Alveolar gene expression of tight junction protein in nicotine rats treated with zinc and vitamin D

Abstract

The current study aimed to investigation the role of zinc and vitamin D in modulating alveolar response of nicotine stress as a model of mammals male Wistar rats. Thirty mature males werekept at 23 ± 2 C°, randomly assigned to five equal groups and treatment for 14 days, C = Control drenched vehicle without treatment, G1 = injected with i/p nicotine 1.5 mg/ kg b.w., G2 = administrated orally of zinc 60 mg/ kgb.w., G3 = administrated orally of vitamin D 250 µg/ kg b.w., G4 = administrated orally both of zinc and vitamin D with same doses stressed and nicotine 1.5 mg/ kgb.w.i/p. Rats were anesthetized with ketamine at the end of the treatment period with xylazine and blood samples have been collected from the optical vein for estimation of serum ferritin and transferrin, then animals were sacrificed and lung was removed and weighted.All groups of rats had lung samples extracted rapidly, dipped in DEPC solution, and frozen under liquid nitrogen for determination of tight junction protein ( TJP) gene expression by RT-PCR analyses. Nicotine-stressed rats treated with zinc and vitamin D (G4) havesignificant highly increased expression of alveolar tight junction protein when compared with nicotine group (G1).

Effectiveness of Sodium Thiosulfate and Resveratrol in Remodeling Lung Injury and Expression of BCL2 in Nicotine-Stressed Rats

“…The depletion of antioxidants takes place, leading to dysfunction and harm at the cellular level [10]. The occurrence of oxidative stress in the lungs as a result of nicotine exposure has been documented in many rat models Cell membrane damage is a consequence of lipid peroxidation resulting from heightened oxidative stress, [11] [12]. According to the research conducted by [13] [14], it was found that the assessment of total antioxidant capacity and malondialdehyde (MDA) levels is widely regarded as a reliable and robust method for detecting the presence of stressful conditions.…”