Aluminum ion mediated stabilization of silica-based anion-exchange packings to caustic regenerants

Pfannkoch, Edward A.; Switzer, Barbara S.; Kopaciewicz, W.

doi:10.1016/s0021-9673(01)81518-1

Cited by 4 publications

(3 citation statements)

References 8 publications

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“…For instance, diol-bonded silica has frequently been used in affinity separations with application and elution buffers in the range of pH 2.5-7.5 [28]. Further improvements in this stability might be obtained by using silica that incorporates zirconium or aluminum into its surface for work under more alkaline conditions [29,30] or by using various synthetic approaches for enhancing the low pH stability of silicabased bonded phases [23,31]. In this last area, the best stability at low pH values is achieved by using sterically protected silane stationary phases [23,27,31].…”

Section: Advantages and Limitations Of Silica In Affinity Separationsmentioning

confidence: 99%

Applications of silica supports in affinity chromatography

Schiel

Mallik

Soman

et al. 2006

J of Separation Science

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The combined use of silica-based chromatographic supports with immobilized affinity ligands can be used in many preparative and analytical applications. One example is the use of silica-based affinity columns in HPLC, giving rise to a method known as high-performance affinity chromatography (HPAC). This review discusses the role that silica has played in the development of affinity chromatography and HPAC and the applications of silica in these methods. This includes a discussion of the types of ligands that have been employed with silica and the methods by which these ligands have been immobilized. Various formats have also been presented for the use of silica in affinity chromatographic methods, including assays involving direct or indirect analyte detection, on-line or off-line affinity extraction, and chiral separations. The use of silica-based affinity columns in studies of biological systems based on zonal elution and frontal analysis methods will also be considered.

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Section: Advantages and Limitations Of Silica In Affinity Separationsmentioning

confidence: 99%

Applications of silica supports in affinity chromatography

Schiel

Mallik

Soman

et al. 2006

J of Separation Science

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show abstract

“…The latter constitutes a functional group that could be easily modified for ligand coupling and increases the stability of the silica support in elution buffers in the pH range from 2.5 up to 7.5 . Further improvements in the silica stability may be obtained by using a silica surface containing zirconium or alumina to work under more alkaline conditions. , …”

Section: Introductionmentioning

confidence: 99%

“…3 Further improvements in the silica stability may be obtained by using a silica surface containing zirconium or alumina to work under more alkaline conditions. 4,5 The combination of a high pressure liquid chromatography (HPLC) involving a silica support with an affinity ligand is now referred to as high performance affinity chromatography (HPAC). However, a limitation in using these supports for affinity chromatography concerns the pore size of the materials.…”

Section: Introductionmentioning

confidence: 99%

Optimization of the Properties of Macroporous Chromatography Silica Supports through Surface Roughness Control

et al. 2009

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Silica supports could constitute interesting stationary phases for the separation of biological substrates by affinity chromatography because of their better mechanical strength compared to polymers. Pore sizes and surface areas of the supports should be large enough (>70 nm) to provide high binding capacity and high mass transfer. However, most commercially available large-pore silicas usually exhibit very low surface areas. In the present work, we show how to increase the surface area of macroporous (80 nm) low surface area (16 m 2 /g) silica particles by a pseudomorphic transformation process occurring only at the surface of the pores, while preserving the macropores and the particle sizes of the parent silica. The transformation occurs through a controlled dissolution/reprecipitation mechanism in the presence of longchain alkylammonium surfactants. Consequently, the surface area of these macroporous silica materials was increased by a factor of 10 to 20 and was tuned between 100 and 300 m 2 /g. Moreover, this higher surface area allows the amount of functional groups immobilized at the surface of the materials to be increased by covalent grafting. The anchorage of glycidoxy groups at the surface of these new silicas produce materials with a high binding capacity for further protein coupling, comparable to polymeric phases. These new silica phases feature a high mechanical stability and represent an attractive alternative to polymeric phases in the area of large molecule separations.

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Advanced sorbents for preparative protein separation purposes

Boschetti¹

1994

Journal of Chromatography A

180

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Aluminum ion mediated stabilization of silica-based anion-exchange packings to caustic regenerants

Cited by 4 publications

References 8 publications

Applications of silica supports in affinity chromatography

Applications of silica supports in affinity chromatography

Optimization of the Properties of Macroporous Chromatography Silica Supports through Surface Roughness Control

Advanced sorbents for preparative protein separation purposes

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