Amelioration of hyperglycaemia and modulation of antioxidant status by Alcea rosea seeds in alloxan-induced diabetic rats

Abstract

Context:Alcea rosea L. (Malvaceae) has various medicinal uses including anticancer, anti-inflammatory and analgesic properties. However, there is no report on its antidiabetic activity.Objective:Alcea rosea seed extracts were evaluated for antihyperglycaemic and antioxidative potential in diabetic rats.Materials and methods: Single intra-peritoneal injection of alloxan (130 mg/kg b.w.) was used for induction of diabetes in Albino Wistar rats. Antihyperglycaemic and antioxidant activities of methanol and aqueous extracts of Alcea rosea seed (100 and 300 mg/kg b.w.), administered orally on daily basis for 15 days, were assessed in vivo for fasting blood glucose level and antioxidant status of liver and pancreas. Metformin was used as a positive control.Results: Aqueous and methanol extracts (300 mg/kg b.w.) decreased blood glucose level in diabetic rats by 24% and 46%, respectively. Administration of aqueous and methanol extracts at 300 mg/kg b.w. significantly (p < 0.01) modulated the antioxidant status of liver in diabetic rats by increasing levels of GR (22.5 ± 1.0, 24.4 ± 1.02 μg GSSG utilized/min/mg of protein), GPx (20.7 ± 1.2, 23.6 ± 2.04 μg GSH utilized/min/mg of protein), SOD (36.1 ± 1.7, 39.05 ± 1.5 units/mg of protein) and CAT (1744.5 ± 132.5, 1956.6 ± 125.2 nmol H2O2 decomposed/min/mg of protein), respectively. Similar results were observed for pancreas.Discussion and conclusions: Antihyperglycaemic and antioxidative potentials of Alcea rosea seeds suggest its usefulness in management of diabetes and its complications. This is the first report on antidiabetic activity of this plant.

Antioxidant effects of Moringa oleifera seed oil against oxidative stress induced by alloxan in rats.

“…Increased levels of CAT, GSH and SOD against ROS produced by Alloxan induction is owing to the presence of large amounts of antioxidants such as tocopherols, phenolic compounds, and flavonoids, specifically quercetin, kaempferol and chlorogenic acid in MO seed oil that have scavenging potential against ROS. It was also found that Alloxan induction in group II rats markedly increased hepatic MDA levels, which is indicative of increased oxidative stress: these findings correlate with the previous studies 36 . Group treated with the Alloxan+MO seed oil showed significantly lower MDA levels than the Alloxan treated diabetic group (Table 5).…”

Gastroprotective and Immuno-supportive Role of Alcea kurdica against Stress Induced Lesion in Japanese Quails

“…Following the 15 th and 30 th days of treating groups with additives, blood sugar was nonsignificantly lower in A.kurdica (T3) group than that of (T1), (T1), (T0+), and (T0-), respectively. The anti-diabetic activity of Alcea genus was previously shown in alloxan-induced diabetic rates 26 . The hypoglycemic effect of A.kurdica can be linked with their antioxidant activity and phenolic contents that have been reported by Dar et al 27 (Table 1).…”

Extraction of polysaccharide from Althea rosea and its physicochemical, anti-diabetic, anti-hypertensive and antioxidant properties

“…Browning is proposed to be due to oxidative stress following high-temperature exposure. A.rosea possess high total phenolic content 26 , and oxidation of phenolic compounds possibly will result in a deep brown color of the mucilage. Furthermore, extraction temperature-induced browning in the color of the polysaccharide/mucilage.…”