Amelioration of Alcohol Induced Gastric Ulcers Through the Administration of Lactobacillus plantarum APSulloc 331261 Isolated From Green Tea

Abstract

Gastric inflammation is an indication of gastric ulcers and possible other underlying gastric malignancies. Epidemiological studies have revealed that several Asian countries, including South Korea, suffer from a high incidence of gastric diseases derived from high levels of stress, alcoholic consumption, pyloric infection and usage of non-steroidal anti-inflammatory drugs (NSAIDs). Clinical treatments of gastric ulcers are generally limited to proton pump inhibitors that neutralize the stomach acid, and the application of antibiotics for Helicobacter pylori eradication, both of which are known to have a negative effect on the gut microbiota. The potential of probiotics for alleviating gastrointestinal diseases such as intestinal bowel syndrome and intestinal bowel disease receives increasing scientific interest. Probiotics may support the amelioration of disease-related symptoms through modulation of the gut microbiota without causing dysbiosis. In this study the potential of Lactobacillus plantarum APSulloc 331261 (GTB1 TM ), isolated from green tea, was investigated for alleviating gastric inflammation in an alcohol induced gastric ulcer murine model (positive control). Treatment with the test strain significantly influenced the expression of pro-inflammatory and anti-inflammatory biomarkers, interleukin 6 (IL6) and interleukin 10 (IL10), of which the former was downand the latter up-regulated when the alcohol induced mice were treated with the test strain. This positive effect was also indicated by less severe gastric morphological changes and the histological score of the gastric tissues. A significant increase in the abundance of Akkermansia within the GTB1 TM treated group compared to the positive control group also correlated with a decrease in the ratio of acetate over propionate. The increased levels of propionate in the GTB1 TM group appear to result from the impact of the test strain on the microbial population and the resulting metabolic activities. Moreover, there was a significant increase in beta-diversity in the group that received GTB1 TM over that of the alcohol induced control group.

Impact of Dietary Patterns on H. pylori Infection and the Modulation of Microbiota to Counteract Its Effect. A Narrative Review

“…In a mouse model that combined ethanol and H. pylori administration, Park et al treated the animals with green tea-derived L. plantarum , concluding that these probiotics decreased inflammation and improved gastric ulcers, possibly by restoring healthy gut microbiota [ 119 ]. In another study by the same group, mice were fed L. plantarum before H. pylori infection.…”

Lactate as a metabolite from probiotic Lactobacilli mitigates ethanol-induced gastric mucosal injury: an in vivo study

“…Caspase 3 is the key executor protein for the caspases cascade reaction of apoptosis, interacting with caspase-8 and caspase-9 [9,10]. Numerous in vivo studies have reported that many genera of probiotic Lactobacilli had protective effects on the gastric mucosa; the underlying mechanisms, however, remain to be fully elucidated [11][12][13][14][15][16]. Lactate is a major metabolite of Lactobacilli that has been widely used as a food additive for human [17,18].…”

Antibacterial and Antifungal Activity of Lactobacillus Plantarum Isolated from Green Tea

“…Moreover, it markedly increased the abundance of various intestinal bene cial bacteria such as Bi dobacterium spp. and Clostridium butyricum [33]. Furthermore, L. plantarum APsulloc 331261-derived extracellular vesicles exerted anti-in ammatory effects on human skin by inducing macrophage polarisation [34].…”

“…L. plantarum strains APsulloc 331261 and 331266 isolated from green tea (Camellia sinensis) have been sequenced and annotated using whole-genome sequence analysis, and their safety and bene ts have also been described [32]. L. plantarum APsulloc 331261 alleviated gastric in ammation in an alcoholinduced gastric ulcer murine model [33]. Moreover, it markedly increased the abundance of various intestinal bene cial bacteria such as Bi dobacterium spp.…”