Ambiguity of the effect of high and low doses of amino-acid preparations on the immune response and phagocytosis in mice

Abstract

It is shown that the effect of amino-acid preparations (levamine-70-70, cerebrolysin, and aviamine) is dose-dependent. Thus, levamine-70 and cerebrolysin at 65 mg/kg do not affect the immune response but stimulate phagocytosis. Aviamine at 65 mg/kg inhibits the immune response but stimulates phagocytosis and in a dose of 6.5x10 -2 mg/kg boosts both processes.Key Words: levamine-70; cerebrolysin; aviamine; immune response; phagocytosisThe amino-acid preparations levamine-70 and cerebrolysin stimulate the thymus-dependent immune response when administered subcutaneously in mice in a range of low doses (6.5x10-~-6.5x10 -8 mg/kg) during 5 days [1]. The effect of these preparations on phagocytosis, a nonspecific index of resistance, has not been studied.Under clinical conditions levamine-70 and cerebrolysin are usually used in significantly higher doses, 0.65 mg/kg and 12.5 mg/kg, respectively.The aim of the present investigation was a comparative assessment of the effects of high and low doses of amino-acid preparations used in medical (levamine-70 and cerebrolysin) and veterinary (aviamine) practice on the thymus-dependent response and phagocytic activity of neutrophils (PAN) in mice. MATERIALS AND METHODSExperiments were carried out on 285 male CBA mice weighing 14-16 g. Finland), a mixture of isoleucine, leucine, lysine, methionine, phenylalanine, threonine, tryptophan, valine, histidine, arginine, alanine, proline, and glycine; cerebrolysin (Ebeve, Austria), a hydrolysate of brain tissue containing 18 amino acids, and aviamine (Drug Plant, St. Petersburg), a hydrolysate of hen blood casein containing 18 amino acids, were used.Cerebrolysin and aviamine were dosed by the protein content, which constituted 1 g/ml and 0.032 g/ml, respectively, whereas levamine-70 was dosed by the total content of amino acids (0.053 g /ml) and converted to kg of mass.The preparations dissolved in apyrogenic saline (Polfa, Poland) were injected s.c. in a range of doses 65.0-6.5x10 -1~ mg/kg during 5 days. Then some of the treated mice were immunized with