Amaranth peptides with antithrombotic activity released by simulated gastrointestinal digestion

Sabbione, Ana Clara; Nardo, Agustina Estefanía; Añón, Marı́a Cristina; Scilingo, Adriana Alicia

doi:10.1016/j.jff.2015.10.015

Cited by 56 publications

(28 citation statements)

References 49 publications

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“…Additionally, the absorption of the peptide fraction was analysed with an in vitro assay of peptide transport through intestinal epithelium. The authors observed that some peptides were able to cross the Caco2‐TC7 cell monolayer (Sabbione et al ., ). Antiplatelet peptides released by simulated gastrointestinal digestion of oat (IC 50 = 0.282 mg mL −1 ), barley (IC 50 = 0.290 mg mL −1 ) and buckwheat (IC 50 = 0.328 mg mL −1 ) flours have been reported by Yu et al .…”

Section: Introductionmentioning

confidence: 97%

“…In the study conducted by Sabbione et al . (), an amaranth protein isolate was obtained and subjected to simulated gastrointestinal digestion to evaluate its potential antithrombotic activity. The peptide fraction obtained from the amaranth protein hydrolysate exhibited higher antithrombotic activity (IC 50 = 0.07 ± 0.01 mg mL −1 ) (IC 50 – a sample concentration providing 50% inhibition) than protein hydrolysate (IC 50 = 0.23 ± 0.02 mg mL −1 ).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Digestion and bioavailability of bioactive phytochemicals

Karaś

Jakubczyk

Szymanowska

et al. 2016

Int J of Food Sci Tech

136

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There are many scientific reports on determination of the content and biological activity of compounds found in food. However, these analyses are not sufficient to determine their effect on the human body. During digestion of food ingredients, many changes can modify their structure and this may affect their absorption and bioactivity. Many phenolic aglycones are hydrophilic and can be absorbed through biological membranes by diffusion. However, most polyphenols occur in the glycosidic form, which undoubtedly affects their absorption in the intestine. Oligopeptides are also absorbable via secondary active transport but based on the hydrogen ion gradient or with transporter PepT1. The bioavailability of phytochemicals is determined by their molecular weight or chemical structure and the food matrix. Accordingly, the aim of this work was to present the novel scientific reports related to the influence the many factors on digestibility, bioaccessibility and activity of selected bioactive compounds of plant origin.

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Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Digestion and bioavailability of bioactive phytochemicals

Karaś

Jakubczyk

Szymanowska

et al. 2016

Int J of Food Sci Tech

136

View full text Add to dashboard Cite

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“…Calculations were made according to Sabbione et al . (). Digestion was performed in two independent trials per sample.…”

Section: Methodsmentioning

confidence: 97%

“…In particular, amaranth peptides have exerted various biological activities (Silva‐Sánchez et al ., ). In vitro and in vivo antithrombotic and antihypertensive activity of amaranth peptides has been previously described in our laboratory (Vecchi & Añón, ; Fritz et al ., ; Sabbione et al ., , ).…”

Section: Introductionmentioning

confidence: 99%

Amaranth functional cookies exert potential antithrombotic and antihypertensive activities

Sabbione

Suarez

Añón

et al. 2018

Int J of Food Sci Tech

Self Cite

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In this study, amaranth flour was used as an ingredient to prepare gluten-free cookies. The production process and attributes of amaranth cookies were characterised, and the potential use of amaranth flour as a functional ingredient was analysed. Cookies exhibited a non-uniform reddish brown colour and a cookie factor ratio of 4.5 AE 0.6. Storage studies indicated that after 3 weeks at room temperature cookies presented slight variations in the texture. Simulated gastrointestinal digestion of this product was able to release peptides capable of exerting potential antithrombotic and antihypertensive activities, IC 50 values of 0.22 AE 0.04 and 0.23 AE 0.03 mg mL À1 protein, respectively. This work demonstrates for the first time that food made with amaranth flour exerts potential antithrombotic and antihypertensive activity. In conclusion, these amaranth cookies could be an alternative way of incorporating potentially health beneficial products for people who choose a conscious diet, including coeliac or vegan consumers.

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“…Guo and others () established the anti‐fatigue activity of peptides obtained after papain hydrolysis of sea horse protein extract. Sabbione and others () showed the antithrombotic activity of amaranth protein produced after imitation of gastrointestinal digestion, and they also detected that various peptides are capable to cross the Caco2‐TC7 cell monolayer. Alamdari and Ehsani () underlined the antimicrobial peptides obtained by hydrolysis of milk proteins using digestive proteases or by lactic acid bacteria fermentation.…”

Section: Protease Applicationsmentioning

confidence: 97%

Biotechnological Applications of Proteases in Food Technology

Tavano

Berenguer-Murcia

Secundo

et al. 2018

Comp Rev Food Sci Food Safe

236

105

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This review presents some of the hottest topics in biotechnological applications: proteases in biocatalysis. Obviously, one of the most relevant areas of application is in the hydrolysis of proteins in food technology, and that has led to a massive use on proteomics. The aim is to identify via peptide maps the different proteins obtained after a specific protease hydrolysis. However, concepts like degradomics are also taking on a more relevant importance in the use and study of proteases and will also be discussed. Other protease applications, as seem in cleaning (detergent development), the pharmaceutical industry, and in fine chemistry, will be analyzed. This review progresses from basic areas such as protease classification to a discussion of the preparation of protease-immobilized biocatalysts, considering the different problems raised by the use of immobilized proteases due to the peculiar features of the substrates, usually large macromolecules. Production of bioactive peptides via limited hydrolysis of proteins will occupy an important place in this review.

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Amaranth peptides with antithrombotic activity released by simulated gastrointestinal digestion

Cited by 56 publications

References 49 publications

Digestion and bioavailability of bioactive phytochemicals

Digestion and bioavailability of bioactive phytochemicals

Amaranth functional cookies exert potential antithrombotic and antihypertensive activities

Biotechnological Applications of Proteases in Food Technology

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