Alternatives to Trichoderma reesei in biofuel production

Gusakov, Alexander V.

doi:10.1016/j.tibtech.2011.04.004

Cited by 344 publications

(233 citation statements)

References 43 publications

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“…Subsequently, a secondary fungal species, such as an industrial yeast strain, can ferment any liberated monomeric sugars into ethanol. Various candidates for the primary fungus are known, which secrete the required arsenal of lignocellulolytic enzymes, including Aspergillus niger, Aspergillus oryzae, Trichoderma reesei and Humicola insolens [8]. The production of the Japanese alcoholic beverage sake utilises a consortium of A. oryzae and S. cerevisiae (NCYC479) to produce high concentrations of ethanol (ca.…”

Section: Introductionmentioning

confidence: 99%

Bioethanol Production from Brewers Spent Grains Using a Fungal Consolidated Bioprocessing (CBP) Approach

et al. 2016

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Production of bioethanol from brewers spent grains (BSG) using consolidated bioprocessing (CBP) is reported. Each CBP system consists of a primary filamentous fungal species, which secretes the enzymes required to deconstruct biomass, paired with a secondary yeast species to ferment liberated sugars to ethanol. Interestingly, although several pairings of fungi were investigated, the sake fermentation system (A. oryzae and S. cerevisiae NCYC479) was found to yield the highest concentrations of ethanol (37 g/L of ethanol within 10 days). On this basis, 1 t of BSG (dry weight) would yield 94 kg of ethanol using 36 hL of water in the process. QRT-PCR analysis of selected carbohydrate degrading (CAZy) genes expressed by A. oryzae in the BSG sake system showed that hemicellulose was deconstructed first, followed by cellulose. One drawback of the CBP approach is lower ethanol productivity rates; however, it requires low energy and water inputs, and hence is worthy of further investigation and optimisation.

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Section: Introductionmentioning

confidence: 99%

Bioethanol Production from Brewers Spent Grains Using a Fungal Consolidated Bioprocessing (CBP) Approach

et al. 2016

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“…CTec2 is based on genetically modified strains of T. reesei 17) and has high β-glucosidase activity 43) and xylanase activity.…”

Section: Discussionmentioning

confidence: 99%

Newly isolated Penicillium oxalicum A592-4B secretes enzymes that degrade milled rice straw with high efficiency

Aoyama

Kurane

Matsuura

et al. 2015

Bioscience, Biotechnology, and Biochemistry

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An enzyme producing micro-organism, which can directly saccharify rice straw that has only been crushed without undergoing the current acid or alkaline pretreatment, was found. From the homology with the ITS, 28S rDNA sequence, the strain named A592-4B was identified as Penicillium oxalicum. Activities of the A592-4B enzymes and commercial enzyme preparations were compared by Novozymes Cellic CTec2 and Genencore GC220. In the present experimental condition, activity of A592-4B enzymes was 2.6 times higher than that of CTec2 for degrading milled rice straw. Furthermore, even when a quarter amount of A592-4B enzyme was applied to the rice straw, the conversion rate was still higher than that by CTec2. By utilizing A592-4B enzymes, improved lignocellulose degradation yields can be achieved without pre-treatment of the substrates; thus, contributing to cost reduction as well as reducing environmental burden.

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“…The cellulolytic enzymatic complex is produced by a wide variety of microorganisms (bacteria and fungi); however, the aerobic fungi are known for their high growth and protein secretion rates (Lynd et al, 2002). Most commercial cellulases are produced by filamentous fungi of the genera Trichoderma and Aspergillus (Bhat, 2000;Gusakov, 2011). Among the Aspergillus genera, A. niger along with A. oryzae are the two most important fungi worldwide for biotechnological applications (Hu et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

BIOPROCESS DEVELOPMENTS FOR CELLULASE PRODUCTION BY Aspergillus oryzae CULTIVATED UNDER SOLID-STATE FERMENTATION

Pirota

Tonelotto

Delabona

et al. 2016

Braz. J. Chem. Eng.

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-Bioprocess development studies concerning the production of cellulases are of crucial importance due to the significant impact of these enzymes on the economics of biomass conversion into fuels and chemicals. This work evaluates the effects of solid-state fermentation (SSF) operational conditions on cellulase production by a novel strain of Aspergillus oryzae using an instrumented lab-scale bioreactor equipped with an on-line automated monitoring and control system. The use of SSF cultivation under controlled conditions substantially improved cellulase production. Highest production of FPase (0.40 IU g -1 ), endoglucanase (123.64 IU g -1 ), and -glucosidase (18.32 IU g -1 ) was achieved at 28 C, using an initial substrate moisture content of 70%, with an inlet air humidity of 80% and an airflow rate of 20 mL min -1 . Further studies of kinetic profiles and respirometric analyses were performed. The results showed that these data could be very useful for bioprocess development of cellulase production and scale-up.

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Alternatives to Trichoderma reesei in biofuel production

Cited by 344 publications

References 43 publications

Bioethanol Production from Brewers Spent Grains Using a Fungal Consolidated Bioprocessing (CBP) Approach

Bioethanol Production from Brewers Spent Grains Using a Fungal Consolidated Bioprocessing (CBP) Approach

Newly isolated Penicillium oxalicum A592-4B secretes enzymes that degrade milled rice straw with high efficiency

BIOPROCESS DEVELOPMENTS FOR CELLULASE PRODUCTION BY Aspergillus oryzae CULTIVATED UNDER SOLID-STATE FERMENTATION

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