AMBRI biosensor: stabilizing artificial membranes and receptor attachment

Burns, Christopher J.; Braach-Maksvytis, Vijoleta; King, Laurie A.; Osman, Péter; Pace, Ronald; Raguse, Burkhard; Wieczorek, Lech; Cornell, Bruce

doi:10.1117/12.372925

Cited by 2 publications

(1 citation statement)

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“…The first system that falls into this category was an ionic channel switch sensor, which was later commercialized under the name AMBRI biosensors. − Strictly speaking, AMBRI biosensors were not based on conformational changes of receptors but rather on a configuration change of a complex system. The working principle of this sensor includes a gold electrode to which a lipid membrane with ion channels (gramicidin) and an anchored antibody are tethered.…”

Section: Overcoming Nonspecific Binding For Biosensorsmentioning

confidence: 99%

Nonspecific Binding—Fundamental Concepts and Consequences for Biosensing Applications

et al. 2021

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Nature achieves differentiation of specific and nonspecific binding in molecular interactions through precise control of biomolecules in space and time. Artificial systems such as biosensors that rely on distinguishing specific molecular binding events in a sea of nonspecific interactions have struggled to overcome this issue. Despite the numerous technological advancements in biosensor technologies, nonspecific binding has remained a critical bottleneck due to the lack of a fundamental understanding of the phenomenon. To date, the identity, cause, and influence of nonspecific binding remain topics of debate within the scientific community. In this review, we discuss the evolution of the concept of nonspecific binding over the past five decades based upon the thermodynamic, intermolecular, and structural perspectives to provide classification frameworks for biomolecular interactions. Further, we introduce various theoretical models that predict the expected behavior of biosensors in physiologically relevant environments to calculate the theoretical detection limit and to optimize sensor performance. We conclude by discussing existing practical approaches to tackle the nonspecific binding challenge in vitro for biosensing platforms and how we can both address and harness nonspecific interactions for in vivo systems.

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Section: Overcoming Nonspecific Binding For Biosensorsmentioning

confidence: 99%