DC bias generates a large DC component in the windings of the transformer in operation, thereby generating harmonics, causing vibration, noise, and overheating. In severe cases, it can cause damage to the transformer, which will affect the safe and stable operation of the AC system. This article takes the DC bias of the main transformer of a 220kV substation as an example, and uses the capacitance isolation method to suppress the DC bias. Due to the addition of capacitor equipment at the neutral point, the zero-sequence impedance of the main transformer and the change of the zero-sequence impedance of the power grid are changed, which has an impact on the zero-sequence (directional) current protection and ground distance protection. Through theoretical analysis and simulation research verification, for general 220kV substations, the capacitor DC isolation method (1.2Ω) can be used to suppress DC bias, which has a small impact on the relay protection and does not need to adjust the related protection settings.