Ameliorating effects of green tea extract on cadmium induced reproductive injury in male Wistar rats with respect to androgen receptors and caspase- 3

Abdelrazek, Heba M. A.; Helmy, Seham A.; Elsayed, Doaa H.; Ebaid, Hala M.; Mohamed, Racha M.

doi:10.1016/j.repbio.2016.11.001

Cited by 63 publications

(45 citation statements)

References 42 publications

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“…In the field of toxicology, reproductive dysfunction and cancer are the adverse effects of greatest concern (Abdelrazek et al, 2016). It has been assumed that fifty percent of human male infertility of unknown cause may be due to environmental and occupational exposures (Evenson and Wixon, 2005).…”

Section: Discussionmentioning

confidence: 99%

Garlic Powder Attenuates Apoptosis Associated with Lead Acetate-Induced Testicular Damage in Adult Male Rats

Nasr¹,

Elmadawy²,

Almadaly³

et al. 2017

AJVS

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Key words:Lead toxicity, apoptosis, male rat, testis, garlic Lead is a highly prevalent heavy metal pollutant in human and animals which has various toxicological effects on the different organs including reproductive system on particular aspect the male testis. Lead (Pb) exposure enhances the generation of Reactive Oxygen Species (ROS) which negatively affect testis structure and functions. The present study aimed to investigate the effect of garlic supplement on daily basis and its prophylactic possibility against lead acetate induced testicular dysfunction. Thirty two adult male rats were divided to four groups, eight animals per each. All animals were maintained under standard laboratory conditions with free access to suitable diet and water supply. The 1 st group was supplied with water and diet and kept without further treatment as a negative control. The 2 nd group was treated by lead acetate( 50mg/L) daily in drinking water as a positive control. The 3 rd group was administered commercial garlic powder preparation only (200 mg/kg b.w.) by stomach tube. The 4 th group was co-treated with lead acetate in drinking water plus garlic in same doses by stomach tube. Then, all animals were scarified after 42 days for clean excision of epididymis and testes. Semen was collected and analyzed for count, motility and viability. Testicular tissues sections were prepared, stained and examined. Oxidative stress markers, SOD and MDA were determined in testicular tissue homogenate. Immunostaining was carried out on sections of testicular tissue for detection of proapoptotic markers caspase-3 and Bax expression. Finally, the level of expression of Bcl-2 and p53 genes was measured in testicular tissue samples as well. The data summary obtained from co treated group signified the protective effect of garlic against potential lead acetate induced undesired testicular apoptosis.

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Section: Discussionmentioning

confidence: 99%

Garlic Powder Attenuates Apoptosis Associated with Lead Acetate-Induced Testicular Damage in Adult Male Rats

Nasr¹,

Elmadawy²,

Almadaly³

et al. 2017

AJVS

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“…Several studies report that Cd exposure produces harmful effects on prostate, testicle and sperm [6, 8, 13]. The orally LD 50 of CdCl 2 is suggested to be 90 mg/kg [14]. However, the effects of relative low dose Cd exposure on the proliferation and apoptosis of prostate cells are not consistent.…”

Section: Discussionmentioning

confidence: 99%

“…Daily exposed to Cd for a long time stimulates prostatic inflammation and stromal fibrillar rearrangement, but does not promote the proliferation of prostatic cells [7]. Abdelrazek et al found that Cd reduced the prostate weight via promoting cells apoptosis [14]. A possible hypothesis is that short-time Cd exposure induces the apoptosis of prostate cells, while chronic exposure to Cd may stimulate cells growth.…”

Section: Discussionmentioning

confidence: 99%

Potential Ameliorative Effects of Qing Ye Dan Against Cadmium Induced Prostatic Deficits via Regulating Nrf-2/HO-1 and TGF-β1/Smad Pathways

