Alternative use of phage display: phage M13 can remain viable in the intestines of poultry without causing damage

Santos, Francisco Abad; Valadares, Edson Campos; Goulart, Luiz Ricardo; Nunes, Pedro Lucas Figueiredo; Mendonça, Eliane Pereira; Girão, Lúcio Vilela Carneiro; Hora, Aline Santana da; Ferreira, Thatiana Bragine; Bastos, Luciana Machado; Medeiros-Ronchi, Alessandra Aparecida; Fonseca, Belchiolina Beatriz

doi:10.1186/s13568-022-01407-9

Cited by 3 publications

(1 citation statement)

References 12 publications

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“…Following publication of the original article (Santos et al 2022 ), the authors reported an error in the first author’s name “Fabiana de Almeida Araújo Santos”. The given name should be “Fabiana de Almeida Araújo” and last name is “Santos”.…”

Section: Correction To: Amb Express (2022) 12:64 Https://do...mentioning

confidence: 99%

Correction: Alternative use of phage display: phage M13 can remain viable in the intestines of poultry without causing damage

et al. 2022

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Section: Correction To: Amb Express (2022) 12:64 Https://do...mentioning

confidence: 99%

Correction: Alternative use of phage display: phage M13 can remain viable in the intestines of poultry without causing damage

et al. 2022

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A new standardization for the use of chicken embryo: selection of target from the phage display library and infection

de Souza,

Sommerfeld,

Almeida-Souza

et al. 2024

Appl Microbiol Biotechnol

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The filamentous bacteriophage M13KO7 (M13) is the most used in phage display (PD) technology and, like other phages, has been applied in several areas of medicine, agriculture, and in the food industry. One of the advantages is that they can modulate the immune response in the presence of pathogenic microorganisms, such as bacteria and viruses. This study evaluated the use of phage M13 in the chicken embryos model. We inoculated 13-day-old chicken embryos with Salmonella Pullorum (SP) and then evaluated survival for the presence of phage M13 or E. coli ER2738 (ECR) infected with M13. We found that the ECR bacterium inhibits SP multiplication in 0.32 (M13-infected ECR) or 0.44 log UFC/mL (M13-uninfected ECR) and that the ECR-free phage M13 from the PD library can be used in chicken embryo models. This work provides the use of the chicken embryo as a model to study systemic infection and can be employed as an analysis tool for various peptides that M13 can express from PD selection. Key points • SP-infected chicken embryo can be a helpful model of systemic infection for different tests. • Phage M13 does not lead to embryonic mortality or cause serious injury to embryos. • Phage M13 from the PD library can be used in chicken embryo model tests.

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A synthetic communication system uncovers extracellular immunity that self-limits bacteriophage transmission

Pathania

Hopper

Pandi

et al. 2022

Preprint

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Intercellular communication enables coordinated action by cells of microbial communities and multicellular organisms, often mediated by molecular exchange of information. Inspired by their success, synthetic biologists have recently started implementing population-level controls in engineered organisms with the aim of expanding circuit size and complexity. Yet, realising the true potential of multicellular synthetic biology requires an expanded communication alphabet as well as quantitative models to predict complex behaviour. Towards that aim, here we repurpose the M13 bacteriophage machinery for cell-to-cell communication between Escherichia coli cells and characterise the signalling dynamics. The fitted quantitative model includes the growth burden of the communication machinery, the relationship between cellular growth phase and the secretion-infection kinetics, and concurrent antibiotic selection. Limitations of deterministic models are demonstrated, with stochastic effects playing a key role in reproducing the observed infection kinetics. Surprisingly, we discover that the M13 minor coat protein pIII is released into the medium to confer extracellular immunity to uninfected cells. In a simulated gut environment, this mechanism enables the phage to farm uninfected bacterial cells for the future, increasing the overall success of both M13 and E. coli. In addition to establishing a tool for intercellular communication, our work uncovers the mutualistic nature of a phage-bacterial relationship that has evolved over long-term coexistence.

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Alternative use of phage display: phage M13 can remain viable in the intestines of poultry without causing damage

Cited by 3 publications

References 12 publications

Correction: Alternative use of phage display: phage M13 can remain viable in the intestines of poultry without causing damage

Correction: Alternative use of phage display: phage M13 can remain viable in the intestines of poultry without causing damage

A new standardization for the use of chicken embryo: selection of target from the phage display library and infection

A synthetic communication system uncovers extracellular immunity that self-limits bacteriophage transmission

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