Amelioration of 5-fluorouracil-induced intestinal mucositis by Streptococcus thermophilus ST4 in a mouse model

Shen, Siou-Ru; Chen, Wei-Jen; Chu, Hui-Fang; Wu, Shiuan-Huei; Wang, Yu-Ru; Shen, Tang‐Long

doi:10.1371/journal.pone.0253540

Cited by 12 publications

(20 citation statements)

References 53 publications

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“…In agreement, it has been shown that a yogurt diet rich in acetate could improve the protective function of the intestinal epithelium [ 92 ]. Likewise STH strain ST4 increases the fecal content of acetate in correlation with maintenance of inflammatory homeostasis and preservation of intestinal permeability in a mucositis induced model [ 61 ].…”

Section: Resultsmentioning

confidence: 99%

“…Proteolysis of caseins by yogurt bacteria including STH liberates bioactive peptides that, among others, stimulate mucin gene expression and secretion in vitro and enhance the number of goblet and Paneth cells along the small intestine and the expression of intestinal mucins and antibacterial factors in vivo [ 60 ]. Also, it has been proposed that the positive effect of STH strain ST4 on mice with 5-fluorouracil-induced mucositis is attributed to an increase in the content of fecal short chain fatty acid (SCFA) acetate, in correlation with maintenance of inflammatory homeostasis and preservation of intestinal permeability [ 61 ]. Finally, folate produced by STH would be involved in the reduction of inflammation in rodent models of induced mucositis [ 46 , 62 – 64 ].…”

Section: Introductionmentioning

confidence: 99%

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The genomic basis of the Streptococcus thermophilus health-promoting properties

et al. 2022

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Background Streptococcus thermophilus is a Gram-positive bacterium widely used as starter in the dairy industry as well as in many traditional fermented products. In addition to its technological importance, it has also gained interest in recent years as beneficial bacterium due to human health-promoting functionalities. The objective of this study was to inventory the main health-promoting properties of S. thermophilus and to study their intra-species diversity at the genomic and genetic level within a collection of representative strains. Results In this study various health-related functions were analyzed at the genome level from 79 genome sequences of strains isolated over a long time period from diverse products and different geographic locations. While some functions are widely conserved among isolates (e.g., degradation of lactose, folate production) suggesting their central physiological and ecological role for the species, others including the tagatose-6-phosphate pathway involved in the catabolism of galactose, and the production of bioactive peptides and gamma-aminobutyric acid are strain-specific. Most of these strain-specific health-promoting properties seems to have been acquired via horizontal gene transfer events. The genetic basis for the phenotypic diversity between strains for some health related traits have also been investigated. For instance, substitutions in the galK promoter region correlate with the ability of some strains to catabolize galactose via the Leloir pathway. Finally, the low occurrence in S. thermophilus genomes of genes coding for biogenic amine production and antibiotic resistance is also a contributing factor to its safety status. Conclusions The natural intra-species diversity of S. thermophilus, therefore, represents an interesting source for innovation in the field of fermented products enriched for healthy components that can be exploited to improve human health. A better knowledge of the health-promoting properties and their genomic and genetic diversity within the species may facilitate the selection and application of strains for specific biotechnological and human health-promoting purpose. Moreover, by pointing out that a substantial part of its functional potential still defies us, our work opens the way to uncover additional health-related functions through the intra-species diversity exploration of S. thermophilus by comparative genomics approaches.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The genomic basis of the Streptococcus thermophilus health-promoting properties

et al. 2022

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“…[24,25] In addition, it has been seen that 5-FU causes damage in different cells and activates apoptosis in them by increasing active oxygen species, which has been investigated and confirmed in various studies in mice. [26][27][28] Due to the innovative results of TQ in various health conditions, including free radical scavenging, antioxidant, and anti-inflammatory activities, this study investigated the possible protective effect of 2.5 mg/kg of TQ against 5-FU-induced oral and IM and its underlying mechanisms of action in mice. Also, according to the antitumor properties that thymoquinone has been seen against various types of cancer, in addition to its role in reducing mucositis, it can create a synergistic effect with 5-FU.…”

Section: Discussionmentioning

confidence: 99%

Thymoquinone protects against 5‐Fluorouracil‐induced mucositis by NF‐κβ and HIF‐1 mechanisms in mice

