Aluminum effects on embryo suspensor polytene chromosomes of<i>Phaseolus coccineus</i>L

Bartoli, Gianni; Toppi, Luigi Sanità di; Andreucci, Andrea; Castiglione, Monica Ruffini

doi:10.1080/11263504.2017.1359213

Cited by 3 publications

(1 citation statement)

References 42 publications

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“…While primary physiological response is at the root level Al toxicity results in strong yield reduction (Gupta et al, 2013). Poor root growth, changed root morphology, stunted plant growth, thicker lateral and taproots, browning of roots, inefficient water and nutrient uptake, and accumulation of Al and other toxic ions (Mugai et al, 2000;Bartoli et al, 2017), are symptoms of Al toxicity induced in susceptible lines of common bean (Rao et al, 2008(Rao et al, , 2016.…”

Section: Introductionmentioning

confidence: 99%

Genome Wide Association Mapping of Root Traits in the Andean Genepool of Common Bean (Phaseolus vulgaris L.) Grown With and Without Aluminum Toxicity

Ambachew

Blair

2021

Front. Plant Sci.

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Common bean is one of the most important grain legumes for human diets but is produced on marginal lands with unfavorable soil conditions; among which Aluminum (Al) toxicity is a serious and widespread problem. Under low pH, stable forms of Al dissolve into the soil solution and as phytotoxic ions inhibit the growth and function of roots through injury to the root apex. This results in a smaller root system that detrimentally effects yield. The goal of this study was to evaluate 227 genotypes from an Andean diversity panel (ADP) of common bean and determine the level of Al toxicity tolerance and candidate genes for this abiotic stress tolerance through root trait analysis and marker association studies. Plants were grown as seedlings in hydroponic tanks at a pH of 4.5 with a treatment of high Al concentration (50 μM) compared to a control (0 μM). The roots were harvested and scanned to determine average root diameter, root volume, root surface area, number of root links, number of root tips, and total root length. Percent reduction or increase was calculated for each trait by comparing treatments. Genome wide association study (GWAS) was conducted by testing phenotypic data against single nucleotide polymorphism (SNP) marker genotyping data for the panel. Principal components and a kinship matrix were included in the mixed linear model to correct for population structure. Analyses of variance indicated the presence of significant difference between genotypes. The heritability of traits ranged from 0.67 to 0.92 in Al-treated and reached similar values in non-treated plants. GWAS revealed significant associations between root traits and genetic markers on chromosomes Pv01, Pv04, Pv05, Pv06, and Pv11 with some SNPs contributing to more than one trait. Candidate genes near these loci were analyzed to explain the detected association and included an Al activated malate transporter gene and a multidrug and toxic compound extrusion gene. This study showed that polygenic inheritance was critical to aluminum toxicity tolerance in common beans roots. Candidate genes found suggested that exudation of malate and citrate as organic acids would be important for Al tolerance. Possible cross-talk between mechanisms of aluminum tolerance and resistance to other abiotic stresses are discussed.

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Section: Introductionmentioning

confidence: 99%

Genome Wide Association Mapping of Root Traits in the Andean Genepool of Common Bean (Phaseolus vulgaris L.) Grown With and Without Aluminum Toxicity

Ambachew

Blair

2021

Front. Plant Sci.

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Evaluation and selection of alfalfa genotypes for tolerance to aluminium toxic stress

Liatukienė,

Skuodienė,

Norkevičienė

et al. 2024

Front. Plant Sci.

