Alternatives to conventional field evaluation for genetic selection for Fe-efficiency of soybean

Jessen, H. J.; Fehr, W. R.; Piper, T. E.

doi:10.1080/01904168609363449

Cited by 22 publications

(12 citation statements)

References 4 publications

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“…While genotype difference for percent callus weight reduction were observed at the each level, the 10 mM NaHCO 3 treatment provided the greatest differences. Previous studies using whole plants (9,15) have indicated that 15 mM NaHCO 3 was optimum for Fe-efficiency evaluation. However in the present study 15 mM of NaHCO 3 Compared to the control, callus weight of all ten cultivars was lower on the 10 mM NaHCO 3 .…”

Section: Resultsmentioning

confidence: 99%

“…Prior studies using bicarbonate containing nutrient solutions (5,9,15,10) have indicated that lower levels of iron (4-6 (xM) were required to differentiate between soybean genotypes for Fe-efficiency. The use of 100 uM Fe in the present investigation suggests the possibility of a differential response between soybean callus and soybean roots to iron.…”

Section: Resultsmentioning

confidence: 99%

“…Alternative screening techniques include nutrient solutions and tissue culture. Several researchers have developed nutrient solutions that can distinguish between Fe-efficient and Fe-inefficient genotypes (5,9,15). Despite the high correlations reported in these studies, nutrient solutions are rarely used due to the high expense associated with this technique and the limited number of genotypes that can be evaluated (15).…”

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confidence: 99%

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Soybean genotype evaluation for iron deficiency chlorosis using sodium bicarbonate and tissue culture

Graham¹,

Stephens²,

Widholm³

et al. 1992

Journal of Plant Nutrition

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Soybean genotype evaluation for iron deficiency chlorosis using sodium bicarbonate and tissue culture

Graham¹,

Stephens²,

Widholm³

et al. 1992

Journal of Plant Nutrition

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“…Field tests have, however, two important limitations: 1) evaluations can only be conducted during summer (one season per year), which limits genetic progress, and 2) there is the inability to artificially alter with the current technology, the severity of the test without imposing plant injury (Jessen et al, 1986). The nutrient solution technique in screening for chlorosis resistance used in some breeding programs was devised to overcome these limitations.…”

Section: Introductionmentioning

confidence: 99%

Nutrient solution screening of Fe chlorosis resistance in soybean evaluated by molecular characterization

Lin

Baumer²,

Ivers³

et al. 2000

Journal of Plant Nutrition

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Nutrient solution systems have been considered an alternative method to field evaluations for studies of iron deficiency chlorosis (IDC) and for breeding soybeans with improved iron efficiency. To map genes controlling IDC in nutrient solution, 120 F 24 lines in a Pride B216 × A15 population, and 92 F 24 lines in an Anoka × A7 population were grown in nutrient solution in greenhouse plantings and evaluated for IDC by visual scores and determinations of chlorophyll concentrations. Eighty-nine restriction fragment length polymorphism (RFLP) and 10 simple 1916 LINETAL.sequence repeat (SSR) markers in the Pride B216 x A15 population, and 82 RFLP, 14 SSR and one morphological (hilum color) markers in the Anoka x A7 population were used to construct linkage maps and to locate quantitative trait loci (QTL) controlling IDC. In the Anoka × A7 population, one major gene on linkage group N, and modifying QTL on linkage groups A1 and I previously mapped during field tests also were identified in the nutrient solution test. Different genetic linkage groups in soybean have been identified by letters, and as such will be used throughout the paper. One newly identified QTL was mapped on linkage group B2. In the Pride B216 × A15 population, one QTL previously mapped on linkage group I during field tests was not identified in the nutrient solution test, and two newly identified QTL were mapped on linkage groups A2 and B1. QTL on linkage groups B2, G, H, L, and N were identified in both field and nutrient solution tests. Due to significant interaction between genotype and environment in both field and nutrient solution tests, QTL identifications from multiple environments were used to compare the similarity between field and nutrient solution tests. We concluded that similar QTL are identified in nutrient solution and field tests and therefore, both systems identify similar genetic mechanisms of iron uptake and/or utilization.

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“…In azalea, putative-tolerant genotypes have been observed in Japanese wild habitats with alkaline soil (pH up to 8.0), but few data are available (Scariot and Kobayashi, 2008). While iron deficiency-tolerant genotypes are commonly selected by growing plants in calcareous soils, field tests often suffer from soil heterogeneity and variable environmental conditions (Jessen et al, 1986). To control plant growth conditions, one alternative is to use a homogeneous substrate capable of inducing iron chlorosis (Alcántara et al, 2012), such as hydroponics with sodium hydrogen carbonate (NaHCO3) as the medium buffer.…”

Section: Introductionmentioning

confidence: 99%

Hydroponic Screening for Iron Deficiency Tolerance in Evergreen Azaleas

Demasi

Caser

Kobayashi

et al. 2015

Not Bot Hort Agrobot Cluj

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Evergreen azaleas grow in acid soil and suffer from iron deficiency when cultivated in substrate with pH higher than 6.0. In order to select tolerant plants, 11 azalea genotypes were tested for 21 days in alkaline solution (pH 9), buffered with sodium hydrogen carbonate (1 g·l -1 ). Leaf damage, root length and mortality rate were recorded. While leaf damage and mortality rate allowed to discriminate genotypes, root development appeared not directly linked to iron deficiency tolerance. Rhododendron 'Juko', R. scabrum, R. macrosepalum 'Hanaguruma', R. x pulchrum 'Oomurasaki', and R. x pulchrum 'Sen-e-oomurasaki' resulted iron efficient genetic resources, useful for azalea cultivation and gardening in calcareous soils. On the contrary, R. obtusum 'Kirin', R. tosaense, R. x mucronatum 'Fujimanyo' and R. obtusum 'Susogo-no-ito' resulted iron deficiency sensitive genotypes. R. x mucronatum 'Ryukyushibori' and R. indicum 'Kinsai' showed intermediate responses.

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Alternatives to conventional field evaluation for genetic selection for Fe-efficiency of soybean

Cited by 22 publications

References 4 publications

Soybean genotype evaluation for iron deficiency chlorosis using sodium bicarbonate and tissue culture

Soybean genotype evaluation for iron deficiency chlorosis using sodium bicarbonate and tissue culture

Nutrient solution screening of Fe chlorosis resistance in soybean evaluated by molecular characterization

Hydroponic Screening for Iron Deficiency Tolerance in Evergreen Azaleas

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