Madani

Kazemi

Shirafkan

et al. 2023

J Biochem & Molecular Tox

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Mucositis is among the most common side effects of 5‐Fluorouracil (5‐FU) and other cancer therapeutic drugs. Thymoquinone (TQ), a bioactive constituent extracted from Nigella sativa, has antioxidant and anti‐inflammatory properties and can modify acute gastrointestinal injury. To investigate the effects of TQ on mucositis induced by 5‐FU, studied animals were divided into four groups: control, 5‐FU unit dose (300 mg/kg) to cause oral and intestinal mucositis (OM and IM), TQ (2.5 mg/kg) and TQ (2.5 mg/kg) plus 5‐FU. Due to The molecular mechanisms, it was confirmed that the expression of NF‐κβ and HIF‐1 increases in OM. The serum levels of malondialdehyde (MDA), catalase (CAT), and superoxide dismutase (SOD), as well as pathological parameters, were assessed. Based on our results, the nuclear factor‐kappa β gene expression in the tongue was downregulated significantly in the 5‐FU + TQ compared to the 5‐FU. TQ treatment can diminish MDA, and a reduction in oxidative stress was shown. TQ could also reduce the severity of tissue destruction and damaging effects induced by 5‐FU on the tongue and intestine. We also observed lower villus length and width in the intestine of the 5‐FU group compared to the control group. According to our research's pathological, biochemical, and molecular results, treatment with TQ as an anti‐inflammatory and antioxidant compound may be the potential to improve and treat 5‐FU‐induced OM and IM, and TQ could be used against cancer treatment drugs and exhibit fewer adverse effects.

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“…The GIT mucosa in addition to having an absorptive role, it acts as a physical and immunological barrier. [19,20] Many studies have confirmed that the injury of intestinal mucosal caused by 5-FU-harms the intestinal barrier resulting in invasion of the gut flora into the circulation that lead to systemic inflammatory response [21] .…”

Section: Discussionmentioning

confidence: 99%

Intestinal Mucositis Induced by 5- Fluorouracil and the Possible Protective Effect of Probiotics Containing Lactobacillus in Adult Male Albino Rats: Histological and Immunohistochemical Study

Shaheen

Mousa²

2022

Benha Medical Journal

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Introduction:Intestinal mucositis is the most prevalent side effect of antineoplastic drug 5-flurouoracil (5-FU). Probiotics are live microorganisms that improve intestinal immune regulation. So, probiotics might have a beneficial effect to reduce the chemotherapy-induced enterotoxicity. Aim: This study aimed to assess mucositis induced by 5-FU and the possible protective effect of probiotics. Methods: 50 healthy albino adult male rats were divided into five equivalent groups. Control group, probiotic group was given 100 µL of suspension containing 1x10 7 (CFU) of probiotic cocktail daily for 9 days, 5-FU group was received 50 mg/kg of 5-FU by intraperitoneal (IP) injection once daily for 5 consecutive days, probiotic plus 5-FU group was given 50 mg/kg of 5-FU by IP injection for 5 consecutive days with probiotic cocktail once daily for 9 consecutive days and recovery group was received 50 mg/kg 5-FU by IP injection once daily for 5 consecutive days then left without medication till rats had been sacrificed on 9th day .The intestinal tissues have been prepared for a light microscopic study. Results: Rats of 5-FU group showed broadening, fusion and sloughing of villi into lumen, ulcerations, inflammatory infiltration, marked depletion of goblet cells, strong iNOS, marked caspase 3 and weak Ki-67 immuno-expressions. Rats treated with 5-FU plus probiotic showed preservation of normal intestinal architecture with improvement of histological and immune-histological features of mucositis. Rats of recovery group showed decrease histological and immune-histochemical features of mucositis. Conclusion: oral intake of the probiotic could protect against 5-FU induced intestinal mucositis in a rat model.

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Amelioration of 5-fluorouracil-induced intestinal mucositis by Streptococcus thermophilus ST4 in a mouse model

Cited by 12 publications

References 53 publications

The genomic basis of the Streptococcus thermophilus health-promoting properties

The genomic basis of the Streptococcus thermophilus health-promoting properties

Thymoquinone protects against 5‐Fluorouracil‐induced mucositis by NF‐κβ and HIF‐1 mechanisms in mice

Intestinal Mucositis Induced by 5- Fluorouracil and the Possible Protective Effect of Probiotics Containing Lactobacillus in Adult Male Albino Rats: Histological and Immunohistochemical Study

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