Alternative Surfaces for Microcarrier Culture of Animal Cells

Gebb, C; Clark, Julian M.; Hirtenstein, M. D.; Lindgren, G; Lundgren, B.; Lindskog, U.; Vretblad, Per

doi:10.1007/978-1-4615-9376-8_9

Cited by 12 publications

(12 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Since that time, a number of different materials including glass, polystyrene plastic, acrylamide, solid collagen, cellulose and liquid fluorocarbons have been successfully used as microcarriers (Giard, Thilly and Wang, 1977;Nielson and Johansson, 1980;Gebb, Clark and Hirtenstein, 1982;Obrenovitch, Maintier and Sene, 1982;Varani et al, 1983;Keese and Giaever, 1983). In addition, microcarriers have been produced which contain adhesion-supporting extracellular matrix components (or synthetic adhesionpromoting sequences) attached to the surface through covalent or non-covalent linkages (Varani et al, 1993(Varani et al, , 1995.…”

Section: Discussionmentioning

confidence: 99%

Attachment and growth of anchorage-dependent cells on a novel, charged-surface microcarrier under serum-free conditions

Varani

Piel

Josephs

et al. 1998

Current Applications of Cell Culture Engineering

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

Attachment and growth of anchorage-dependent cells on a novel, charged-surface microcarrier under serum-free conditions

Varani

Piel

Josephs

et al. 1998

Current Applications of Cell Culture Engineering

View full text Add to dashboard Cite

show abstract

“…Viruses and viral assays. Strains of poliovirus 1 (Mahoney LP), echovirus 7 (Wallace), echovirus 11 (Gregory), echovirus 12 (Travis), echovirus 14 (Tow), echovirus 27 (SEC), coxsackievirus B2 (Taylor), coxsackievirus B5 (Faulkner), coxsackievirus A9 (CME456), and coxsackievirus A16 (natural sewage isolate) were assayed by the plaque method of Dahling et al (8); reovirus 1 (Lang) was assayed by the method of Wallis et al (36); and simian rotavirus SA-11 was monitored by the procedure of Smith et al (33,34). In assaying for the rotavirus, each 100 ml of overlay medium was supplemented with 0.2 ml of a 1% DEAE-dextran solution and 2.0 ml of a stock pancreatin solution, prepared as previously described (8).…”

Section: Methodsmentioning

confidence: 99%

Optimization of the BGM cell line culture and viral assay procedures for monitoring viruses in the environment

Dahling¹,

Wright²

1986

Appl Environ Microbiol

View full text Add to dashboard Cite

An in-depth study of the continuous cell line designated BGM is described herein, and recommendations are made for standardizing cell culture and viral assay procedures. Based on data gathered from a survey of 58 laboratories using this cell line, a research plan was developed that included the study of growth media, sera, NaHCO3 levels, culture bottles, cell concentration, overlay media, agar, virus infection conditions, and cell-dissociating agents. Additionally, a comparative virus isolation study with BGM cells and nine other cell types was conducted with 37 sewage samples collected from nine different geographic areas. The results of the study indicated that the BGM cell line is superior for virus isolation when compared with the other cell types and that certain media and additives tend to increase BGM cell sensitivity to a specific group of viruses. A standardized procedure for cultivation of BGM cells is described which provides a more effective enterovirus assay system.

show abstract

“…Therefore, many researchers are attracted to this system for anchorage-dependent cells (11,12). Microcarrier systems have been widely used because of a large surface areato-volume ratio and a homogeneous environment (13)(14)(15)(16), although shear stress and slow initial cell attachment rate at high agitation speed may be a problem (17,18). Many anchorage-dependent cells can be adapted to growth as aggregate cultures in suspension.…”

Section: Introductionmentioning

confidence: 99%

Comparison of Different Bioreactor Systems for the Production of High Titer Retroviral Vectors

Merten

Cruz

Rochette

et al. 2001

Biotechnology Progress

View full text Add to dashboard Cite

Improved, human-based packaging cell lines allow the production of high-titer, RCR-free retroviral vectors. The utility of these cell lines for the production of clinical grade vectors critically depends on the definition of optimal conditions for scaled-up cultures. In this work, a clone derived from the TE Fly GALV packaging cell (Duisit et al. Hum. Gene Ther. 1999, 10, 189) that produces high titers of a lacZ containing retroviral vector with a Gibbon Ape Leukemia Virus envelope glycoprotein was used. This clone can produce (2-5) x 10(6) PFU cm(-3) in small scale cultures and has been evaluated for growth and vector production in different reactor systems. The performances of fixed bed reactors [CellCube (Costar) and Celligen (New Brunswick)] and stirred tank reactors [microcarriers and clump cultures] were compared. The cells showed a higher apparent growth rate in the fixed bed reactor systems than in the suspension systems, probably as a result of the fact that aggregation and/or formation of clumps led to a reduced viability and reduced growth of cells in the interior of the clumps. As a consequence, the final cell density and number were in average 3- to 7-fold higher in the fixed bed systems in comparison to the suspension culture systems. The average titers obtained ranged from 0.5 to 2.1 x 10(7) PFU cm(-3) for the fixed bed and microcarrier systems, while the clump cultures produced only (2-5) x 10(5) PFU cm(-3). The differences in titers reflect cell densities as well as specific viral vector production rates, with the immobilization and microcarrier systems exhibiting an at least 10-fold higher production rate in comparison to the clump cultures. A partial optimization of the culture conditions in the Celligen fixed bed reactor, consisting of a 9-fold reduction of the seeding cell density, led to a 5-fold increased vector production rate accompanied by an average titer of 3 x 10(7) PFU cm(-3) (maximum titer (4-5) x 10(7) PFU cm(-3)) in the fixed bed reactor. The performance evaluation results using mathematical models indicated that the fixed bed bioreactor has a higher potential for retroviral vector production because of both the higher reactor productivity and the lower sensitivity of productivity in relation to the changes in final retrovirus titer in the range of 3 x 10(6) to 15 x 10(6) PFU cm(-3).

show abstract

Alternative Surfaces for Microcarrier Culture of Animal Cells

Cited by 12 publications

References 14 publications

Attachment and growth of anchorage-dependent cells on a novel, charged-surface microcarrier under serum-free conditions

Attachment and growth of anchorage-dependent cells on a novel, charged-surface microcarrier under serum-free conditions

Optimization of the BGM cell line culture and viral assay procedures for monitoring viruses in the environment

Comparison of Different Bioreactor Systems for the Production of High Titer Retroviral Vectors

Contact Info

Product

Resources

About