Alumina nanoparticles size and crystalline phase impact on cytotoxic effect on alveolar epithelial cells after simple or HCl combined exposures

Bourgois, Alexandra; Crouzier, David; Legrand, François-Xavier; Florent, R; Boyard, Alexandre; Gîrleanu, Maria; Favier, Anne-Laure; François, Sabine; Dekali, Samir

doi:10.1016/j.tiv.2019.04.016

Cited by 4 publications

(3 citation statements)

References 67 publications

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“…Therefore Al 2 O 3 NPs crystallinity is an important physico-chemical parameter to characterize, as it was shown that these NPs could induce or not fibrotic effects depending on their crystallinities [ 29 ]. This was also demonstrated in several human cell lines, including bronchial or alveolar epithelial cells, that cytotoxicity could be modulated depending on the crystallinities of Al 2 O 3 particles [ 34 , 39 ]. On the other hand, Bourgois et al exposed rats by nose-only inhalation to a strong concentration of γ/δ-Al 2 O 3 NPs, also showing increased inflammatory response after five days [ 14 ].…”

Section: Discussionmentioning

confidence: 98%

“…Authors hypothesized that biological effects after exposure to particles would appear to depend on chemical composition as well as size, shape, and cell type. Recently, Bourgois et al also exposed 24 h A549 alveolar epithelial cells to Al 2 O 3 particles [ 39 ]. They did not show any effects of different particle sizes and crystallinities on normalized cell index, cell viability, reduced glutathione, and double DNA strand breaks.…”

Section: In Vitro Studies Of Cytotoxic Mechanisms Induced By Alumina ...mentioning

confidence: 99%

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Critical Review on Toxicological Mechanisms Triggered by Inhalation of Alumina Nanoparticles on to the Lungs

Dekali¹,

Bourgois

François

2022

Biomedicines

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Alumina nanoparticles (Al2O3 NPs) can be released in occupational environments in different contexts such as industry, defense, and aerospace. Workers can be exposed by inhalation to these NPs, for instance, through welding fumes or aerosolized propellant combustion residues. Several clinical and epidemiological studies have reported that inhalation of Al2O3 NPs could trigger aluminosis, inflammation in the lung parenchyma, respiratory symptoms such as cough or shortness of breath, and probably long-term pulmonary fibrosis. The present review is a critical update of the current knowledge on underlying toxicological, molecular, and cellular mechanisms induced by exposure to Al2O3 NPs in the lungs. A major part of animal studies also points out inflammatory cells and secreted biomarkers in broncho-alveolar lavage fluid (BALF) and blood serum, while in vitro studies on lung cells indicate contradictory results regarding the toxicity of these NPs.

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Section: Discussionmentioning

confidence: 98%

Section: In Vitro Studies Of Cytotoxic Mechanisms Induced By Alumina ...mentioning

confidence: 99%

Critical Review on Toxicological Mechanisms Triggered by Inhalation of Alumina Nanoparticles on to the Lungs

Dekali¹,

Bourgois

François

2022

Biomedicines

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“…Control cells were only incubated with LSGS-supplemented Medium 200 (Gibco). Results are expressed using normalized cell index (NCI) as described by Bourgois et al (2019) .…”

Section: Methodsmentioning

confidence: 99%

Effects of preconditioning with TNFα and IFNγ in angiogenic potential of mesenchymal stromal cell-derived extracellular vesicles

Cavallero,

Dekali,

Guitard

et al. 2023

Front. Cell Dev. Biol.

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Introduction: Mesenchymal stromal cells (MSCs) have demonstrated therapeutic properties both in vitro and in vivo to treat various diseases, including anti-inflammatory, immunomodulatory and pro-angiogenic effects. These therapeutic effects are mediated by their secretome composed of soluble factors and extracellular vesicles (EVs). The composition of EVs reflects the molecular and functional characteristics of parental cells. MSC preconditioning can alter the composition of EVs, thereby influencing their therapeutic potential.Methods: MSCs were subjected to preconditioning with two cytokines, TNFα and IFNγ. Following 24 h of preconditioning, MSC-EVs secreted into the culture supernatant were isolated through tangential filtration. Particle concentration and size distribution were measured by nanoparticle tracking analysis, and the surface antigen expression of the EV-specific CD63 was quantified via Enzyme Linked ImmunoSorbent Assay. The angiogenic potential of MSCEVs obtained after preconditioning MSCs was assessed by the analysis of their protein composition and their influence on human umbilical vein endothelial cell (HUVECs) proliferation, migration, and tube-forming ability.Results: Preconditioning with TNFα and IFNγ did not influence the MSC-EV profile but did induce changes in their protein content. Indeed, the expression of pro-angiogenic proteins increased in EVs from preconditioned MSCs compared to EVs from no-preconditioned MSCs. EVs from preconditioned MSCs tend to stimulate HUVEC migration, proliferation and tubeforming ability. These observations imply the presence of a pro-angiogenic potential in EVs obtained after preconditioning of MSCs with TNFα and IFNγ.Discussion: In conclusion, it appears that the pro-angiogenic potential of EVs is enhanced through preconditioning of MSCs with TNFα and IFNγ. The use of these MSCs-EVs in therapy would circumvent the limitations of current cell-based therapies. Indeed, the therapeutic potential of MSC-EVs presents an attractive strategy for exploiting the clinical benefits of MSC therapy. For example, in the field of regenerative medicine, the exploitation of cell-free therapy using highly pro-angiogenic MSC-EVs is of great interest.

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Anti-corrosion and cytotoxicity properties of inorganic surface treatments on Mg1Ca biodegradable alloy

Neves,

Sousa,

Freitas

et al. 2024

Surface and Coatings Technology

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Alumina nanoparticles size and crystalline phase impact on cytotoxic effect on alveolar epithelial cells after simple or HCl combined exposures

Cited by 4 publications

References 67 publications

Critical Review on Toxicological Mechanisms Triggered by Inhalation of Alumina Nanoparticles on to the Lungs

Critical Review on Toxicological Mechanisms Triggered by Inhalation of Alumina Nanoparticles on to the Lungs

Effects of preconditioning with TNFα and IFNγ in angiogenic potential of mesenchymal stromal cell-derived extracellular vesicles

Anti-corrosion and cytotoxicity properties of inorganic surface treatments on Mg1Ca biodegradable alloy

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