Lei

Chen

et al. 2017

Cell Physiol Biochem

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Background/Aims: Cadmium (Cd) is an environmental pollutant with reproductive toxicity. Swertia mileensis is used in Chinese medicine for the treatment of prostatic deficits and named as Qing Ye Dan (QYD). This study was undertaken to investigate the potential protective effects of QYD against Cd-induced prostatic deficits. Method: Rat model of prostatic deficits was induced by 0.2 mg/kg/d CdCl₂ subcutaneous injection for 15 days. The prostatic oxidative stress was evaluated by detecting the levels of malondialdehyde, nitric oxide, reduced/ oxidized glutathione, total sulfhydryl groups and enzymatic antioxidant status. The prostatic inflammation was estimated by testing the levels of pro-inflammatory cytokines. The levels of epithelial-mesenchymal transition (EMT) markers E-cadherin, fibronectin, vimentin and α-smooth muscle actin were measured by qPCR analysis. Additionally, the prostatic expressions of transforming growth factor-β1 (TGF-β1), type I TGF-β receptor (TGF-βRI), Smad2, phosphorylation-Smad2 (p-Smad2), Smad3, p-Smad3, Smad7, nuclear related factor-2 (Nrf-2), heme oxygenase-1 (HO-1), B-cell CLL/lymphoma (Bcl)-2 and Bcl-2-associated X protein (Bax) were measured by western blot assay. Results: It was found that QYD ameliorated the Cd-induced prostatic oxidative stress and inflammation, attenuated prostatic EMT, inhibited the TGF-β1/Smad pathway, increased Bcl-2/Bax ratio and enhanced the activity of Nrf-2/HO-1 pathway. Conclusion: These results showed that QYD could ameliorate Cd-induced prostatic deficits via modulating Nrf-2/HO-1 and TGF-β1/Smad pathways.

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“…Green tea polyphenols can scavenge FR and protect cells from oxidative damage (Al‐Gnami, ; Chacko et al, ; Hamden et al, ). Among many favorable effects of green and black tea extract consumption there is also prevention from the harmful impact of Cd on the liver, serum and testes (Abdelrazek, Helmy, Elsayed, Ebaid, & Mohamed, ; Al‐Gnami, ; Hamden et al, ; Mantur et al, ; Winiarska‐Mieczan, )…”

Section: Polyphenol‐rich Products In Protection and Treatment Of Cd‐imentioning

confidence: 99%

“…Green tea polyphenols can scavenge FR and protect cells from oxidative damage (Al-Gnami, 2014;Chacko et al, 2010;Hamden et al, 2008). Among many favorable effects of green and black tea extract consumption there is also prevention from the harmful impact of Cd on the liver, serum and testes(Abdelrazek, Helmy, Elsayed, Ebaid, & Mohamed, 2016;Al-Gnami, 2014;Hamden et al, 2008;Mantur et al, 2014;Winiarska-Mieczan, 2015) It has been shown that p.o. infusion of 5% aqueous green tea extract to Wistar rats exposed to Cd at levels of 2.25 mg/kg bw, subcutaneously (s.c.) for 6 months decreased the activities of LDH, GGT, acid phosphatase and ALP, and the concentration of bilirubin in the serum, as well as the concentration of TBARS in the liver, and enhanced the activities of CAT, SOD and GPx in this organ compared to the animals receiving Cd alone(Hamden et al, 2008).…”

mentioning

confidence: 99%

Review of polyphenol‐rich products as potential protective and therapeutic factors against cadmium hepatotoxicity

Mężyńska

Brzóska

2018

J of Applied Toxicology

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Recently, the growing attention of the scientific community has been focused on the threat to health created by environmental pollutants, including toxic metals such as cadmium (Cd), and on the need of finding effective ways to prevent and treat the unfavorable health effects of exposure to them. Particularly promising for Cd, and thus arousing the greatest interest, is the possibility of using various ingredients present in plants, including mainly polyphenolic compounds. As the liver is one of the target organs for this toxic metal and disturbances in the proper functioning of this organ have serious consequences for health, the aim of the present review was to discuss the possibility of using polyphenol-rich food products (e.g., chokeberry, black and green tea, blueberry, olive oil, rosemary and ginger) as the strategy in protection from this xenobiotic hepatotoxicity and treatment of this heavy metal-induced liver damage. Owing to the ability of polyphenols to bind ions of Cd and the strong antioxidative potential of these compounds, as well as their abundance in dietary products, it seems to be of high importance to consider the possibility of using polyphenols as potential preventive and therapeutic agents against Cd hepatotoxicity, determined by its strong pro-oxidative properties. Although most of the data on the effectiveness of polyphenols comes from studies in animals, the fact that some of them are derived from experimental models that reflect human exposure to this metal allows us to assume that some polyphenol-rich food products may be promising protective agents against Cd hepatotoxicity in humans.

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Ameliorating effects of green tea extract on cadmium induced reproductive injury in male Wistar rats with respect to androgen receptors and caspase- 3

Cited by 63 publications

References 42 publications

Garlic Powder Attenuates Apoptosis Associated with Lead Acetate-Induced Testicular Damage in Adult Male Rats

Garlic Powder Attenuates Apoptosis Associated with Lead Acetate-Induced Testicular Damage in Adult Male Rats

Potential Ameliorative Effects of Qing Ye Dan Against Cadmium Induced Prostatic Deficits via Regulating Nrf-2/HO-1 and TGF-β1/Smad Pathways

Review of polyphenol‐rich products as potential protective and therapeutic factors against cadmium hepatotoxicity

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