Alternative splicing of human and mouse NPFF2 receptor genes: Implications to receptor expression

Abstract

Alternative splicing has an important role in the tissue-specific regulation of gene expression. Here we report that similar to the human NPFF2 receptor, the mouse NPFF2 receptor is alternatively spliced. In human the presence of three alternatively spliced receptor variants were verified, whereas two NPFF2 receptor variants were identified in mouse. The alternative splicing affected the 5 0 untranslated region of the mouse receptor and the variants in mouse were differently distributed. The mouse NPFF system may also have species-specific features since the NPFF2 receptor mRNA expression differs from that reported for rat.

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“…In rats, however, NPFFR2 was very strongly expressed in the HTH, substantia nigra, medulla, and spinal cord, moderately expressed in the AMY, choroid plexus, and retina, and weakly expressed in the thalamus (Figure 3) [101]. The central distribution of NPFFRs in mice was analyzed at cellular resolution via radioactive ISH [169] and in rats via RNAscope ISH [170]. The results slightly differed from those obtained with RT-PCR.…”

“…NPFFR2-producing cells appeared mostly in the lateral lemniscus and the principal and spinal trigeminal nuclei; moderate expression of NPFFR2 was seen in the zona incerta, HTH (subparaventricular region, POA, PVN, DMN), thalamus, NTS, and DMX in rats [170], while in mice, the olfactory bulb, ARC, thalamus, dorsal tegmentum, and NTS showed the strongest labeling (Figure 3) [169].…”