Ameliorating effects of curcumin-loaded superparamagnetic iron oxide nanoparticles (SPIONs) on the mouse testis exposed to the transient hyperthermia: A molecular and stereological study

Khosravi, Amir; Hasani, Amirhosein; Rahimi, Kimia; Aliaghaei, Abbas; Pirani, Maryam; Azad, Nahid; Ramezani, Fahim; Tamimi, Atena; Behnam, Paria; Raoofi, Amir; Fathabadi, Fatemeh Fadaei; Abdi, Shabnam; Abdollahifar, Mohammad‐Amin; Hejazi, Fatemeh

doi:10.1016/j.acthis.2020.151632

Cited by 12 publications

(7 citation statements)

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“…In vitro analyses on Leydig cell lines have indicated that curcumin exerts a protective effect against a variety of stressors mainly by suppressing apoptosis and also alleviating oxidative stress and endoplasmic reticulum stress ( Chen et al 2019 , 2020 , Yildizbayrak & Erkan 2019 ). When assessed in vivo , curcumin’s protective role translates into preserved testicular volume, tubular epithelium height, tubule length, and number of viable Leydig and germ cells ( Noorafshan et al 2011 , Lin et al 2015 , Mahmoudi et al 2017 , Chen et al 2019 , Khosravi et al 2020 , Afshar et al 2021 ). In the present report, we show that curcumin reduces basal and H 2 O 2 -stimulated ROS levels in MA-10 Leydig cells at low concentrations.…”

Section: Discussionmentioning

confidence: 99%

“…; Yildizbayrak & Erkan 2019). When assessed in vivo, curcumin's protective role translates into preserved testicular volume, tubular epithelium height, tubule length, and number of viable Leydig and germ cells(Noorafshan et al 2011;Lin et al 2015;Mahmoudi et al 2017;Chen et al 2019;Khosravi et al 2020;Afshar et al proposed as a modulator of the Nrf2 pathway in the testis, a transcription factor that regulates the expression of several antioxidant enzymes(Ashrafizadeh et al 2020). An in vivo study in rats has shown that…”

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Curcumin effects on Leydig cell functions and potential therapeutic uses

et al. 2023

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Curcumin has been ascribed with countless therapeutic effects, but its impact on testicular function has been scarcely researched. Leydig cells comprise the androgen-secreting population of the testis and may give rise to Leydig cell tumours (LCT). Due to their steroid-secreting nature, LCT entail endocrine, reproductive, and psychological disorders. Approximately 10% are malignant and do not respond to chemotherapy and radiotherapy. The aim of this study was to assess curcumin’s impact on Leydig cells functions and its potential effect on LCT growth. In vitro assays on MA-10 Leydig cells showed that curcumin (20 to 80 µmol/L) stimulates acute steroidogenesis, both in the presence and absence of db-cAMP. This effect is accompanied by an increase in StAR expression. Regarding curcumin’s in vitro cytostatic capacity, we show that 40 to 80 µmol/L curcumin reduces MA-10 Leydig cells’ proliferative capacity, which could be explained by the arrest in G2/M and the reduced viability due to the activation of the apoptotic pathway. Finally, CB6F1 mice were inoculated with MA-10 cells to generate ectopic LCT in both flanks. They received intraperitoneal injections of 20 mg/kg curcumin or vehicle every other day for 15 days. We unveiled curcumin’s capacity to inhibit LCT growth as evidenced by reduced tumour volume, weight, and area under the growth curves. No detrimental effects on general health parameters or testicular integrity were observed. These results provide novel evidence on curcumin’s effects on the endocrine cell population of the testis and propose this natural compound as a therapeutic agent for LCT.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Curcumin effects on Leydig cell functions and potential therapeutic uses

et al. 2023

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“…The authors found that the prepared nanoparticles were able to deliver after 6 h, as shown by the increase of the apoptotic cells and of the caspase 3 expression. The preparation of SPIONs loaded with quercetin by means of a nanoprecipitation method has also been reported in the literature [ 392 ]. Wistar male rats were orally gavage fed with quercetin, either loaded in SPIONs or in its free form at 50 and 100 mg/kg daily doses for 7 days.…”

