Amelioration of streptozotocin-induced type 2 diabetes mellitus in Wistar rats by arachidonic acid

Gundala, Naveen K.V.; Naidu, V.G.M.; Das, Undurti N.

doi:10.1016/j.bbrc.2018.01.007

Cited by 64 publications

(47 citation statements)

References 43 publications

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“…Finally, AA also facilitates the production of anti-inflammatory lipoxins. The latter were reported to improve insulin sensitivity and may prevent the development of DM [ 157 ]. For instance, LXA4 inhibits IL-6, TNF-α, and ROS production thus hampers obesity-associated inflammation and has an anti-diabetic effect [ 126 , 158 , 159 , 160 ].…”

Section: Aa Metabolism In the Pathogenesis Of Diabetes Mellitusmentioning

confidence: 99%

Arachidonic Acid Metabolites in Cardiovascular and Metabolic Diseases

Sonnweber

Pizzini

Nairz

et al. 2018

IJMS

320

221

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Lipid and immune pathways are crucial in the pathophysiology of metabolic and cardiovascular disease. Arachidonic acid (AA) and its derivatives link nutrient metabolism to immunity and inflammation, thus holding a key role in the emergence and progression of frequent diseases such as obesity, diabetes, non-alcoholic fatty liver disease, and cardiovascular disease. We herein present a synopsis of AA metabolism in human health, tissue homeostasis, and immunity, and explore the role of the AA metabolome in diverse pathophysiological conditions and diseases.

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Section: Aa Metabolism In the Pathogenesis Of Diabetes Mellitusmentioning

confidence: 99%

Arachidonic Acid Metabolites in Cardiovascular and Metabolic Diseases

Sonnweber

Pizzini

Nairz

et al. 2018

IJMS

320

221

View full text Add to dashboard Cite

show abstract

“…Streptozotocin was purchased in the institute by Sigma-Aldrich, St. Louis, USA whereas Glibenclamide was bought from Sanofi India Ltd. All the chemicals used were of analytical grade. This was used for inducing type 2 diabetes (Gundala et al, 2018).…”

Section: Chemicalsmentioning

confidence: 99%

Anti-diabetic, diabetic neuropathy protective action and mechanism of action involving oxidative pathway of chlorogenic acid isolated from Selinum vaginatum roots in rats

Saraswat

Sachan

Chandra

2020

Heliyon

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Phytopharmaceuticals have always reported vital roles in the field of medicine hence the need to investigate safe and efficient drugs for treating metabolic disorders is very significant. Roots of Selinum vaginatum have therapeutic benefits and are widely used by the people of the Rohtang region for treating diabetes and its associated complications. The present study focusses on the isolation of the bioactive from the S. vaginatum roots for estimating acute toxicity studies, anti-diabetic and diabetic neuropathy protective action along with the mechanism of action in STZ induced Wistar rats. The Selinum vaginatum roots were collected from the Rohtang region, Himalayas. Chlorogenic acid was isolated and underwent identification by UV, HPLC, 1 H NMR, C13 NMR, Mass, and FTIR spectroscopy methods. Chlorogenic acid was dosed at 10 and 20 mg/kg to observe the effects on experimentally induced diabetes and with time generated diabetic neuropathic complications. Biomarkers TNF-α, superoxide dismutase, nitrosative stress, lipid peroxide profile, and membrane-bound inorganic phosphate were analyzed. Histopathological evaluation of the liver and sciatic nerve was performed for all groups. Parameters like blood glucose levels, body weight, food intake, Thermal Hyperalgesia, Writhing, Cold Hyperalgesia Responses, Mechanical hyperalgesia, Grip Strength, Spontaneous Locomotor (Exploratory) Test, Neuromuscular Coordination tests, and lipid profile analysis showcased the anti-diabetic and diabetic neuropathy protective action of the drug. Inflammation, degradation, and necrosis were found to be reduced in the liver and sciatic nerve cells of treated groups. All the biomarkers used to analyze the oxidative pathway were significantly replenished indicates that chlorogenic acid produces these effects through this pathway.

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“…Male Wistar rats, fed with a high-fat diet and treated with a low dose of STZ (30-40 mg/ kg body weight) is reported to be a reliable model for human type 2 diabetes symptoms, which include peripheral insulin resistance, increased plasma interleukin-6 (IL-6) and hyperglycemia [13,14]. This model, reported to mimic the development of type 2 diabetes and its metabolic features, has been used by various researchers to not only investigate mechanisms involved but also evaluate potential therapies [13,14].…”

Section: Introductionmentioning

confidence: 99%

Anti-hyperglycemic activity of myricetin, through inhibition of DPP-4 and enhanced GLP-1 levels, is attenuated by co-ingestion with lectin-rich protein

et al. 2020

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Dipeptidyl peptidase-4 (DPP-4) is a proteolytic enzyme responsible for the rapid degradation of Glucagon-like peptide 1 (GLP-1) that is required for the secretion of insulin. DPP-4 also influences activation of node like receptor family, pyrin domain containing 3 (NLRP3) inflammasome under diabetic conditions. Although several polyphenols are reported for various bioactivities, they are consumed as part of the food matrix and not in isolation. Horsegram (Macrotyloma uniflorum) is a rich source of myricetin (Myr) (35 μg/g flour), reported for its anti-hyperglycemic effect. In this investigation, we aimed to study the effect of Myr, singly, and in the presence of co-nutrient horsegram protein (HP) on DPP-4 activity and its consequential impact on GLP-1, insulin, and NLRP3 inflammasome in high-fat diet and single low dose streptozotocin (STZ)-induced diabetic male Wistar rats. In diabetic control (DC), the activity of DPP-4 and its expression were higher compared to treated groups. The consequential decrease in the circulating GLP-1 levels in the DC group, but not treated groups, further indicated the effectiveness of our test molecules under diabetic conditions. Specifically, Myr decreased DPP-4 activity and its expression levels with enhanced circulating GLP-1 and insulin levels. Myr administration also resulted in a lessening of diabetes-induced NLRP3 inflammasome activation and enhanced antioxidant enzyme activities. HP also proved to be efficient in reducing elevated blood glucose levels and enhancing antioxidant enzyme activities. However, Myr, in the presence of HP as a co-nutrient, had diminished capacity to inhibit DPP-4 and, consequently, reduced potential in ameliorating diabetic conditions. Myr proved to be a potent inhibitor of DPP-4 in vitro and in vivo, resulting in enhanced circulating GLP-1 and insulin levels, thereby improving diabetic conditions. Though Myr and HP, individually ameliorate diabetic conditions, their dietary combination had reduced efficiency.

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Amelioration of streptozotocin-induced type 2 diabetes mellitus in Wistar rats by arachidonic acid

Cited by 64 publications

References 43 publications

Arachidonic Acid Metabolites in Cardiovascular and Metabolic Diseases

Arachidonic Acid Metabolites in Cardiovascular and Metabolic Diseases

Anti-diabetic, diabetic neuropathy protective action and mechanism of action involving oxidative pathway of chlorogenic acid isolated from Selinum vaginatum roots in rats

Anti-hyperglycemic activity of myricetin, through inhibition of DPP-4 and enhanced GLP-1 levels, is attenuated by co-ingestion with lectin-rich protein

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