Alternative Splicing Modulation by a LAMMER Kinase Impinges on Developmental and Transcriptome Expression

Savaldi-Goldstein, Sigal; Aviv, D.; Davydov, Olga; Fluhr, Robert

doi:10.1105/tpc.011056

Cited by 71 publications

(58 citation statements)

References 63 publications

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“…tabacum cv Samsun was transformed as previously described (Horsch et al, 1985). N. tabacum cv BY2 cell suspension were transformed as described by Savaldi-Goldstein et al (2003).…”

Section: Plant and Cell Suspension Transformationmentioning

confidence: 99%

The Receptor for the Fungal Elicitor Ethylene-Inducing Xylanase Is a Member of a Resistance-Like Gene Family in Tomato

2004

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An ethylene-inducing xylanase (EIX) is a potent elicitor of plant defense responses in specific cultivars of tobacco (Nicotiana tabacum) and tomato (Lycopersicon esculentum). The LeEix locus in tomatoes was characterized by map-based cloning, which led to the identification of a novel gene cluster from which two members (LeEix1 and LeEix2) were isolated. Similar to the tomato Ve resistance genes in tomato plants, the deduced amino acid sequences encoded by LeEix1 and LeEix2 contain a Leu zipper, an extracellular Leu-rich repeat domain with glycosylation signals, a transmembrane domain, and a C-terminal domain with a mammalian endocytosis signal. Silencing expression of the LeEix genes prevented the binding of EIX to cells of an EIX-responsive plant and thus inhibited the hypersensitive response. Overexpression of either LeEix1 or LeEix2 genes in EIX-nonresponsive tobacco plants enabled the binding of EIX, although only LeEix2 could transmit the signal that induced the hypersensitive response. Overexpressing LeEix2 in mammalian COS-7 cells enables binding of EIX, indicating physical interaction between the EIX elicitor and LeEix2 gene product. Structural analysis of the LeEix proteins suggests that they belong to a class of cell-surface glycoproteins with a signal for receptor-mediated endocytosis. Mutating the endocytosis signal in LeEix2 (Tyr 993 to Ala) abolished its ability to induce the hypersensitive response, suggesting that endocytosis plays a key role in the signal transduction pathway

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“…tabacum cv Samsun was transformed as previously described (Horsch et al, 1985). N. tabacum cv BY2 cell suspension were transformed as described by Savaldi-Goldstein et al (2003).…”

Section: Plant and Cell Suspension Transformationmentioning

confidence: 99%

The Receptor for the Fungal Elicitor Ethylene-Inducing Xylanase Is a Member of a Resistance-Like Gene Family in Tomato

2004

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“…To examine the in vivo effects of PK12, a construct of the tobacco gene was introduced into Arabidopsis (which contains a homologous protein with 73% amino-acid identity) where it was shown to bind, phosphorylate and colocalize with the Arabidopsis splicing factor atSRp34/ SR1 (Savaldi-Goldstein et al, 2003). Lines expressing high levels of the transgene had high PK12 kinase activity and showed altered patterns of AS in three of the four splicing factor genes that were examined (Figure 4a), i.e.…”

Section: As and The Generation Of Plastic Organism-level Phenotypesmentioning

confidence: 99%

Quantitative and evolutionary biology of alternative splicing: how changing the mix of alternative transcripts affects phenotypic plasticity and reaction norms

Marden

2006

Heredity

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Alternative splicing (AS) of pre-messenger RNA is a common phenomenon that creates different transcripts from a single gene, and these alternative transcripts affect phenotypes. The majority of AS research has examined tissue and developmental specificity of expression of particular AS transcripts, how this specificity affects cell function, and how aberrant AS is related to disease. Few studies have examined quantitative between-individual variation in AS within a cell or tissue type, or in relation to phenotypes, but the results are compelling: quantitative variation in AS affects plastic traits such as stress, anxiety, fear, egg production, muscle performance, energetics and plant growth. Genomic analyses of AS are also at a nascent stage, but have revealed a number of significant evolutionary patterns. Growing knowledge of upstream genes and kinases that regulate AS provides the as-yet little explored potential to examine how these genes and pathways respond to environmental and genotype variables. Research in this area can provide glimpses of a labyrinth of genetic architectures that have rarely been considered in evolutionary and organismal biology, or in quantitative genetics. The scarcity of contribution to knowledge about AS from these fields is illustrated by the fact that heritability of quantitative variation in AS has not yet been determined for any gene in any organism. New research tactics that incorporate quantitative analyses of AS will allow organismal and evolutionary biologists to attain a fuller mechanistic understanding of many of the traits they study, and may lead to more rapid discovery of functionally important polymorphisms.

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“…Members of the LAMMER subfamily occur in a variety of organisms from yeast to human [5][6][7][8][9][10][11], showing a high degree of conservation in those domains that are essential for phosphotransfer and substrate recognition [12]. Furthermore, the ability of several LAMMER kinases to modulate splicing could indicate a common functional role [13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

“…In Drosophila, mutations in the LAMMER kinase Doa alter sexual differentiation and activity of the Doa locus is required for development of embryonic tissues [14,32]. It was recently shown that the pk12 LAMMER kinase also modulates development and gene expression in tobacco cells [16].…”

Section: Introductionmentioning

confidence: 99%

Protein kinase clk/STY is differentially regulated during erythroleukemia cell differentiation: a bias toward the skipped splice variant characterizes postcommitment stages

et al. 2005

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Clk/STY is a LAMMER protein kinase capable to phosphorylate serine/arginine-rich (SR) proteins that modulate premRNA splicing. Clk/STY alternative splicing generates transcripts encoding a full-length kinase and a truncated catalytically inactive protein. Here we showed that clk/STY, as well as other members of the family (e.g. clk2, clk3 and clk4), are up-regulated during HMBA-induced erythroleukemia cell differentiation. mRNAs coding for the full-length and the truncated forms were responsible for the overall increased expression. In clk/STY, however, a switch was observed for the ratio of the two alternative spliced products. In undifferentiated cells the full-length transcript was more abundant whereas the transcript encoding for the truncated form predominated at latter stages of differentiation. Surprisingly, overexpression of clk/STY did not alter the splicing switch upon differentiation in MEL cells. These results suggest that clk/STY might contribute to control erythroid differentiation by a mechanism that implicates a balance between these two isoforms.

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Alternative Splicing Modulation by a LAMMER Kinase Impinges on Developmental and Transcriptome Expression

Cited by 71 publications

References 63 publications

The Receptor for the Fungal Elicitor Ethylene-Inducing Xylanase Is a Member of a Resistance-Like Gene Family in Tomato

The Receptor for the Fungal Elicitor Ethylene-Inducing Xylanase Is a Member of a Resistance-Like Gene Family in Tomato

Quantitative and evolutionary biology of alternative splicing: how changing the mix of alternative transcripts affects phenotypic plasticity and reaction norms

Protein kinase clk/STY is differentially regulated during erythroleukemia cell differentiation: a bias toward the skipped splice variant characterizes postcommitment stages

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