Section: Inorganic-based Delivery Systemsmentioning

confidence: 99%

Innovative Delivery Systems Loaded with Plant Bioactive Ingredients: Formulation Approaches and Applications

Kyriakoudi

Spanidi²,

Mourtzinos

et al. 2021

Plants

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Plants constitute a rich source of diverse classes of valuable phytochemicals (e.g., phenolic acids, flavonoids, carotenoids, alkaloids) with proven biological activity (e.g., antioxidant, anti-inflammatory, antimicrobial, etc.). However, factors such as low stability, poor solubility and bioavailability limit their food, cosmetics and pharmaceutical applications. In this regard, a wide range of delivery systems have been developed to increase the stability of plant-derived bioactive compounds upon processing, storage or under gastrointestinal digestion conditions, to enhance their solubility, to mask undesirable flavors as well as to efficiently deliver them to the target tissues where they can exert their biological activity and promote human health. In the present review, the latest advances regarding the design of innovative delivery systems for pure plant bioactive compounds, extracts or essential oils, in order to overcome the above-mentioned challenges, are presented. Moreover, a broad spectrum of applications along with future trends are critically discussed.

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“…Apoptosis of testicular germ cells plays a crucial role in both testicular development and the destruction of germ cells under physiological and pathological conditions [8,9]. Prior research has demonstrated that hyperthermia triggers cell death in the testis, leading to a noticeable death of primary spermatocytes and spermatogonia [10][11][12]. Various drugs, with distinct mechanisms, have been employed to mitigate the impact of heat stress on spermatogenic cells in testicular tissue.…”

Section: Introductionmentioning

confidence: 99%

Caffeine attenuates spermatogenic disorders in mice with induced chronic scrotal hyperthermia

Raoofi,

Gholami,

Mokhtari

et al. 2024

Clin Exp Reprod Med

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Objective: Chronic scrotal hyperthermia (SHT) can lead to serious disorders of the male reproductive system, with oxidative stress playing a key role in the onset of these dysfunctions. Thus, we evaluated the impact of caffeine, a potent antioxidant, on cellular and tissue disorders in mice with chronic SHT.Methods: In this experimental study, 56 adult male NMRI mice were allocated into seven equal groups. Apart from the non-treated control group, all were exposed to heat stress. Two groups, termed “preventive” and “curative,” were orally administered caffeine. The preventive mice began receiving caffeine immediately prior to heat exposure, while for the curative group, a caffeine regimen was initiated 15 consecutive days following cessation of heat exposure. Each treated group was subdivided based on pairing with a positive control (Pre/curative [Cur]+PC) or a vehicle (Pre/Cur+vehicle). Upon conclusion of the study, we assessed sperm characteristics, testosterone levels, stereological parameters, apoptosis, antioxidant and oxidant levels, and molecular markers.Results: Sperm parameters, testosterone levels, stereological parameters, biochemical factors (excluding malondialdehyde [MDA]), and c-kit gene expression were significantly elevated in the preventive and curative groups, especially the former, relative to the other groups. Conversely, expression levels of the heat shock protein 72 (HSP72) and nuclear factor kappa beta (NF-κβ) genes, MDA levels, and apoptotic cell density were markedly lower in both caffeine-treated groups relative to the other groups, with more pronounced differences observed in the preventive group. Conclusion: Overall, caffeine attenuated cellular and molecular abnormalities induced by heat stress in the testis, particularly in the mice treated under the preventive condition.

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Ameliorating effects of curcumin-loaded superparamagnetic iron oxide nanoparticles (SPIONs) on the mouse testis exposed to the transient hyperthermia: A molecular and stereological study

Cited by 12 publications

References 59 publications

Curcumin effects on Leydig cell functions and potential therapeutic uses

Curcumin effects on Leydig cell functions and potential therapeutic uses

Innovative Delivery Systems Loaded with Plant Bioactive Ingredients: Formulation Approaches and Applications

Caffeine attenuates spermatogenic disorders in mice with induced chronic scrotal hyperthermia

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