Ameliorating effects of casein glycomacropeptide on obesity induced by high-fat diet in male Sprague-Dawley rats

Xu, Shi-Ping; Mao, Xueying; Cheng, Xue; Chen, Bin

doi:10.1016/j.fct.2013.01.027

Cited by 32 publications

(24 citation statements)

References 30 publications

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“…These studies suggest that GHP may improve the insulin sensitivity of insulin target organs. In our previous study, GHP could reduce the levels of interleukin-6 (IL-6), interleukin-1 beta (IL-1β) and tumor necrosis factor-alpha (TNF-α) in macrophages via Akt mediated nuclear factor-κB (NF-κB) signaling [19], and reduced pro-inflammatory cytokines in HFD-induced obesity rats [20], suggesting that GHP may have benefits on gut microbiota. Based on these results, we hypothesized that GHP could alleviate T2D by recovering insulin sensitivity and modulating gut microbiota.…”

Section: Of 15mentioning

confidence: 99%

Antidiabetic Effect of Casein Glycomacropeptide Hydrolysates on High-Fat Diet and STZ-Induced Diabetic Mice via Regulating Insulin Signaling in Skeletal Muscle and Modulating Gut Microbiota

et al. 2020

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This study evaluated the effects and the underlying mechanisms of casein glycomacropeptide hydrolysate (GHP) on high-fat diet-fed and streptozotocin-induced type 2 diabetes (T2D) in C57BL/6J mice. Results showed that 8-week GHP supplementation significantly decreased fasting blood glucose levels, restored insulin production, improved glucose tolerance and insulin tolerance, and alleviated dyslipidemia in T2D mice. In addition, GHP supplementation reduced the concentration of lipopolysaccharides (LPSs) and pro-inflammatory cytokines in serum, which led to reduced systematic inflammation. Furthermore, GHP supplementation increased muscle glycogen content in diabetic mice, which was probably due to the regulation of glycogen synthase kinase 3 beta and glycogen synthase. GHP regulated the insulin receptor substrate-1/phosphatidylinositol 3-kinase/protein kinase B pathway in skeletal muscle, which promoted glucose transporter 4 (GLUT4) translocation. Moreover, GHP modulated the overall structure and diversity of gut microbiota in T2D mice. GHP increased the Bacteroidetes/Firmicutes ratio and the abundance of S24-7, Ruminiclostridium, Blautia and Allobaculum, which might contribute to its antidiabetic effect. Taken together, our findings demonstrate that the antidiabetic effect of GHP may be associated with the recovery of skeletal muscle insulin sensitivity and the regulation of gut microbiota.

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Section: Of 15mentioning

confidence: 99%

Antidiabetic Effect of Casein Glycomacropeptide Hydrolysates on High-Fat Diet and STZ-Induced Diabetic Mice via Regulating Insulin Signaling in Skeletal Muscle and Modulating Gut Microbiota

et al. 2020

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“…The anti-inflammatory and immunomodulatory effects of GMP have been reported in rodent models of inflammatory bowel disease [23,24], murine spleen and bone marrow dendritic cells [25,26], as well as in lymphocytes and macrophages [27]. Until very recently, few animal studies have enlightened the capacity of GMP to attenuate metabolic disorders such as diabetes and dyslipidemia without addressing the underlying mechanisms [28][29][30]. Lately, Yuan et al [30] demonstrated that supplementing diabetic mice with a GMP hydrolysate significantly reduced fasting blood glucose, restored insulin production, improved insulin resistance (IR), increased skeletal muscle glycogen content, and reduced systemic inflammation while modifying the gut microbiota.To our knowledge, there is very little research focusing on the impact of GMP on oxidative stress (OxS) and inflammation pathways in enterocytes.…”

mentioning

confidence: 99%

Glycomacropeptide Prevents Iron/Ascorbate-Induced Oxidative Stress, Inflammation and Insulin Sensitivity with an Impact on Lipoprotein Production in Intestinal Caco-2/15 Cells

et al. 2020

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Background. Metabolic Syndrome (MetS), a major worldwide concern for the public health system, refers to a cluster of key metabolic components, and represents a risk factor for diabetes and cardiovascular diseases. As oxidative stress (OxS) and inflammation are the major triggers of insulin sensitivity (IS), a cardinal MetS feature, the principal aim of the present work is to determine whether glycomacropeptide (GMP), a milk-derived bioactive peptide, exerts beneficial effects on their expression. Methods. Fully differentiated intestinal Caco-2/15 cells are used to evaluate the preventive action of 2 mg/mL GMP against OxS and inflammation induced by the mixture iron-ascorbate (Fe/Asc) (200 μM:2 mM). The potency of GMP of decreasing the production of lipoproteins, including chylomicrons (CM), very-low-density lipoproteins (VLDL) and low-density lipoproteins (LDL) is also assessed. Results. The administration of GMP significantly reduces malondialdehyde, a biomarker of lipid peroxidation, and raises superoxide dismutase 2 and glutathione peroxidase via the induction of the nuclear factor erythroid 2–related factor 2, a transcription factor, which orchestrates cellular antioxidant defenses. Similarly, GMP markedly lowers the inflammatory agents tumor necrosis factor-α and cyclooxygenase-2 via abrogation of the nuclear transcription factor-kB. Moreover, GMP-treated cells show a down-regulation of Fe/Asc-induced mitogen activated protein kinase pathway, suggesting greater IS. Finally, GMP decreases the production of CM, VLDL, and LDL. Conclusions. Our results highlight the effectiveness of GMP in attenuating OxS, inflammation and lipoprotein biogenesis, as well as improving IS, the key components of MetS. Further investigation is needed to elucidate the mechanisms mediating the preventive action of GMP.

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“…Glycomacropeptide (GMP), a κ‐casein‐derived macropeptide f(106–169) comprised of 64 amino acids, has been reported to possess many bioactivities, such as reducing weight gain, decreasing cardiovascular disease risk markers, and improving intestinal flora . In our previous study, GMP reduces body weight of obese Sprague‐Dawley (SD) rats induced by high‐fat diet, and inhibits proliferation and differentiation of preadipocytes isolated from SD rats in vitro . Furthermore, our study shows that papain‐generated peptides from GMP suppress lipopolysaccharide‐induced inflammatory response in murine RAW 264.7 macrophages, and protect macrophages from hydrogen peroxide‐induced oxidative stress .…”

Section: Introductionmentioning

confidence: 76%

Casein glycomacropeptide‐derived peptide IPPKKNQDKTE ameliorates high glucose‐induced insulin resistance in HepG2 cells via activation of AMPK signaling

Song

Wang

et al. 2016

Molecular Nutrition Food Res

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Ameliorating effects of casein glycomacropeptide on obesity induced by high-fat diet in male Sprague-Dawley rats

Cited by 32 publications

References 30 publications

Antidiabetic Effect of Casein Glycomacropeptide Hydrolysates on High-Fat Diet and STZ-Induced Diabetic Mice via Regulating Insulin Signaling in Skeletal Muscle and Modulating Gut Microbiota

Antidiabetic Effect of Casein Glycomacropeptide Hydrolysates on High-Fat Diet and STZ-Induced Diabetic Mice via Regulating Insulin Signaling in Skeletal Muscle and Modulating Gut Microbiota

Glycomacropeptide Prevents Iron/Ascorbate-Induced Oxidative Stress, Inflammation and Insulin Sensitivity with an Impact on Lipoprotein Production in Intestinal Caco-2/15 Cells

Casein glycomacropeptide‐derived peptide IPPKKNQDKTE ameliorates high glucose‐induced insulin resistance in HepG2 cells via activation of AMPK signaling

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