (Saccharomyces cerevisiae), is required for the formation of a U1 snRNP complex as well as the splicing process (Lockhart and Rymond, 1994).…”