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Alfalfa is one of the most important and the most cultivated crop due to its high nutritive quality and yield, but adaptation of alfalfa genotypes differ in terms of mobile aluminium stress in the soil. The aim of this study was to evaluate the tolerance to mobile Al concentrations in the laboratory and in the naturally acidic soil and select the promising genotypes based on agro-biological traits. In 2019, a laboratory experiment was conducted at the Institute of Agriculture of LAMMC. The experiment in the acidic soil with different mobile Al concentrations was conducted at the Vėžaičiai Branch of LAMMC. In 2020, the crops of alfalfa genotypes (11 cultivars and 3 populations) were established on Balthygleyic Dystric Retisol. The agro-biological traits were assessed during the 2021–2022 season. The tolerance index of hypocotyls and roots was evaluated using the filter-based screening method at different AlCl3 (0.0–64 mM) concentrations. The study results of the filter-based screening method showed that the genotype Žydrūnė, Malvina, Jõgeva 118, Skriveru, and 3130 were the most tolerant ones and the hypocotyl tolerance index of these genotypes was higher compared to medium tolerant genotypes Birutė, PGR12489, Europe and AJ2024 at 8, 16, 32 and 64 mM AlCl3 concentrations. The hypocotyl and root tolerance index of medium tolerant genotypes was higher compared to a sensitive genotype PGR10249 at 8 and 16 mM AlCl3. The study of cluster analysis with mobile Al 0.0–65.0 mg kg-1 showed that the genotypes Žydrūnė, Europe, AJ2024 and 3130 were the best in terms of wintering and spring regrowth, the cultivar Malvina had the best value of wintering, height before flowering and stem number, the cultivar Birutė had the best value of spring regrowth, height before flowering and seed yield, and the cultivar Skriveru had the best value of spring regrowth, height before flowering, stem number and seed yield.

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Assessing Aluminum Stress Resilience in Common Bean Roots: Phenotypic, Histochemical, and PvGST/PvPOD Gene Expression Analysis

Yeken

2024

Black Sea Journal of Agriculture

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Common bean (Phaseolus vulgaris L.) is grown in various parts of the world. Aluminum (Al) toxicity poses a significant and widespread challenge in marginal areas with unfavorable soil qualities where common bean is grown. In acidic soils, stable forms of Al dissolve into the soil solution and inhibit root growth and function by injuring the root apex with phytotoxic ions. This leads to the development of a smaller root system, adversely affecting crop yield. The aim of this study was to investigate the phenotypic evaluation for relative root elongation of 10 common bean genotypes/cultivars under Al stress (50 µM) and control (0 µM) conditions, the impact of Al toxicity using different histochemical dyes (Evan’s blue and Schiff’s reagent) and the expression levels of PvGST (Glutathione S-transferases) and PvPOD (peroxidase) genes in the root tissues of the most resistant/sensitive common beans under Al stress (50 µM) and control conditions (0 µM). The maximum relative root elongation value (71.9%) was found in Önceler-98 cultivar, while the lowest value was obtained from Blksr-19 (14.1%). Histochemical applications used in the study supported phenotypic results. The cracks at the root tip and high blue color intensity in the Blksr-19 genotype were detected in Schiff’s reagent and Evan’s blue dyes, respectively. The expression levels of PvGST and PvPOD genes in the root tissue of the Blksr-19 genotype (Al-sensitive) were highly upregulated at 24 h of Al stress treatment. The results revealed for the first time that these genes might be involved in the common bean root tissue's defense mechanism against Al stress for the first time. The findings herein will help plant breeders develop common bean cultivars tolerant to Al toxicity.

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Aluminum effects on embryo suspensor polytene chromosomes ofPhaseolus coccineusL

Cited by 3 publications

References 42 publications

Genome Wide Association Mapping of Root Traits in the Andean Genepool of Common Bean (Phaseolus vulgaris L.) Grown With and Without Aluminum Toxicity

Genome Wide Association Mapping of Root Traits in the Andean Genepool of Common Bean (Phaseolus vulgaris L.) Grown With and Without Aluminum Toxicity

Evaluation and selection of alfalfa genotypes for tolerance to aluminium toxic stress

Assessing Aluminum Stress Resilience in Common Bean Roots: Phenotypic, Histochemical, and PvGST/PvPOD Gene Expression Analysis

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