Alternative splicing of basic chitinase gene<i>PR3b</i>in the low-nicotine mutants of<i>Nicotiana tabacum</i>L. cv. Burley 21

Ma, Haoran; Wang, Feng; Wang, Wenjing; Yin, Guoying; Zhang, Dingyu; Ding, Yongqiang; Timko, Michael P.

doi:10.1093/jxb/erw345

Cited by 8 publications

(4 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For instance, the Bjchitinase gene is induced by JA but only moderately by SA ( Rawat et al, 2017 ). Activity of the PR3b protein, a chitinase, is post-transcriptionally regulated by JA signaling ( Ma et al, 2016 ). Plant chitinase activity may be positively or negatively regulated by Ca 2+ ( Stressmann et al, 2004 ; Oliveira et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic and Metabolomic Analyses Reveal a Potential Mechanism to Improve Soybean Resistance to Anthracnose

Zhu

Yang

et al. 2022

Front. Plant Sci.

View full text Add to dashboard Cite

Anthracnose, caused by Colletotrichum truncatum, leads to large-scale reduction in quality and yield in soybean production. Limited information is available regarding the molecular mechanisms of resistance to anthracnose in soybean. We conducted a transcriptomic and targeted metabolomic analysis of pods from two soybean lines, “Zhechun No. 3” (ZC3) and ZC-2, in response to C. truncatum infection. Factors contributing to the enhanced resistance of ZC-2 to anthracnose compared with that of ZC3, included signal transduction (jasmonic acid, auxin, mitogen-activated protein kinase, and Ca2+ signaling), transcription factors (WRKY and bHLH), resistance genes (PTI1, RPP13, RGA2, RPS6, and ULP2B), pathogenesis-related genes (chitinase and lipid transfer protein), and terpenoid metabolism. Targeted metabolomic analysis revealed that terpenoid metabolism responded more promptly and more intensely to C. truncatum infection in ZC-2 than in ZC3. In vitro antifungal activity and resistance induction test confirmed that jasmonic acid, auxin signaling and terpenoids played important roles in soybean resistance to anthracnose. This research is the first study to explore the molecular mechanisms of soybean resistance to anthracnose. The findings are important for in-depth analysis of molecular resistance mechanisms, discovery of resistance genes, and to expedite the breeding of anthracnose-resistant soybean cultivars.

show abstract

Section: Discussionmentioning

confidence: 99%

Transcriptomic and Metabolomic Analyses Reveal a Potential Mechanism to Improve Soybean Resistance to Anthracnose

Zhu

Yang

et al. 2022

Front. Plant Sci.

View full text Add to dashboard Cite

show abstract

“…For instance, JA triggers the accumulation of BPEp , which encodes a bHLH transcription factor, via extensive AS at the posttranscriptional level, thus increasing cell expansion and petal size in Arabidopsis (Brioudeset al , 2009). Furthermore, JA regulates the splicing pattern ofPR3b , which is associated with tobacco resistance against fungal pathogens (Ma et al , 2016). Analogously, exogenous MeJA specifically induces AS of defense-related acidic chitinase and affects pathogen defense inCitrus clementina (Del Carratore et al , 2011).…”

Section: The Roles Of As For Ja-related Participants In Developmentmentioning

confidence: 99%

Emerging role of jasmonic acid in woody plant development

Zhu

Bao

Chen

et al. 2023

Preprint

View full text Add to dashboard Cite

Jasmonic acid (JA) is an important phytohormone in optimizing plant developmental growth and stress responses. The biosynthesis and signalling pathways of JA share common and unique features between herbaceous and woody plants. However, numerous reports have primarily focused on JA function in model plant species or herbaceous plants, leading to a notable lack of research on the JA regulatory mechanism in woody plants. In particular, the role of Jas in posttranscriptional regulation events such as alternative splicing (AS), has largely not been elucidated in trees. In this review article, we summarize the current progress of JA-involved regulation of developmental growth in different tree species. Along with an accumulating number of studies revealing that AS participates in the JA regulatory network in woody plants, we also update the multiple roles served by JA associated with similar properties and differential regulation within their herbaceous counterparts. The utilization of genetic studies and high-throughput proteogenomic approaches to analyse AS genes and splicing factors involved in JA signalling would further advance our understanding of JA modulation in woody plants.

show abstract

“…The nicotine concentration of tobacco leaves was measured by gas chromatography with minor modification [26]. Briefly, 0.1 g tobacco leaf sample was freeze-dried and ground into fine powder.…”

Section: Nicotine Concentrationmentioning

confidence: 99%

Moderate Boron Concentration Beneficial for Flue-Cured Tobacco (Nicotiana tabacum L.) Seedlings Growth and Development

Sun

et al. 2022

Agriculture

View full text Add to dashboard Cite

Boron (B) deficiency is a common phenomenon in most tobacco-planting areas in Yunnan, China. In 2020 and 2022, hydroponic experiments that contained B in a concentration gradient of 0.000, 0.125, 0.250, 0.750, 5.000, 10.000, 20.000, and 40.000 mmol L−1 were conducted to investigate tobacco cultivar K326′s agronomic traits, photosynthetic performance, antioxidant enzymes, and boron and nicotine concentration. As B concentration increased, indices including leaves biomass and net photosynthetic rate (Pn) generally increased first and then decreased, which was in contrast to antioxidant enzymes, including superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT). With increasing B concentration, boron content in tobacco seedlings increased significantly by 24.00~96.44%, while decreased nicotine content by 21.60~82.03%. The highest biomass and photosynthetic performance were obtained within 0.75 and 5.00 mmol L−1 treatments. The results of the sandy soil pot verification experiment were similar to the hydroponic experiment obtained. The beneficial mechanism of moderate B on tobacco seedlings is to maintain cell structure integrity, enhance photosynthetic capability, and promote root growth. Consequently, the optimum B concentration for tobacco seedlings is 0.75~5.00 mmol L−1, and applying 0.25~0.50 B kg hm−1 in soil under available B insufficiency could meet the needs of the growth of flue-cured tobacco.

show abstract

Alternative splicing of basic chitinase genePR3bin the low-nicotine mutants ofNicotiana tabacumL. cv. Burley 21

Abstract: HighlightAlternative splicing of the basic chitinase gene PR3b requires genes in the NIC loci of low-nicotine mutants of Burley 21 tobacco.

Cited by 8 publications

References 62 publications

Transcriptomic and Metabolomic Analyses Reveal a Potential Mechanism to Improve Soybean Resistance to Anthracnose

Transcriptomic and Metabolomic Analyses Reveal a Potential Mechanism to Improve Soybean Resistance to Anthracnose

Emerging role of jasmonic acid in woody plant development

Moderate Boron Concentration Beneficial for Flue-Cured Tobacco (Nicotiana tabacum L.) Seedlings Growth and Development

Contact Info

Product

Resources

About